The Micromanipulation of Farm Animal Embryos

Picard, L.

doi:10.1016/b978-0-08-034730-1.50012-0

Cited by 4 publications

(1 citation statement)

References 116 publications

(98 reference statements)

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“…Since the first reports of embryo bisection in cattle Ozil et al, 1982), the technique has been used in this species to produce identical twins (Willadsen and Polge, 1981;Willadsen andBredbacka, 1996), facilitate embryo sexing (Picard et al, 1985 andBredbacka et al, 1994), and increase the number of transferable embryos (Leibo and Pall, 1987;Gray et al, 1991 andKippax et al, 1991), as has been reviewed (Picard and Betteridge 1989;Willadsen, 1982).…”

Section: Introductionmentioning

confidence: 99%

Viability of Bisected Rabbit Embryos After Cryopreservation Using Vitrification

2012

Journal of Productivity and Development

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To study the effects of developmental capacity after in vitro culture of intact and demi-embryos of rabbit in fresh case or after cryopreservation using vitrification technique, total 89 embryos at morula and blastocyst stages recovered from 16 does. Compaction rabbit embryos maintained intact (control) were compared with bisected embryos using a simplified splitting protocol at fresh or vitrified thawed embryos. The survivability of intact embryos and demi-embryos were evaluated after 24 hours of in vitro culture by development ability and morphological assessment. The percentage of development after 24 h of in vitro culture was higher (P<0.05) in fresh intact embryos than in fresh bisected embryos, either at morula (80 vs. 56%) or blastocyst (84.6 vs. 63.6%) stages. In case of intact or bisected embryos, percentage of development after 24 h of in vitro culture was insignificantly higher in embryos at blastocyst than at morula stages. The percentage of development after 24 h of in vitro culture was higher (P<0.05) for vitrified intact embryos than for vitrified bisected embryos, either at morula (61.5 vs. 45.0%) or blastocyst (75.0 vs. 42.1%) stages. In case of intact embryos, percentage of development after 24 h of in vitro culture was higher (P<0.05) for embryos at blastocyst than at morula stages. However, the percentage of development after 24 h of in vitro culture was nearly similar in vitrified bisected embryos, either at morula or at blastocyst stages.In conclusion, bisection and vitrification can be used for rabbit embryos, but embryonic stage and cryopreservation are limiting factors in the success of these procedures.

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