The microRNA-producing enzyme Dicer1 is essential for zebrafish development

Wienholds, Erno; Koudijs, Marco J.; Eeden, Freek van; Cuppen, Edwin; Plasterk, Ronald H.A.

doi:10.1038/ng1251

Cited by 435 publications

(321 citation statements)

References 10 publications

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“…Specific miRNAs control specific tissue development at a specific developmental stage (Lagos-Quintana et al, 2002). Dicer mutant in zebrafish embryos developed normal at the beginning, but embryo development arrested 8 days after fertilization (Wienholds et al, 2003). This suggests that embryo development need appropriate expression of certain miRNAs.…”

Section: Functions Of Micrornas In Animalsmentioning

confidence: 99%

MicroRNAs and their regulatory roles in animals and plants

Zhang

Wang

Pan

2006

Journal Cellular Physiology

493

307

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microRNAs (miRNAs) are an abundant class of newly identified endogenous non-protein-coding small RNAs. They exist in animals, plants, and viruses, and play an important role in gene silencing. Translational repression, mRNA cleavage, and mRNA decay initiated by miRNA-directed deadenylation of targeted mRNAs are three mechanisms of miRNA-guided gene regulation at the posttranscriptional levels. Many miRNAs are highly conserved in animals and plants, suggesting that they play an essential function in plants and animals. Lots of investigations indicate that miRNAs are involved in multiple biological processes, including stem cell differentiation, organ development, phase change, signaling, disease, cancer, and response to biotic and abiotic environmental stresses. This review provides a general background and current advance on the discovery, history, biogenesis, genomics, mechanisms, and functions of miRNAs.

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Section: Functions Of Micrornas In Animalsmentioning

confidence: 99%

MicroRNAs and their regulatory roles in animals and plants

Zhang

Wang

Pan

2006

Journal Cellular Physiology

493

307

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“…14,15 Of note, Dicer-deficient mouse and zebrafish embryos are stalled at an early developmental stage, leading to developmental arrest, small size, morphological abnormalities and lethality. 16,17 Since either overexpression or underexpression of a variety of genes can promote cancer, it is not inconceivable that miRNAs may also have significant cancer-related effects. A constantly growing body of evidence indicates that miRNAs indeed play significant roles in cellular transformation and carcinogenesis, acting either as oncogenes or as tumor suppressors (reviewed in refs.…”

Section: Micrornasmentioning

confidence: 99%

Tiny Actors, Great Roles: microRNAs in p53's Service

Raver-Shapira¹,

Oren²

2007

Cell Cycle

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“…In addition, mouse embryos hypomorphic for Dicer die mid-gestation (14). Dicer-deficient zebrafish progress much further in development, probably owing to the presence of maternally deposited Dicer transcripts (15). Depletion of maternal Dicer achieved by germ-line transplantation in zebrafish revealed that Dicer is required for morphogenesis but not cell fate specification during zebrafish embryogenesis and that the absence of miRNAs is responsible, at least in part, for this phenotype (16).…”

mentioning

confidence: 99%

Characterization of Dicer-deficient murine embryonic stem cells

Murchison

Partridge

Tam

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

734

652

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Dicer is an RNase III-family nuclease that initiates RNA interference (RNAi) and related phenomena by generation of the small RNAs that determine the specificity of these gene silencing pathways. We have previously shown that Dicer is essential for mammalian development, with Dicer-deficient mice dying at embryonic day 7.5 with a lack of detectable multipotent stem cells. To permit a more detailed investigation of the biological roles of Dicer, we have generated embryonic stem cell lines in which their single Dicer gene can be conditionally inactivated. As expected, Dicer loss compromises maturation of microRNAs and leads to a defect in gene silencing triggered by long dsRNAs. However, the absence of Dicer does not affect the ability of small interfering RNAs to repress gene expression. Of interest, Dicer loss does compromise the proliferation of ES cells, possibly rationalizing the phenotype previously observed in Dicer-null animals. Dicer loss also affects the abundance of transcripts from mammalian centromeres but does so without a pronounced affect on histone modification status at pericentric repeats or methylation of centromeric DNA. These studies provide a conditional model of RNAi deficiency in mammals that will permit the dissection of the biological roles of the RNAi machinery in cultured mammalian cells.centromeres ͉ RNA interference

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The microRNA-producing enzyme Dicer1 is essential for zebrafish development

Cited by 435 publications

References 10 publications

MicroRNAs and their regulatory roles in animals and plants

MicroRNAs and their regulatory roles in animals and plants

Tiny Actors, Great Roles: microRNAs in p53's Service

Characterization of Dicer-deficient murine embryonic stem cells

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