The MicroRNA Signature of Mouse Spermatozoa Is Substantially Modified During Epididymal Maturation1

Nixon, Brett; Stanger, Simone J.; Mihalas, Bettina P.; Reilly, Jackson N.; Anderson, Andy; Tyagi, Sonika; Holt, Janet E.; McLaughlin, Eileen A.

doi:10.1095/biolreprod.115.132209

Cited by 177 publications

(212 citation statements)

References 66 publications

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“…Furthermore, epididymosomes can deliver RNAs to immature sperm (Sharma et al 2016). It also has been confirmed that the sperm RNA profile is altered during the epididymal maturation in mice (Nixon et al 2015) and that epididymosomes can directly facilitate such changes (Reilly et al 2016). Therefore, in addition to the testes, the epididymis may be an important origin of the sperm RNA.…”

Section: Discussionmentioning

confidence: 86%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Section: Discussionmentioning

confidence: 86%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

View full text Add to dashboard Cite

show abstract

“…Alternatively, emerging evidence supports a direct influence of the environment on the epigenetic profile of spermatozoa. Environmental enrichment for 28 days, which accounts for the spermatogenic cycle of Mus musculus (Oakberg, 1956), could be altering the microRNA content of exosomes secreted by the epididymal epithelial cells which then fuse with the mature sperm in the caudal epididymis (Nixon et al, 2015a(Nixon et al, , 2015b. Environmental enrichment may be altering the dynamics of exosomal release and the microRNA content of these epididysomes, similar to its recently demonstrated impact on peripheral blood mononuclear cells (Pusic and Kraig, 2014;Pusic et al, 2016).…”

Section: Potential Mechanisms Mediating the Transgenerational Inheritmentioning

confidence: 87%

Paternal environmental enrichment transgenerationally alters affective behavioral and neuroendocrine phenotypes

Yeshurun

Short

Bredy

et al. 2017

Psychoneuroendocrinology

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1016/j.psyneuen.2016.11.013 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractRecent studies have demonstrated that paternal stress in rodents can result in modification of offspring behavior. Environmental enrichment, which enhances cognitive stimulation and physical activity, modifies various behaviors and reduces stress responses in adult rodents. We investigated the transgenerational influence of paternal environmental enrichment on offspring behavior and physiological stress response. Adult C57BL/6J male mice (F0) were exposed to either environmental enrichment or standard housing for four weeks and then pair-mated with naïve females. The F2 generation was generated using F1 male offspring. Male and female F1 and F2 offspring were tested for anxiety using the elevated-plus maze and large open field at 8 weeks of age. Depression-related behavior was assessed using the forced-swim test. Hypothalamic-pituitary-adrenal (HPA) axis function was determined by quantification of serum corticosterone and adrenocorticotropic hormone (ACTH) levels at baseline and after forced-swim stress. Paternal environmental enrichment was associated with increased body weights of male F1 and F2 offspring. There was no significant effect on F1 offspring anxiety and depression-related behaviors. There were no changes in anxietyrelated behaviors in the F2 offspring, however these mice displayed a reduced latency to immobility in the forced-swim test. Furthermore, F2 females had significantly higher serum corticosterone levels post-stress, but not ACTH. These results show that paternal environmental enrichment exerts a sex-specific transgenerational impact on the behavioral and physiological response to stress. Our findings have implications for the modelling of psychiatric disorders in rodents.

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“…Maturing sperm undergo an extensive reorganization of sncRNA content in the epididymal tract, epitomized by both gain and loss of hundreds of miRNAs (Nixon et al 2015). This RNA remodeling probably occurs via interaction with RNA-rich extracellular vesicles shed from the epididymal epithelium (epididymosomes) (Belleannee et al 2013, Reilly et al 2016, Sharma et al 2016.…”

Section: Small Non-coding Rnamentioning

confidence: 99%

Paternal epigenetic programming: evolving metabolic disease risk

Hur

Cropley

Suter

2017

Journal of Molecular Endocrinology

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Parental health or exposures can affect the lifetime health outcomes of offspring, independently of inherited genotypes. Such 'epigenetic' effects occur over a broad range of environmental stressors, including defects in parental metabolism. Although maternal metabolic effects are well documented, it has only recently been established that that there is also an independent paternal contribution to long-term metabolic health. Both paternal undernutrition and overnutrition can induce metabolic phenotypes in immediate offspring, and in some cases, the induced phenotype can affect multiple generations, implying inheritance of an acquired trait. The male lineage transmission of metabolic disease risk in these cases implicates a heritable factor carried by sperm. Sperm-based transmission provides a tractable system to interrogate heritable epigenetic factors influencing metabolism, and as detailed here, animal models of paternal programming have already provided some significant insights. Here, we review the evidence for paternal programming of metabolism in humans and animal models, and the available evidence on potential underlying mechanisms. Programming by paternal metabolism can be observed in multiple species across animal phyla, suggesting that this phenomenon may have a unique evolutionary significance.

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The MicroRNA Signature of Mouse Spermatozoa Is Substantially Modified During Epididymal Maturation1

Cited by 177 publications

References 66 publications

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Paternal environmental enrichment transgenerationally alters affective behavioral and neuroendocrine phenotypes

Paternal epigenetic programming: evolving metabolic disease risk

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