The microRNA expression signature on modified titanium implant surfaces influences genetic mechanisms leading to osteogenic differentiation

Chakravorty, Nishant; Ivanovski, Sašo; Prasadam, Indira; Crawford, Ross; Oloyede, Adekunle; Xiao, Yin

doi:10.1016/j.actbio.2012.05.008

Cited by 56 publications

(53 citation statements)

References 41 publications

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“…Nine studies found that specific surfaces induced osteoblast differentiation, expression of osteogenic genes or repression of the miRNAs that downregulate osteogenic genes [46][47][48]50,52,53,[58][59][60], while six articles did not indicate a better surface [49,51,[54][55][56][57]. Two articles documented similar trends in gene expression regardless of implant surface and found that the effect of time influenced gene expression more than the surfaces [49,54].…”

Section: Comparative Analysismentioning

confidence: 89%

“…Publication types of the included articles are expressed in Figure 2. Fifteen of 17 articles were in vitro or in vivo studies [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60]. One was a randomized clinical trial with immune-histochemical analysis [61], and the other was a narrative review [62].…”

Section: Resultsmentioning

confidence: 99%

“…Eight articles documented miRNA production [47,[51][52][53][55][56][57][58]. Seven articles did not provide information on miRNA production [46,[48][49][50]54,59,60].…”

Section: In Vivo and In Vitro Studiesmentioning

confidence: 99%

“…Regarding in vitro studies, four studies used human osteoblast like cells (MG-63) for cell culture [48,53,56,57] while the other four used human alveolar stem cells from human donors [51,52,55,59]. Human mesenchymal stem cells [60], dental pulp stem cells [47], rat bone marrow cells [58], and marrow stromal cells obtained from the iliac crest [46] were used in one study each.…”

Section: Cell Populationmentioning

confidence: 99%

“…Electrolytic etched surfaces were associated with higher levels of alkaline phosphatase, osteocalcin, Runx-2, osteopontin, and collagen type Iα1 [48]. Sandblasted and acid-etched (SLA) and modSLA surfaces downregulated miRNAs influencing osteoprogenitor cells [51]. CS/HA/miR-21 surfaces increased the production levels of collagen type IIIα1, osteocalcin, Runx-2, osteopontin, and collagen type Iα1 [52].…”

Section: Implant Surfacesmentioning

confidence: 99%

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The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

Gianfilippo¹,

Gianfilippo²,

Prato

2017

Epigenomes

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Cell phenotype is influenced by the linear sequence of bases and by epigenetic changes. Despite the huge number of implants placed every year, epigenetic mechanisms controlling peri-implant processes remain unexplored. The purpose of this systematic qualitative review was to investigate the available articles dealing with the relationships between DNA methylations, histone modifications, or micro-RNA (miRNA) production and implant therapy. A large variety of different surfaces were evaluated based on their osteogenic stimulation of osteoblasts. Dental implant treatments like potassium hydroxide (KOH) alkali-etching, electrolytic etching, ionization, functionalization with miRNAs or anti-miRNAs, or osteogenic peptides enhanced osteoblast differentiation and gene activation by regulating miRNA production. Zirconia and anatase coating inhibited the activation of osteogenic genes. Epigenetic changes on peri-implant cells induced by smoking still remain unclear. Due to the heterogeneity of methodologies, a meta-analysis was not possible. Even if it is impossible to define which implant surface was best to genetically stimulate osteogenesis, there is evidence that implant surface features can upregulate or downregulate genes related to osseointegration.

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Section: Comparative Analysismentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

“…Eight articles documented miRNA production [47,[51][52][53][55][56][57][58]. Seven articles did not provide information on miRNA production [46,[48][49][50]54,59,60].…”

Section: In Vivo and In Vitro Studiesmentioning

confidence: 99%

Section: Cell Populationmentioning

confidence: 99%

Section: Implant Surfacesmentioning

confidence: 99%

See 3 more Smart Citations

The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

Gianfilippo¹,

Gianfilippo²,

Prato

2017

Epigenomes

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Titanium surface hydrophilicity modulates the human macrophage inflammatory cytokine response

