The microRNA-29 plays a central role in osteosarcoma pathogenesis and progression

Zhang, W.; Qian, Jin; Yi, Hong; Yang, Zhangshuo; Wang, C. F.; Chen, J. Y.; Xing, Wei; Fu, Qiang; Ma, Hui

doi:10.1134/s0026893312040139

Cited by 37 publications

(38 citation statements)

References 26 publications

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“…Moreover, miRNA expression profiles in metastatic cancers are significantly different from non-metastatic cancers, and some miRNAs in metastatic tumors impact patient survival [Budhu et al, 2008]. Previous studies showed an association between OS pathogenesis and altered miRNA expression [Zhang et al, 2012;Miao et al, 2013]. A study found that miR-34 was decreased in OS tumor cells, and miR-34 target genes were involved in cell cycle arrest and apoptosis [He et al, 2009].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Xiao

Chen²,

Wang³

et al. 2015

Cytogenet Genome Res

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miR-150 expression in osteosarcoma (OS) cell lines and human osteoblast cells was detected, and OS cell models were transfected with exogenous miR-150 to investigate its role in cell proliferation, invasion, and apoptosis. Our results showed that miR-150 expression in OS cells (MG63, Saos-2, SOSP-9607, and U2OS) was significantly lower compared to the osteoblast hFOB1.19 cell line (all p < 0.01). The expression level of miR-150 in MG63 cells that were transfected with exogenous miR-150 mimics was markedly upregulated, while the miR-150 expression level in the inhibitor group was significantly downregulated (both p < 0.01). Similar results were also found in SOSP-9607 cells. Importantly, exogenous miR-150 expression stimulated cell apoptosis and inhibited proliferation, invasion, and migration. A luciferase reporter assay displayed that miR-150 also regulated Sp1 expression by targeting its 3′-UTR, and qRT-PCR and Western blotting showed that elevated levels of miR-150 may reduce Sp1 protein expression. The mRNA and protein levels of Sp1 were upregulated after transfection with a Sp1-expression plasmid and partially reversed the inhibitory effects of miR-150 on cell proliferation, invasion, and metastasis in MG63 and SOSP-9607 cells, as well as promoted cell apoptosis. In conclusion, miR-150 inhibits cell proliferation, invasion, and metastasis and stimulates cell apoptosis by regulating the expression of Sp1. Therefore, miR-150 may be a potential clinical target for the treatment of OS patients.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Xiao

Chen²,

Wang³

et al. 2015

Cytogenet Genome Res

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“…In the muscular and skeletal systems, miR-29 was found to be significantly downregulated in osteoblastoma (29) and rhabdomyosarcoma (30). MiR-29a induced osteoblastic cell apoptosis by silencing Bcl-2 and Mcl-1 and inducing E2F1 and E2F3 expression (31). In the genitourinary system, all three isoforms of miR-29 (a, b and c) are highly expressed in normal tissues.…”

Section: Expression Of Mirna-29 In Cancersmentioning

confidence: 98%

Diverse roles of miR-29 in cancer (Review)

et al. 2014

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microRNAs (miRNAs) are non-coding RNAs which have the capacity to regulate gene expression at the post-transcriptional level, and have emerging as key factors involved in cancer at all stages ranging from initiation to metastasis. In the present review, we summmarize the diverse roles of the microRNA-29 (miR-29) family in cancer. First, we present a concise introduction to the miR-29 family and the expression profile of miR-29 in various cancer types. We next highlight the upstream regulatory pathway of miR-29 and describe the relationship between miR-29 and cancer in detail. As a tumor suppressor, miR-29 restrains cancer progression by promoting tumor cell apoptosis, by suppressing DNA methylation of tumor-suppressor genes, by reducing proliferation of tumors and by increasing chemosensitivity. However, as a tumor promoter, miR-29 mediates epithelial-mesenchymal transition (EMT) and promotes metastasis in breast cancer and colon cancer. Finally, we suggest that miR-29 represents a novel diagnostic and prognostic biomarker or a therapeutic target for cancer. Our review highlights the diverse relationship between miR-29 and cancer (particularly digestive system neoplasms). Further research of miR-29 in cancer is warranted.

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“…Thus, miRNAs might be considered oncogenes or tumor suppressor genes in and of themselves. A wide variety of tumors have been found with altered miRNA expression, such as breast cancers [26], hepatocellular carcinomas [27], osteosarcomas [28] and many others [29]. Aberrant expression patterns in miRNAs have also been described in tumors that affect the nervous system, such as gliomas and meningiomas [30], [31].…”

Section: Introductionmentioning

confidence: 99%

Global Profiling in Vestibular Schwannomas Shows Critical Deregulation of MicroRNAs and Upregulation in Those Included in Chromosomal Region 14q32

Torres‐Martín

Lassaletta²,

Campos

et al. 2013

PLoS ONE

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BackgroundVestibular schwannomas are benign tumors that arise from Schwann cells in the VIII cranial pair and usually present NF2 gene mutations and/or loss of heterozygosity on chromosome 22q. Deregulation has also been found in several genes, such as ERBB2 and NRG1. MicroRNAs are non-coding RNAs approximately 21 to 23 nucleotides in length that regulate mRNAs, usually by degradation at the post-transcriptional level.MethodsWe used microarray technology to test the deregulation of miRNAs and other non-coding RNAs present in GeneChip miRNA 1.0 (Affymetrix) over 16 vestibular schwannomas and 3 control-nerves, validating 10 of them by qRT-PCR.FindingsOur results showed the deregulation of 174 miRNAs, including miR-10b, miR-206, miR-183 and miR-204, and the upregulation of miR-431, miR-221, miR-21 and miR-720, among others. The results also showed an aberrant expression of other non-coding RNAs. We also found a general upregulation of the miRNA cluster located at chromosome 14q32.ConclusionOur results suggest that several miRNAs are involved in tumor formation and/or maintenance and that global upregulation of the 14q32 chromosomal site contains miRNAs that may represent a therapeutic target for this neoplasm.

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The microRNA-29 plays a central role in osteosarcoma pathogenesis and progression

Cited by 37 publications

References 26 publications

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

MicroRNA-150 Inhibits Cell Invasion and Migration and Is Downregulated in Human Osteosarcoma

Diverse roles of miR-29 in cancer (Review)

Global Profiling in Vestibular Schwannomas Shows Critical Deregulation of MicroRNAs and Upregulation in Those Included in Chromosomal Region 14q32

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