The microtubular cytoskeleton and chromosomes of unfertilized human oocytes aged in vitro

Eichenlaub-Ritter, Ursula; Stahl, A; Luciani, J. M.

doi:10.1007/bf01790094

Cited by 98 publications

(51 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several mechanisms have been proposed as being responsible for increasing the risk of these abnormalities with increased maternal age. Hormonal imbalances [125,126], abnormalities in follicular development due to the ageing of the somatic cells surrounding the oocyte [127,128] and impaired perifollicular microcirculation [129] have all been implicated as the possible mechanisms behind the effects of ageing on female fertility.…”

Section: Menopausementioning

confidence: 99%

Reproductive ageing in women

Djahanbakhch

Ezzati

Zosmer

2007

The Journal of Pathology

View full text Add to dashboard Cite

The traditional view in respect to female reproduction is that the number of oocytes at birth is fixed and continuously declines towards the point when no more oocytes are available after menopause. In this review we briefly discuss the embryonic development of female germ cells and ovarian follicles. The ontogeny of the hypothalamic-pituitary-gonadal axis is then discussed, with a focus on pubertal transition and normal ovulatory menstrual cycles during female adult life. Biochemical markers of menopausal transition are briefly examined. We also examine the effects of age on female fertility, the contribution of chromosomal abnormalities of the oocyte to the observed decline in female fertility with age and the possible biological basis for the occurrence of such abnormalities. Finally, we consider the effects of maternal age on obstetric complications and perinatal outcome. New data that have the potential to revolutionize our understanding of mammalian oogenesis and follicular formation, and of the female reproductive ageing process, are also briefly considered.

show abstract

Section: Menopausementioning

confidence: 99%

Reproductive ageing in women

Djahanbakhch

Ezzati

Zosmer

2007

The Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…49 Other authors 43 stated that unbalanced predivision and nondisjunction did not increase significantly with time in culture, but they found an increase in balanced predivision of chromatids also observed previously. 8 Some studies 52,53 revealed that the spindles of human oocytes were sensitive to prolonged in vitro culture and were morphologically altered. From published results, 54 it is conceivable that oocytes from older individuals could be sensitive to even a short culture time, but the morphological anomalies seen in the meiotic spindle suggest other mechanisms, for example, a suboptimal environment for oocyte maturation.…”

Section: Aneuploidy Ratementioning

confidence: 99%

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

et al. 2003

View full text Add to dashboard Cite

We used fluorescent in situ hybridisation (FISH) to detect nine chromosomes (1,13,15,16,17,18,21,22 and X) in 89 first Polar Bodies (1PBs), from in vitro matured oocytes discarded from IVF cycles. In 54 1PBs, we also analysed the corresponding oocyte in metaphase II (MII) to confirm the results; the other 35 1PBs were analysed alone as when preimplantation genetic diagnosis using 1PB (PGD-1PB) is performed. The frequency of aneuploid oocytes found was 47.5%; if the risk of aneuploidy for 23 chromosomes is estimated, the percentage rises to 57.2%. Missing chromosomes or chromatids found in 1PBs of 1PB/MII doublets were confirmed by MII results in 74.2%, indicating that only 25.8% of them were artefactual. Abnormalities observed in 1PBs were 55.8% whole-chromosome alterations and 44.2% chromatid anomalies. We observed a balanced predivision of chromatids for all chromosomes analysed. Differences between balanced predivision in 1PB and MII were statistically significant (Po0.0001, v 2 test); the 1PB was most affected. The mean abnormal segregation frequency for each chromosome was 0.89% (range 0.52-1.70%); so, each of the 23 chromosomes of an oocyte has a risk of 0.89% to be involved in aneuploidy. No significant differences were observed regarding age, type of abnormality (chromosome or chromatid alterations) or frequency of aneuploidy. Nine of the 35 patients (25.7%) whose 1PB and MII were studied presented abnormalities (extra chromosomes) that probably originated in early oogenesis. Analysis of 1PBs to select euploid oocytes could help patients of advanced age undergoing in vitro fertilization (IVF) treatment.

show abstract

“…Previous reports indicated the high incidence of spindle abnormality and chromosomal alignment abnormality in the oocytes cultured from 48 to 144 hours post insemination [20]. One study reported that unfertilized oocytes in IVF (18 to 22 hours post insemination) revealed normal morphology in 61% and abnormal morphology in 30%, with 9% showing no spindles [21].…”

Section: Discussionmentioning

confidence: 99%

Spindle and Chromosome Abnormality of Aged, Matured Oocytes in Human

et al. 2002

View full text Add to dashboard Cite

There are cases in which the 1-day-old unfertilized oocytes after IVF, or in vitro matured oocytes obtained from denuded immature oocytes after ICSI, are used clinically. We investigated the morphological change of metaphase II spindle and chromosomal alignment in those oocytes. The spindles and chromosome were stained using an anti-α-tubulin antibody and Hoechst 33258 respectively. One-day-old oocytes displayed significant increases in abnormalities o f s p i n d l e s t r u c t u r e ( 4 0 . 4 % v s . 1 7 . 6 % ) a n d chromosomal alignment (40.4% vs. 29.4%). Oocytes matured in vitro from MI and PI oocytes displayed spindle morphological abnormalities at the rates of 41.7% and 36.0% respectively, and chromosomal alignment abnormality at the rates of 45.8% and 44.0% respectively. These results suggest that 1-day-old oocytes and in vitro matured oocytes obtained from denuded immature oocytes after ICSI might have lowered developmental potential. More than 50% of those oocytes were determined to be unsuitable for use as gametes.

show abstract

The microtubular cytoskeleton and chromosomes of unfertilized human oocytes aged in vitro

Cited by 98 publications

References 17 publications

Reproductive ageing in women

Reproductive ageing in women

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

Spindle and Chromosome Abnormality of Aged, Matured Oocytes in Human

Contact Info

Product

Resources

About