The microinjection of a cannabinoid agonist into the accumbens shell induces anxiogenesis in the elevated plus-maze

Kochenborger, Larissa; Levone, Brunno Rocha; Silva, Eduardo Simão da; Taschetto, Ana Paula Dambros; Terenzi, Mariana Graciela; Paschoalini, Marta Aparecida; Faria, Moacir Serralvo

doi:10.1016/j.pbb.2014.05.017

Cited by 9 publications

(6 citation statements)

References 77 publications

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“… 14 The elevated plus-maze (EPM), a well-established animal test causing a fear status by comprehensible stimuli and the use of innate behavior of animals, is one of the most widely used models to assess anxiety in small rodents, and is a validated and reliable test for detecting both anxiolytic- and anxiogenic-like effects of agents. 15 , 16 In this animal model, an anxiolytic effect is evaluated by the relation of the entries into the open arms to the total entries and the time spent on the open arms of the EPM, in comparison with the same parameters of the control group. An increase of the time and proportion of the entries into the open arms without a changed locomotor activity is regarded as a powerful marker for an anxiolytic substance effect.…”

Section: Introductionmentioning

confidence: 99%

Study of the effect of extract of Thymus vulgaris on anxiety in male rats

Komaki‬

Hoseini

Shahidi

et al. 2016

Journal of Traditional and Complementary Medicine

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There is some evidence in traditional medicine for the effectiveness of Thymus vulgaris (百里香 bǎi lǐ xiāng) in the treatment of anxiety in humans. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of extract of T. vulgaris on rat behavior in the EPM. In the present study, the data were obtained from male Wistar rats. Animals were divided into four groups: saline group and T. vulgaris groups (50 mg/kg, 100 mg/kg, and 200 mg/kg infusion for 7 days by feeding). During the test period, the total distance covered by animals, the number of open- and closed-arm entries, and the time spent in open and closed arms of the EPM were recorded. T. vulgaris increased open-arm exploration and open-arm entry in the EPM, whereas extract of this plant has no effects on the total distance covered by animals and the number of closed-arm entries. The results of the present experiment indicate that T. vulgaris may have an anxiolytic profile in rat behavior in the EPM test, which is not influenced by the locomotor activity. Further research is required to determine the mechanisms by which T. vulgaris extract exerts an anxiolytic effect in rats.

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Section: Introductionmentioning

confidence: 99%

Study of the effect of extract of Thymus vulgaris on anxiety in male rats

Komaki‬

Hoseini

Shahidi

et al. 2016

Journal of Traditional and Complementary Medicine

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“…The EPM is a widely used behavioral test for assessing antianxiety behavior in rodent [17]. It is an established and reliable test for detecting anxiolytic-like effect of agents [18,19]. a powerful marker for an anxiolytic substance effect [20].…”

Section: Discussionmentioning

confidence: 99%

Experimental Study on Swiss White Mice to Evaluate the Antianxiety Effect of Common Wild Fig Extract (Ficus Thonningii)

Wadioni¹

2019

Innovare J Med Sci

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Objective: Since prehistoric times, man has relied on herbal plants as a source of medicine in curing various ailments. This study was carried out to assess the antianxiety-like effect of the ethanol extract of wild fig (Ficus thonningii) in Swiss white (CD-1) mice. Methods: Thirty Swiss mice were randomly divided into three groups (1–3) (n=10). Group 1 (control) was administered normal rat fed and saline, while Group 2 (low dose) and Group 3 (high dose) were administered 10 mg/kg w/w and 20 mg/kg w/w of the plant extract, respectively. The elevated plus maze was used to assess anxiety-related behaviors. Results: Data were analyzed using the statistical tools (one-way ANOVA and student t-test). Results revealed anxiety-like behavior in the low- and high-dose-treated mice when compared to the control. Conclusion: Therefore, administration of F. thonningii reduced an anxiety-like behavior in mice.

