The microbiome in urological diseases

Lee, Kwang Woo; Song, Ho‐Yeon; Kim, Duk Yoon

doi:10.4111/icu.2020.61.4.338

Cited by 28 publications

(37 citation statements)

References 84 publications

(116 reference statements)

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“…However, as studies of next-generation sequencing (NGS) have been actively conducted recently, bacteria have been identified even in the urine of healthy people, and the urinary system is attracting attention as a reservoir of a new microbiome [ 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. The microbiome has been associated with a spectrum of urological diseases, from acute infection to cancer [ 16 ]. Among them, cystitis is thought to be the first condition to receive help through microbiome analysis such as NGS.…”

Section: Introductionmentioning

confidence: 99%

Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis

Yoo

Shin

Song

et al. 2021

JCM

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Traditionally, the diagnostic mainstay of recurrent urinary tract infection has been urinary culture. However, the causative uropathogen of recurrent cystitis has not been well established. Urine DNA next-generation sequencing (NGS) can provide additional information on these infections. Herein, we compared urine NGS results and urine cultures in patients with acute uncomplicated cystitis (AUC) and recurrent cystitis (RC), and evaluated the difference in microbiome patterns in the NGS results. Patients who underwent urine culture and NGS due to AUC or RC were retrospectively reviewed. All urine samples were collected via a transurethral catheter and studied utilizing a type of NGS called 16S ribosomal RNA gene amplification and sequencing. The sensitivity of urine NGS was significantly higher than that of conventional urine culture (69.0% vs. 16.7%, p < 0.05). The detection rate of urine NGS was slightly lower in the RC group than in the AUC group (67.7% vs. 72.7%). Microbiome diversity was significantly higher in the RC group compared to the AUC group (p = 0.007), and the microbiome composition was significantly different between the AUC and RC groups. In the urine NGS results, Pseudomonas, Acinetobacter, and Enterobacteriaceae were found in the AUC group, and Sphingomonas, Staphylococcus, Streptococcus, and Rothia spp. were detected in the RC group. Urine NGS can significantly increase the diagnostic sensitivity compared to traditional urine culture methods, especially in RC patients. AUC and RC patients had significant differences in bacterial diversity and patterns. Therefore, recurrent cystitis might be approached from a different perspective.

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Section: Introductionmentioning

confidence: 99%

Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis

Yoo

Shin

Song

et al. 2021

JCM

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“…Previous reports have shown gut dysbiosis in patients with IC/BPS [19, 20], but whether this dysbiosis modulates pelvic pain remains speculative. To address this question AOAH-deficient mice were gavaged with anaerobic cultures of human stool from healthy controls or IC/BPS patients.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Gut dysbiosis has been increasingly recognized as a key player in visceral pain, and recent studies have indicated altered fecal microbiota in women with IC/BPS as well as in male patients suffering an analogic condition, chronic prostatitis/chronic pelvic pain syndrome [17–20]. Visceral convergence of sensory inputs that drives pelvic organ crosstalk and the gut-brain axis provide mechanisms by which gut dysbiosis may influence IC/BPS urologic and cognitive symptoms [21–25].…”

Section: Introductionmentioning

confidence: 99%

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Acyloxyacyl Hydrolase is a Host Determinant of Gut Microbiome-Mediated Pelvic Pain

Rahman-Enyart

Yang

Yaggie

et al. 2021

Preprint

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Dysbiosis of gut microbiota is associated with many pathologies, yet host factors modulating microbiota remain unclear. Interstitial cystitis/bladder pain syndrome (IC/BPS or “IC”) is a debilitating condition of chronic pelvic pain often with co-morbid urinary dysfunction and anxiety/depression, and recent studies find fecal dysbiosis in IC/BPS patients. We previously identified the locus encoding acyloxyacyl hydrolase, Aoah, as a modulator of pelvic pain severity in a murine IC/BPS model. AOAH-deficient mice spontaneously develop rodent correlates of pelvic pain, increased responses to induced pelvic pain models, voiding dysfunction, and anxious/depressive behaviors. Here, we report that AOAH-deficient mice exhibit dysbiosis of GI microbiota. AOAH-deficient mice exhibit an enlarged cecum, a phenotype long associated with germ-free rodents, and reduced trans-epithelial electrical resistance consistent with a “leaky gut” phenotype. AOAH-deficient ceca showed altered gene expression consistent with inflammation, Wnt signaling, and urologic disease. 16S rRNA sequencing of stool revealed altered microbiota in AOAH-deficient mice, and GC-MS identified altered metabolomes. Co-housing AOAH-deficient mice with wild type mice resulted in converged microbiota and altered predicted metagenomes. Co-housing also abrogated the pelvic pain phenotype of AOAH-deficient mice, which was corroborated by oral gavage of AOAH-deficient mice with stool slurry of wild type mice. Converged microbiota also alleviated comorbid anxiety-like behavior in AOAH-deficient mice. Oral gavage of AOAH-deficient mice with anaerobes cultured from IC/BPS stool resulted in exacerbation of pelvic allodynia. Together, these data indicate that AOAH is a host determinant of normal gut microbiota, and the dysbiosis associated with AOAH deficiency contributes to pelvic pain. These findings suggest that the gut microbiome is a potential therapeutic target for IC/BPS.

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“…2 Moreover there is no complete consensus on the urine sample collection method as many variations have been suggested in different studies. [3][4][5][6][7][8][9] Recently, it was shown that 16S ribosomal RNA sequencing can detect bacteria residing in the urinary tract from the urinary samples with a high sensitivity and specifity, even if the patient has UTI symptoms or not. 2,10 Therefore, we have decided to investigate the reliability of a two-step urine collection method and the validity of the hypothesis which argues that the first voided urine reflects the urethra and the midstream urine reflects the bladder.…”

Section: Introductionmentioning

confidence: 99%

Urinary microbiota; Which non‐ınvasive urine collection method should we use?

Özer

Yıldız²,

Incir

et al. 2021

Int J Clin Pract

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Objective: The aim of this study is to establish the optimal non-invasive urine sample collection method for the microbiota studies.Methodology: Twelve men with bladder carcinoma underwent first voided and midstream urine collection. Urine samples were analysed using V3-V4 regions of bacterial 16s ribosomal RNAs. Bacterial groups with relative abundance above 1% were analysed in first voided urine and midstream urine samples at phylum, class, order and family level. At the genus level, all of the identified bacterial groups' relative abundances were analysed. The statistical significance (P < .05) of differences between first voided and midstream urine sample microbiota was evaluated using the Wilcoxon test.Results: According to the analysis, 8 phyla, 14 class, 23 orders, 39 families and 29 different genera were identified in the first voided and the midstream urine samples. Statistical differences were not identified between first voided and midstream urine samples of all bacteria groups except the Clostridiales at order level (p:0.04) and Clostridia at class level (P: .04). Conclusions:Either first voided or midstream urine samples can be used in urinary microbiota studies as we determined that there is no statistically significant difference between them regarding the results of 16s ribosomal RNA analysis.

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The microbiome in urological diseases

Cited by 28 publications

References 84 publications

Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis

Urinary Microbiome Characteristics in Female Patients with Acute Uncomplicated Cystitis and Recurrent Cystitis

Acyloxyacyl Hydrolase is a Host Determinant of Gut Microbiome-Mediated Pelvic Pain

Urinary microbiota; Which non‐ınvasive urine collection method should we use?

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