The microbiota of farmed mink (Neovison vison) follows a successional development and is affected by early life antibiotic exposure

Bahl, Martin Iain; Honoré, Anabelle Legarth; Skønager, Sanne Tygesen; Honoré, Oliver Legarth; Clausen, T.; Andresen, Lars Ole; Hammer, Anne Sofie

doi:10.1038/s41598-020-77417-z

Cited by 8 publications

(3 citation statements)

References 40 publications

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“…On commercial mink farms, intestinal diseases such as enteritis and diarrhea are a considerable threat to the health, growth, and survival of minks during their developing period ( 2 ). Traditionally, antibiotics have been widely used to prevent these intestinal diseases ( 3 ), which promote the growth of the animals. However, the misuse of antibiotics has resulted in the emergence of antibiotic-resistant bacteria and genes, reducing their therapeutic efficacy against diseases in both humans and animals ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation of Enterococcus faecium postbiotics on growth performance and intestinal health of growing male mink

Cao,

Sun,

Ren

et al. 2024

Front. Vet. Sci.

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Recent studies have demonstrated that postbiotics possess bioactivities comparable to those of probiotics. Therefore, our experiment aimed to evaluate the effects of postbiotics derived from Enterococcus faecium on the growth performance and intestinal health of growing male minks. A total of 120 growing male minks were randomly assigned to 4 groups, each with 15 replicates of 2 minks. The minks in the 4 groups were fed a basal diet supplemented with 0 (control), 0.05, 0.1, and 0.15% postbiotics derived from E. faecium (PEF), respectively. Compared to the control, PEF improved feed/gain (F/G) during the first 4 weeks and the entire 8 weeks of the study (p < 0.05); in addition, 0.1% PEF improved average daily gain (ADG) during the first 4 weeks and the entire 8 weeks of the study (p < 0.05), while 0.15% PEF improved ADG during the first 4 weeks of the study (p < 0.05). Consequently, 0.1% PEF minks displayed greater body weight (BW) at weeks 4 and 8 (p < 0.05), and 0.15% PEF minks had greater BW at week 4 (p < 0.05) than minks in the control. Furthermore, compared to the control, both 0.05 and 0.1% PEF enhanced the apparent digestibility of crude protein (CP) and ether extract (EE) (p < 0.05) in the initial 4 weeks, while both 0.1 and 0.15% PEF enhanced the apparent digestibility of CP and DM in the final 4 weeks (p < 0.05). Additionally, trypsin activity was elevated in the 0.1 and 0.15% PEF groups compared to the control (p < 0.05). In terms of intestinal morphology, PEF increased the villus height and villus/crypt (V/C) in the jejunum (p < 0.05), and both 0.1 and 0.15% PEF decreased the crypt depth and increased the villus height and V/C in the duodenum (p < 0.05) compared to the control group. Supplementation with 0.1% PEF increased the SIgA levels but decreased the IL-2, IL-8, and TNF-α levels in the jejunum (p < 0.05). Compared to the control, E. faecium postbiotics decreased the relative abundances of Serratia and Fusobacterium (p < 0.05). In conclusion, the results indicate that the growth performance, digestibility, immunity, and intestine development of minks are considerably affected by E. faecium postbiotics. In particular, dietary supplementation with 0.1% E. faecium postbiotics provides greater benefits than supplementation with 0.05 and 0.15%.

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Section: Introductionmentioning

confidence: 99%

Effects of dietary supplementation of Enterococcus faecium postbiotics on growth performance and intestinal health of growing male mink

Cao,

Sun,

Ren

et al. 2024

Front. Vet. Sci.

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“…However, the gut microbiota of weasels and polecats has not been adequately studied. Most studies on the gut microbiota of the Mustelidae family have focused on minks and otters [2,3]. The mink (Neovison vison, hereafter referred to as mink) is an economically important animal, with its fur having the main commercial value.…”

Section: Introductionmentioning

confidence: 99%

Biological Characterization and Whole-Genome Analysis of Bacillus subtilis MG-1 Isolated from Mink Fecal Samples

