The microbiome and resistome of hospital sewage during passage through the community sewer system

Buelow, Elena; Bayjanov,; Rj, Willems; Mj, Bonten; Schmitt, Heike; W, van Schaik

doi:10.1101/216242

Cited by 3 publications

(3 citation statements)

References 65 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibiotic residues in hospital wastewaters may reach levels that potentially could be selective for resistant strains [25][26][27]. Several studies have accordingly shown that hospital effluents can provide a rich variety of known ARGs and resistant enteric pathogens [28][29][30][31][32]. It is therefore plausible that a more explorative analysis could lead to the discovery of novel ARGs, either easily accessible to pathogens or already circulating among pathogens undetected.…”

Section: Introductionmentioning

confidence: 99%

Sewage effluent from an Indian hospital harbors novel carbapenemases and integron-borne antibiotic resistance genes

et al. 2019

View full text Add to dashboard Cite

Background: Hospital wastewaters contain fecal material from a large number of individuals, of which many are undergoing antibiotic therapy. It is, thus, plausible that hospital wastewaters could provide opportunities to find novel carbapenemases and other resistance genes not yet described in clinical strains. Our aim was therefore to investigate the microbiota and antibiotic resistome of hospital effluent collected from the city of Mumbai, India, with a special focus on identifying novel carbapenemases. Results: Shotgun metagenomics revealed a total of 112 different mobile antibiotic resistance gene types, conferring resistance against almost all classes of antibiotics. Beta-lactamase genes, including encoding clinically important carbapenemases, such as NDM, VIM, IMP, KPC, and OXA-48, were abundant. NDM (0.9% relative abundance to 16S rRNA genes) was the most common carbapenemase gene, followed by OXA-58 (0.84% relative abundance to 16S rRNA genes). Among the investigated mobile genetic elements, class 1 integrons (11% relative abundance to 16S rRNA genes) were the most abundant. The genus Acinetobacter accounted for as many as 30% of the total 16S rRNA reads, with A. baumannii accounting for an estimated 2.5%. High throughput sequencing of amplified integron gene cassettes identified a novel functional variant of an IMP-type (proposed IMP-81) carbapenemase gene (eight aa substitutions) along with recently described novel resistance genes like sul4 and bla RSA1. Using a computational hidden Markov model, we detected 27 unique metallo-beta-lactamase (MBL) genes in the shotgun data, of which nine were novel subclass B1 genes, one novel subclass B2, and 10 novel subclass B3 genes. Six of the seven novel MBL genes were functional when expressed in Escherichia coli. Conclusion: By exploring hospital wastewater from India, our understanding of the diversity of carbapenemases has been extended. The study also demonstrates that the microbiota of hospital wastewater can serve as a reservoir of novel resistance genes, including previously uncharacterized carbapenemases with the potential to spread further.

show abstract

Section: Introductionmentioning

confidence: 99%

Sewage effluent from an Indian hospital harbors novel carbapenemases and integron-borne antibiotic resistance genes

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Wastewater is any water from different processing and manufacturing operations such as pharmaceuticals, agriculture, domestic sources, and healthcare centres, whose quality have been tainted by a high level of anthropogenic influence [7]. The hospital wastewater is however quite different from wastewater from other sources because it contains a vast number of micro-and macro-pollutants, which have been discharged from the different departments of hospitals including laboratories, theaters, laundries, research units and other notable sections [8].…”

Section: Wastewater From Healthcare Systems (Composition and Microbio...mentioning

confidence: 99%

“…In some instances, some pharmaceuticals present in hospital wastewater have been implicated in causing the disruption of the endocrine system, impairment of the reproductive system and sex reversal in some aquatic species [10]. The discharge of this wastewater has led to the build-up of nutrients in the receiving aquatic ecosystem, leading to eutrophication [7].…”

Section: Wastewater From Healthcare Systems (Composition and Microbio...mentioning

confidence: 99%

The Threat of Methicillin Resistant Staphylococcus aureus (MRSA) in the Aquatic Environment via Wastewater Generated from Healthcare Facilities

