The microRNA and the perspectives of miR-302

Chen, Emily Yen Yu; Chen, Jack S.; Ying, Shao‐Yao

doi:10.1016/j.heliyon.2019.e01167

Cited by 11 publications

(7 citation statements)

References 128 publications

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“…Consequently, miR-302a might be a biologically and analytically attractive biomarker for ASD with a severe symptomatic biomarker. Moreover, consistent findings have shown that the miR-302 family is critical in stem cell pluripotency and renewal, and somatic cell DNA demethylation [ 20 , 21 , 22 ]. miR-135b-5p was another miRNA that was flagged in our study as being expressed at significantly higher levels in individuals who expressed severe symptoms versus mild ones.…”

Section: Discussionmentioning

confidence: 90%

“…Interestingly we observed that the miR-302 family (hsa-miR-302a-5p, hsa-miR-302c-3p, hsa-miR-302a-3p, hsa-miR-302d-3p, hsa-miR-302b-3p, hsa-miR-302c-5p and hsa-miR-302b-5p) were expressed at significantly high levels in individuals that expressed severe ASD symptoms in comparison to those that expressed mild symptoms. Previous findings have shown that the miR-302 family is crucial in stem cell pluripotency and renewal and somatic cell DNA demethylation [ 20 , 21 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

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Circulating Non-Coding RNAs as a Signature of Autism Spectrum Disorder Symptomatology

Salloum-Asfar

Elsayed

Elhag

et al. 2021

IJMS

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Autism spectrum disorder (ASD) is a multifaced neurodevelopmental disorder that becomes apparent during early childhood development. The complexity of ASD makes clinically diagnosing the condition difficult. Consequently, by identifying the biomarkers associated with ASD severity and combining them with clinical diagnosis, one may better factionalize within the spectrum and devise more targeted therapeutic strategies. Currently, there are no reliable biomarkers that can be used for precise ASD diagnosis. Consequently, our pilot experimental cohort was subdivided into three groups: healthy controls, individuals those that express severe symptoms of ASD, and individuals that exhibit mild symptoms of ASD. Using next-generation sequencing, we were able to identify several circulating non-coding RNAs (cir-ncRNAs) in plasma. To the best of our knowledge, this study is the first to show that miRNAs, piRNAs, snoRNAs, Y-RNAs, tRNAs, and lncRNAs are stably expressed in plasma. Our data identify cir-ncRNAs that are specific to ASD. Furthermore, several of the identified cir-ncRNAs were explicitly associated with either the severe or mild groups. Hence, our findings suggest that cir-ncRNAs have the potential to be utilized as objective diagnostic biomarkers and clinical targets.

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Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Circulating Non-Coding RNAs as a Signature of Autism Spectrum Disorder Symptomatology

Salloum-Asfar

Elsayed

Elhag

et al. 2021

IJMS

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“…miR‐302e is a member of the miR‐302 cluster family, which also contains miR‐302a, miR‐302b, miR‐302c, and miR‐302d (Guo et al, ). Except for miR‐302e, other members in this family have been extensively studied and considered as tumor suppressors in many human cancers (Chen, Chen, & Ying, ; Li, Huo, Pan, Wang, & Ma, ). In our data, miR‐302e was observed to be significantly down‐regulated in NSCLC and identified as a tumor growth inhibitor.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA circ‐CMPK1 contributes to cell proliferation of non‐small cell lung cancer by elevating cyclin D1 via sponging miR‐302e