Alfarsi

Hamlet

Ivanovski

2013

J. Biomed. Mater. Res.

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124

108

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Increased titanium surface hydrophilicity has been shown to accelerate dental implant osseointegration. Macrophages are important in the early inflammatory response to surgical implant placement and influence the subsequent healing response. This study investigated the modulatory effect of a hydrophilic titanium surface on the inflammatory cytokine expression profile in a human macrophage cell line (THP-1). Genes for 84 cytokines, chemokines, and their receptors were analyzed following exposure to (1) polished (SMO), (2) micro-rough sand blasted, acid etched (SLA), and (3) hydrophilic-modified SLA (modSLA) titanium surfaces for 1 and 3 days. By day 3, the SLA surface elicited a pro-inflammatory response compared to the SMO surface with statistically significant up-regulation of 16 genes [Tumor necrosis factor (TNF) Interleukin (IL)-1β, Chemokine (C-C motif) ligand (CCL)-1, 2, 3, 4, 18, 19, and 20, Chemokine (C-X-C motif) ligand (CXCL)-1, 5, 8 and 12, Chemokine (C-C motif) receptor (CCR)-7, Lymphotoxin-beta (LTB), and Leukotriene B4 receptor (LTB4R)]. This effect was countered by the modSLA surface, which down-regulated the expression of 10 genes (TNF, IL-1α and β, CCL-1, 3, 19 and 20, CXCL-1 and 8, and IL-1 receptor type 1), while two were up-regulated (osteopontin and CCR5) compared to the SLA surface. These cytokine gene expression changes were confirmed by decreased levels of corresponding protein secretion in response to modSLA compared to SLA. These results show that a hydrophilic titanium surface can modulate human macrophage pro-inflammatory cytokine gene expression and protein secretion. An attenuated pro-inflammatory response may be an important molecular mechanism for faster and/or improved wound healing.

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Influence of osteocytes in the in vitro and in vivo β‐tricalcium phosphate‐stimulated osteogenesis

Chen

Yuen

et al. 2013

J Biomedical Materials Res

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Osteocytes, known to act as the main regulators of bone homeostasis, have become a major focus in the field of bone research. Bioactive ceramics have been widely used for bone regeneration. However, there are few studies about the interaction of osteocytes with bioceramics. The effects of osteocytes on the in vitro and in vivo osteogenesis of bioceramics are also unclear. The aim of this study was to investigate the role of osteocytes on the β-tricalcium phosphate (β-TCP) stimulated osteogenesis. It was found that osteocytes responded to the β-TCP stimulation, leading to the release of Wnt (wingless-related MMTV integration site), which enhanced osteogenic differentiation of bone marrow stromal cells via Wnt signaling pathway. Receptor activator of nuclear factor kappa B ligand, an osteoclast inducer, was also upregulated, indicating that osteocytes would also participated in activation of osteoclasts, which played a major role in the degradation process of β-TCP and new bone remodeling. In vivo studies further demonstrated that when the material was completely embedded by newly formed bone, the only cell contacting with the material was osteocyte. However, the material would eventually be degraded and replaced by the new bone, requiring the participation of osteoclasts and osteoblasts, which were demonstrated by using immunostaining in this study. As the only cell contacting with the material, osteocytes probably acted in a regulatory role to regulate the surrounding osteoclasts and osteoblasts. Osteocytes were also found to participate in the maturation of osteoblasts and the mineralization process of biomaterials, by upregulating E11 (podoplanin) and dentin matrix protein 1 expression. These findings indicated that osteocytes involved in bone biomaterial-mediated osteogenesis and biomaterial degradation, providing valuable insights into the mechanism of material-stimulated osteogenesis, and a novel strategy to optimize the evaluating system for the biological properties of biomaterials.

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The microRNA expression signature on modified titanium implant surfaces influences genetic mechanisms leading to osteogenic differentiation

Cited by 56 publications

References 41 publications

The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

The Role of Epigenetics on Dental Implant Therapy: A Systematic Review

Titanium surface hydrophilicity modulates the human macrophage inflammatory cytokine response

Influence of osteocytes in the in vitro and in vivo β‐tricalcium phosphate‐stimulated osteogenesis

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