The microRNA-205-5p is correlated to metastatic potential of 21T series: A breast cancer progression model

Abstract

MicroRNA is a class of noncoding RNAs able to base pair with complementary messenger RNA sequences, inhibiting their expression. These regulatory molecules play important roles in key cellular processes including cell proliferation, differentiation and response to DNA damage; changes in miRNA expression are a common feature of human cancers. To gain insights into the mechanisms involved in breast cancer progression we conducted a microRNA global expression analysis on a 21T series of cell lines obtained from the same patient during different stages of breast cancer progression. These stages are represented by cell lines derived from normal epithelial (H16N2), atypical ductal hyperplasia (21PT), primary in situ ductal carcinoma (21NT) and pleural effusion of a lung metastasis (21MT-1 and 21MT-2). In a global microRNA expression analysis, miR-205-5p was the only miRNA to display an important downregulation in the metastatic cell lines (21MT-1; 21MT-2) when compared to the non-invasive cells (21PT and 21NT). The lower amounts of miR-205-5p found also correlated with high histological grades biopsies and with higher invasion rates in a Boyden chamber assay. This work pinpoints miR-205-5p as a potential player in breast tumor invasiveness.

miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy

“…Many studies demonstrate that prostate cancer cell lines and tumor cells exhibit suppressed miR-205 levels [ 24 ]. miR-205 loss is highly associated with tumor growth, invasion, and migration of cancer cells [ 25 ]. The reconstitution of miR-205 effectively inhibits proliferation of prostate cancer cells ( Figure 3 D) [ 26 ].…”

3D hydrogel breast cancer models for studying the effects of hypoxia on epithelial to mesenchymal transition

“…In this study, we used isogenic primary 21PT and metastatic 21MT-2 cell lines, which were derived from the same HER2 positive patient at different tumor stages [ 51 – 53 ]. 21PT are noninvasive cell line and represent infiltrating ductal carcinoma with carcinoma in situ component, which represent early stage of breast cancer, whereas 21MT-2 is a metastatic cell line and is derived from pleural effusion of lung metastasis [ 54 , 55 ].…”

The Regulatory Role of MicroRNAs in Breast Cancer

“…miR-205-5p was also predicted to target genes responsible for breast cancer invasiveness, including SOCS3 , PTPRN2 , and MMP3 , and also genes associated with EMT regulatory functions, such as TGFB1 . Therefore, this work showed that miR-205-3p may serve as a key player during progression of a tumor to an invasive, metastatic phenotype [148].…”