Olumide Adekanmbi,

Olamilekan Adesola,

Olutoyin Adeyemi

et al. 2023

Bacterial Infectious Diseases Annual Volume 2023

View full text Add to dashboard Cite

In most developing countries of the world and few advanced ones, wastewater are discharged into the environment without any form of treatment, thus exposing the general public to hazardous chemicals, residual antibiotics, heavy metals and so many antimicrobial compounds. This chapter deals with the threat posed by methicillin resistant Staphylococcus aureus (MRSA) introduced into the aquatic ecosystem via wastewater generated from the operations of healthcare facilities. It focuses more on the microbiology and composition of wastewater from the hospital environment, and the role they played as a stimulant for the development of resistance in bacteria, while also emphasizing their roles as important reservoirs of MRSA in the aquatic environment. The epidemiology of MRSA in wastewater discharge from low-middle and high -income countries was examined, with another dig at the public health significance of these organisms in the water environment. The concluding part dwells heavily on the management and control strategies from the authors’ perspective, and this includes the one-health approach and the enactment of Government policies to control the indiscriminate discharge of untreated wastewater from the healthcare settings into receiving water bodies.

show abstract

Monitoring of Drug-Resistance Rates Among Staphylococcusaureus and Enterococcusfaecalis Isolates from Hospital Wastewaters in a Northern Province of Iran

Beyraghdar,

Fozouni,

Pordeli

2024

Compr Health Biomed Stud

View full text Add to dashboard Cite

Background: Hospital wastewater is one of the most dangerous and important sources of the spread of opportunistic bacteria. It is also one of the main causes of multidrug-resistant (MDR) hospital infections. Objectives: Considering the importance of investigating such environmental contaminants, this study was conducted to determine the frequency and phenotypes of antibiotic resistance among two important Gram-positive pathogens isolated from hospital wastewater. Methods: Enterococcus faecalis and Staphylococcus aureus isolates were identified from 42 samples obtained from 14 hospital wastewaters using filtration methods, microbiological tests, and polymerase chain reaction (PCR) with a specific ddlE primer and the VITEK-2 card system. The antibiotic resistance patterns were investigated using the Kirby-Bauer test according to CLSI-2021 guidelines regarding antibiotic classes. Results: Enterococcus faecalis was identified as the most frequent (n = 65; 30.37%) Gram-positive isolate. The highest resistance and susceptibility rates among these isolates were related to cotrimoxazole (SXT) (84.62%) and linezolid (LZ) (90.77%), respectively. In addition, the highest resistance and susceptibility rates among S. aureus isolates were related to penicillin (P) (78.95%) and LZ (87.72%), respectively. The frequency of extensively drug-resistant (XDR) E. faecalis isolates was two times higher than that of S. aureus isolates (P = 0.01). Conclusions: The results of this study confirm the high prevalence of antibiotic-resistant E. faecalis and S. aureus in hospital wastewaters. Therefore, eliminating these environmental pollutants requires continuous and simultaneous monitoring of environmental and clinical samples while considering the issue of antibiotic resistance. Moreover, appropriate control measures should be taken to prevent the spread of linezolid-resistant isolates in antibiotic therapy.

show abstract

The microbiome and resistome of hospital sewage during passage through the community sewer system

Abstract: 2 1

Cited by 3 publications

References 65 publications

Sewage effluent from an Indian hospital harbors novel carbapenemases and integron-borne antibiotic resistance genes

Sewage effluent from an Indian hospital harbors novel carbapenemases and integron-borne antibiotic resistance genes

The Threat of Methicillin Resistant Staphylococcus aureus (MRSA) in the Aquatic Environment via Wastewater Generated from Healthcare Facilities

Monitoring of Drug-Resistance Rates Among <i>Staphylococcus</i><i>aureus</i> and <i>Enterococcus</i><i>faecalis</i> Isolates from Hospital Wastewaters in a Northern Province of Iran

Contact Info

Product

Resources

About