Dong

Qian

2019

Molec Gen & Gen Med

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Background: It is well recognized that competing endogenous RNA (ceRNA) regulatory network is linked to the development and progression of cancer, including non-small cell lung cancer (NSCLC). Herein, we aimed to explore the functional role of circ-CMPK1/miR-302e/cyclin D1 ceRNA signaling in NSCLC. Methods: GEO database (GSE10 2287) was utilized to screen differentially expressed miRNAs in NSCLC. Quantitative reverse transcription PCR (qRT-PCR) and western blotting assays were used to determine gene expression. Cell proliferation analysis was performed with Cell Counting Kit-8 (CCK-8) and cell cycle assays.Luciferase reporter and RNA pull-down assays were conducted to identify the interaction among circ-CMPK1, miR-302e, and cyclin D1. Xenograft tumor model was established to evaluate the role of circ-CMPK1/miR-302e/cyclin D1 axis in vivo. Results: miR-302e expression was significantly down-regulated in NSCLC cell lines and tissues and its decrease was closely associated with aggressive clinicopathological features and unfavorable outcome. Overexpression and knockdown of miR-302e obviously retarded and enhanced the growth of NSCLC, respectively. Furthermore, we found that miR-302 was sponged by circular RNA CMPK1 (circ-CMPK1, hsa_ circ_0012384), which was remarkably up-regulated in NSCLC and predicted poor prognosis. Circ-CMPK1 was capable to promote NSCLC cells proliferation by increasing the expression of cyclin D1 via inhibiting miR-302 activity. Moreover the miR-302e-mediated tumor inhibition could be effectively counteracted by ectopic expression of circ-CMPK1 or cyclin D1 both in vitro and in vivo. Conclusion: Our data demonstrate for the first time that circ-CMPK1/miR-302e/cyclin D1 signaling plays an essential regulatory role in NSCLC and targeting this axis may be an efficacious avenue for treatment of NSCLC patients. K E Y W O R D Sbiomarker, ceRNA, circular RNA, microRNA, NSCLC, proliferation 2 of 10 | CUI et al.

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“…miR‐302 family members are intronic and embryonic stem (ES) cell‐specific miRNAs. They play important roles in many biological processes; these processes include the pluripotency, self‐renewal, and reprogramming of ES cells, as well as antitumor activities (Chen et al, 2019). A study investigated the roles of the miR‐302 cluster in HT‐29 colorectal cancer cells, and it found that the miR‐302 cluster can modulate oncogenic properties through inhibition of proliferation, angiogenesis, and invasion, as well as reversal of the epithelial‐to‐mesenchymal transition (Maadi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

SOX‐17 is involved in invasion and apoptosis of colorectal cancer cells through regulating miR‐302b‐3p expression

et al. 2021

Cell Biology International

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The prognosis of advanced colorectal cancer (CRC) is currently still very poor, which suggests that the biological mechanisms of CRC oncogenesis are not fully understood. This study was conducted to explore the regulatory effect of SOX‐17 on the expression of microRNA (miR)‐302b‐3p, and the involvement of SOX‐17 in the invasion and apoptosis of CRC cells. The expression of SOX‐17 and miR‐302a,b,c,d‐3p in colorectal cancer and normal colon epithelial cell lines was measured by real‐time polymerase chain reaction and/or western blot. The regulatory effects of SOX‐17 on miR‐302b‐3p gene in HT29 and LoVo cells were tested using the ChiP assay. The biological activities of SOX‐17 and miR‐302b‐3p were evaluated by invasion and apoptosis assay. Results showed that transfection of SOX‐17 small interfering RNA (siSOX‐17) significantly increased, whereas transfection of SOX‐17 overexpression vector (oeSOX‐17) significantly decreased, miR‐302b expression in HT29 and LoVo cells. Cotransfection of oeSOX‐17 and miR‐302b‐3p inhibitor (INmiR‐302b) significantly blocked the effects of SOX‐17 in HT29 and LoVo cells. ChIP experiments showed that SOX‐17 bonded to the miR‐302b‐3p promoter in HT29 and LoVo cells. Transfection of oeSOX‐17 and miR‐302b‐3p mimics (MImiR‐302b) significantly decreased, whereas transfection of siSOX‐17 and INmiR‐302b significantly increased, the invasion of HT29 and LoVo cells. In contrast, transfection of oeSOX‐17 and MImiR‐302b significantly increased, while transfection of siSOX‐17 and INmiR‐302b significantly decreased, apoptosis in HT29 and LoVo cells. Cotransfection of oeSOX‐17 and INmiR‐302b significantly blocked the effects of oeSOX‐17 on cell invasion and apoptosis in HT29 and LoVo cells. These results suggested that SOX‐17 can bind to the promoter of miR‐302b‐3p gene to regulate its expression, while both SOX‐17 and miR‐302b regulate the invasion and apoptosis in colorectal cancer cells.

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The microRNA and the perspectives of miR-302

Cited by 11 publications

References 128 publications

Circulating Non-Coding RNAs as a Signature of Autism Spectrum Disorder Symptomatology

Circulating Non-Coding RNAs as a Signature of Autism Spectrum Disorder Symptomatology

Circular RNA circ‐CMPK1 contributes to cell proliferation of non‐small cell lung cancer by elevating cyclin D1 via sponging miR‐302e

SOX‐17 is involved in invasion and apoptosis of colorectal cancer cells through regulating miR‐302b‐3p expression

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