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“…criteria (Kochenborger et al, 2014;Allah Yar et al, 2015;Amos-Kroohs et al, 2015;Banaskowski et al, 2015;Bentea et al, 2015;Bernard et al, 2015;Bertolus et al, 2015;Brown et al, 2015;Bruining et al, 2015;Casarrubea et al, 2015Casarrubea et al, , 2016Chao et al, 2015;Colla et al, 2015;Cox et al, 2015;Daher and Mattioli, 2015; Dutra-Tavares et al, 2015; Finlay et al, 2015; Fowler and Muma, 2015; Gamberini et al, 2015;Goes et al, 2015;Gray and Hughes, 2015;Haleem et al, 2015;Hill et al, 2015;Horii and Kawaguchi, 2015;Iqbal et al, 2015;Kalouda and Pitsikas, 2015;Kawasaki et al, 2015Kawasaki et al, , 2017Ketcha Wanda et al, 2015;Kigar et al, 2015;Kumar et al, 2015;Langley et al, 2015;Lawther et al, 2015;Lecorps and Féron, 2015;Lee J. et al, 2015;Lee K. M. et al, 2015;Listowska et al, 2015;Liu et al, 2015;Livingston-Thomas et al, 2015;Mascarenhas et al, 2015;Mcneilly et al, 2015;Noguerón-Merino et al, 2015;Nunes et al, 2015;Pereda et al, 2015;…”

Section: Acknowledgmentsmentioning

confidence: 99%

Under or Absent Reporting of Light Stimuli in Testing of Anxiety-Like Behaviors in Rodents: The Need for Standardization

Neuwirth

Verrengia

Harikinish-Murrary

et al. 2022

Front. Mol. Neurosci.

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Behavioral neuroscience tests such as the Light/Dark Test, the Open Field Test, the Elevated Plus Maze Test, and the Three Chamber Social Interaction Test have become both essential and widely used behavioral tests for transgenic and pre-clinical models for drug screening and testing. However, as fast as the field has evolved and the contemporaneous involvement of technology, little assessment of the literature has been done to ensure that these behavioral neuroscience tests that are crucial to pre-clinical testing have well-controlled ethological motivation by the use of lighting (i.e., Lux). In the present review paper, N = 420 manuscripts were examined from 2015 to 2019 as a sample set (i.e., n = ~20–22 publications per year) and it was found that only a meager n = 50 publications (i.e., 11.9% of the publications sampled) met the criteria for proper anxiogenic and anxiolytic Lux reported. These findings illustrate a serious concern that behavioral neuroscience papers are not being vetted properly at the journal review level and are being released into the literature and public domain making it difficult to assess the quality of the science being reported. This creates a real need for standardizing the use of Lux in all publications on behavioral neuroscience techniques within the field to ensure that contributions are meaningful, avoid unnecessary duplication, and ultimately would serve to create a more efficient process within the pre-clinical screening/testing for drugs that serve as anxiolytic compounds that would prove more useful than what prior decades of work have produced. It is suggested that improving the standardization of the use and reporting of Lux in behavioral neuroscience tests and the standardization of peer-review processes overseeing the proper documentation of these methodological approaches in manuscripts could serve to advance pre-clinical testing for effective anxiolytic drugs. This report serves to highlight this concern and proposes strategies to proactively remedy them as the field moves forward for decades to come.

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The microinjection of a cannabinoid agonist into the accumbens shell induces anxiogenesis in the elevated plus-maze

Cited by 9 publications

References 77 publications

Study of the effect of extract of Thymus vulgaris on anxiety in male rats

Study of the effect of extract of Thymus vulgaris on anxiety in male rats

Experimental Study on Swiss White Mice to Evaluate the Antianxiety Effect of Common Wild Fig Extract (Ficus Thonningii)

Under or Absent Reporting of Light Stimuli in Testing of Anxiety-Like Behaviors in Rodents: The Need for Standardization

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