Ren,

Yu,

et al. 2023

Microorganisms

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Bacillus subtilis is an important part of the gut microbiota and a commonly used probiotic. In the present study, to assess the biological characteristics and probiotic properties of B. subtilis derived from mink, we isolated B. subtilis MG-1 isolate from mink fecal samples, characterized its biological characteristics, optimized the hydrolysis of casein by its crude extract, and comprehensively analyzed its potential as a probiotic in combination with whole-genome sequencing. Biological characteristics indicate that, under low-pH conditions (pH 2), B. subtilis MG-1 can still maintain a survival rate of 64.75%; under the conditions of intestinal fluid, gastric acid, and a temperature of 70 °C, the survival rate was increased by 3, 1.15 and 1.17 times compared with the control group, respectively. This shows that it can tolerate severe environments. The results of hydrolyzed casein in vitro showed that the crude bacterial extract of isolate MG-1 exhibited casein hydrolyzing activity (21.56 U/mL); the enzyme activity increased to 32.04 U/mL under optimized reaction conditions. The complete genome sequencing of B. subtilis MG-1 was performed using the PacBio third-generation sequencing platform. Gene annotation analysis results revealed that B. subtilis MG-1 was enriched in several Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways, and most genes were related to Brite hierarchy pathways (1485–35.31%) and metabolism pathways (1395–33.17%). The egg-NOG annotation revealed that most genes were related to energy production and conversion (185–4.10%), amino acid transport and metabolism (288–6.38%), carbohydrate transport and metabolism (269–5.96%), transcription (294–6.52%), and cell wall/membrane/envelope biogenesis (231–5.12%). Gene Ontology (GO) annotation elucidated that most genes were related to biological processes (8230–45.62%), cellular processes (3582–19.86%), and molecular processes (6228–34.52%). Moreover, the genome of B. subtilis MG-1 was predicted to possess 77 transporter-related genes. This study demonstrates that B. subtilis MG-1 has potential for use as a probiotic, and further studies should be performed to develop it as a probiotic additive in animal feed to promote animal health.

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“…Перспективными признаны технологии, позволяющие применять непищевое сырье, в частности, разработаны препараты, представляющие собой смесь низкомолекулярных пептидов и аминокислот [10].…”

Section: Introductionunclassified

Comparative evaluation of methods for studying the degree of assimilation of feed by animals

Белякова

Уша

2021

Agrarnaâ nauka

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Relevance. The activity of the digestive organs is largely determined by the feeding regime, in particular, the frequency, timeliness, and the order of feeding the feed. The digestibility of nutrients is influenced by the preparation of feed for feeding, which facilitates mechanical processing in the digestive tract and the action of digestive enzymes. Against the background of a decrease in the natural resistance of the animal body, an important aspect is the use of mineral supplements and the enrichment of diets with biologically active substances that increase the nutritional value and the degree of assimilation of feed by animals. The aim of the work was a comparative assessment of methods for studying the degree of assimilation of feed by animals.Methods. To determine the degree of food assimilation, 2 drops of isotonic (0.9%) sodium chloride solution and a small amount of intestinal contents were applied to a glass slide, a thin smear was prepared, dried in air for 30 min, and large particles were removed. The preparations were fixed for 15 min with 96% ethyl alcohol, then 3–5 drops of a 1.0% aqueous solution of methylene blue, Lugol, Sudan III were applied. Along with the indicated conventional methods, we tested a method for preparing preparations using fixation in vapors of a 25.0% solution of glutaraldehyde for 30–40 minutes, in vapors of a 1.0% aqueous solution of osmium tetroxide.Results. The kinetics of the formation of intestinal microbiocenoses is characterized by a decrease in the number of lactobacilli and an increase in the number of pathogenic and opportunistic bacteria. In a comparative assessment of the preparation methods, the advantage of microscopic research methods is the possibility of a detailed study of the contents, the degree of assimilation of feed components in the gastrointestinal tract, and a visual assessment of the state of the intestinal microflora. The advantage of the method for preparing preparations with the use of vapor fixation of a 25.0% solution of glutaraldehyde and osmium tetroxide is the possibility of visualizing the formation of monospecific and polyspecies biofilms by optical and scanning electron microscopy. The preparations were sufficiently contrasting, there were no artifacts, bacteria and fungi stained brown, which made it possible to count even pinpoint colonies using a magnifying glass or an optical microscope. The easily digestible feed results in a low fecal yield and a stable consistency. The use of functional food “Pristine Gold Vet” in the nutrition of dogs and fur-bearing animals made it possible to increase the absorption of nutrients by 18,1% and 15,3%.

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The microbiota of farmed mink (Neovison vison) follows a successional development and is affected by early life antibiotic exposure

Cited by 8 publications

References 40 publications

Effects of dietary supplementation of Enterococcus faecium postbiotics on growth performance and intestinal health of growing male mink

Effects of dietary supplementation of Enterococcus faecium postbiotics on growth performance and intestinal health of growing male mink

Biological Characterization and Whole-Genome Analysis of Bacillus subtilis MG-1 Isolated from Mink Fecal Samples

Comparative evaluation of methods for studying the degree of assimilation of feed by animals

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