The microbiome of the ice-capped Cayambe Volcanic Complex in Ecuador

Ruiz, Leonor Pérez; Monfort-Lanzas, Pablo; Quiroz-Moreno, Cristian; Rivadeneira, Erika; Castillejo, Pablo; Arnau, Vicente; Díaz, Wladimiro; Agathos, Spiros N.; Sangari, Félix J.; Jarrín‐V, Pablo; Molina, C. Alfonso

doi:10.3389/fmicb.2023.1154815

Cited by 3 publications

(1 citation statement)

References 110 publications

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“…(2) A control library that used DNA-free water as the input for library formation and subsequent sequencing instead of using the extraction process (Díaz et al, 2023).…”

Section: Sample Collection Experimental Design and Processingmentioning

confidence: 99%

Bacterial community changes in strawberry fruits (Fragaria × ananassa variety “Monterey”) from farm field to retail market stands, an indicator of postharvest contamination

Tenea,

Reyes

2024

Front. Microbiol.

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BackgroundStrawberry (Fragaria × ananassa) fruits are vulnerable to bacterial contamination; some species are pathogenic and can affect human health. Comprehending the bacterial composition and diversity at different ripe stages is a key determinant of the fruit health, productivity, and quality.MethodologyAn amplicon metagenomic approach on the 16S rRNA region was used to identify the bacterial diversity in exocarp of fruits collected from a farm field at two ripe stages: breaking (white, phase two) and ripe (red, phase four) and purchased from different retail market stands at ripe (red, phase four, ready-to-eat) stage. Besides, the fruit quality was assessed.ResultsStrawberries carries a high microorganisms diversity, with Pseudomonaceae, Yearsiniaceae, and Hafniaceae being the most abundant families across the samples. Among the groups, Pseudomonaceae and Clostridiaceae were the most abundant families at breaking (phase two) and ripe (phase four), whereas Yearsiniaceae, Hafniaceae, Aeromonadaceae, and Streptococcaceae were the most abundant families in the market group. Although samples from group four-field and market were at the same ripe stage, the bacterial species composition was divergent. Serratia spp. were prevalent (above 60%) in samples collected from the market group, and Pseudomonas (above 70%) species were mostly found in the samples collected from the field settings regardless of the phase. Besides, Escherichia coli and Salmonella enterica were detected in the ready-to-eat samples from both the field and the market, while Enterococcus gallinarum was detected in the samples that originated from the market. Interestingly, Shewanella putrefaciens and Shewanella profunda, two human opportunistic pathogens, were detected in the fruits from the market only. According to alpha and beta diversity analyses, strawberry fruits displayed significant differences (P < 0.05) in bacterial communities within the ripe group, with the samples from the market showing the most bacterial diversity. Although we do not directly correlate the quality attributes with bacterial diversity, the results indicated a clear separation between groups according with their ripe stage and origin.ConclusionThis study provides a comprehensive framework of the bacterial diversity throughout the transition from unripe to ripe strawberries which may aid in the development of preventative measures to manage the postharvest contamination.

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“…(2) A control library that used DNA-free water as the input for library formation and subsequent sequencing instead of using the extraction process (Díaz et al, 2023).…”

Section: Sample Collection Experimental Design and Processingmentioning

confidence: 99%

Bacterial community changes in strawberry fruits (Fragaria × ananassa variety “Monterey”) from farm field to retail market stands, an indicator of postharvest contamination

Tenea,

Reyes

2024

Front. Microbiol.

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Bacterial community assessment of drinking water and downstream distribution systems in highland localities of Ecuador

Molina,

Quiroz-Moreno,

Jarrín-V.

et al. 2024

Journal of Water and Health

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Bacterial communities in drinking water provide a gauge to measure quality and confer insights into public health. In contrast to urban systems, water treatment in rural areas is not adequately monitored and could become a health risk. We performed 16S rRNA amplicon sequencing to analyze the microbiome present in the water treatment plants at two rural communities, one city, and the downstream water for human consumption in schools and reservoirs in the Andean highlands of Ecuador. We tested the effect of water treatment on the diversity and composition of bacterial communities. A set of physicochemical variables in the sampled water was evaluated and correlated with the structure of the observed bacterial communities. Predominant bacteria in the analyzed communities belonged to Proteobacteria and Actinobacteria. Sphingobium genus, a chlorine resistance group, was particularly abundant. Of health concern in drinking water reservoirs were Fusobacteriaceae, Lachnospiraceae, and Ruminococcaceae; these families are associated with human and poultry fecal contamination. We propose the latter families as relevant biomarkers for establishing local standards for the monitoring of potable water systems in highlands of Ecuador. Our assessment of bacterial community composition in water systems in the Ecuadorian highlands provides a technical background to inform management decisions.

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A Protocol for the Characterization of Diatom Communities in Mountain Glaciers

Chamorro,

Borrego-Ramos,

Silva

et al. 2024

Water

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This research presents a significant contribution to the methodologies and protocols for studying diatom communities in cryoconite holes on glaciers. Cryoconite holes are unique microenvironments found on glacial surfaces that support intricate microbial ecosystems, with diatoms playing a pivotal role in these communities. The refined methodologies developed in this study include optimizing sampling techniques to ensure that collections are both representative and diverse, which is crucial for accurate ecological assessments. Additionally, advanced digestion processes have been implemented to effectively isolate and purify diatom samples while minimizing contaminants, thereby improving sample integrity. Improved microscopic mounting techniques enhance visual clarity, facilitating more precise identifications of diatoms under the microscope. Furthermore, integrating DNA-based taxonomy broadens the taxonomic scope, providing valuable molecular insights into the diversity and evolutionary relationships of diatoms. Collectively, these protocols aim to enhance the reliability, depth, and multidimensional understanding of diatom ecology in cryoconite holes and broader glacial ecosystems, ultimately contributing to the field of glaciology and microbial ecology.

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The microbiome of the ice-capped Cayambe Volcanic Complex in Ecuador

Cited by 3 publications

References 110 publications

Bacterial community changes in strawberry fruits (Fragaria × ananassa variety “Monterey”) from farm field to retail market stands, an indicator of postharvest contamination

Bacterial community changes in strawberry fruits (Fragaria × ananassa variety “Monterey”) from farm field to retail market stands, an indicator of postharvest contamination

Bacterial community assessment of drinking water and downstream distribution systems in highland localities of Ecuador

A Protocol for the Characterization of Diatom Communities in Mountain Glaciers

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