The Mig protein of<i>Streptococcus dysgalactiae</i>inhibits bacterial internalization into bovine mammary gland epithelial cells

Song, Xin-Ming; Perez-Casal, José; Potter, Andrew

doi:10.1016/s0378-1097(03)00923-6

Cited by 14 publications

(7 citation statements)

References 16 publications

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“…dysgalactiae (GCS) is a pathogen frequently associated with clinical and subclinical bovine mastitis, a disease that causes major economic losses in the dairy industry (51,67). Virulence determinants have been identified for this pathogen, such as surface proteins which specifically interact with plasma or extracellular matrix proteins of the host, such as alpha-2-macroglobulin, plasminogen, albumin, fibrinogen, fibronectin, vitronectin, and collagen (30,35,46,64), and genes coding for proteins assumed to play a role in mastitis, such as the alpha-2-macroglobulin-, immunoglobulin G-, or immunoglobulin Abinding protein Mig (25,55); the alpha 2-macroglobulin-or immunoglobulin G-binding protein Mag (24); and a fibrinogen-binding M-like protein (65).…”

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confidence: 99%

“…Bovine GCS is known to grow well in mammary secretions, either during lactation or from dry animals (44,55), which may be necessary for survival and establishment in the specific environmental niche that is the bovine mammary gland. The results obtained in the present study by using the BioScreen suggest the environmental adaptability of the bovine GCS strains, which demonstrated the ability to grow fast in bovine blood serum and also, although with lower levels of growth, in the remaining tested media (THY and bovine milk serum).…”

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confidence: 99%

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Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

et al. 2011

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A custom-designed microarray containing 220 virulence genes of Streptococcus pyogenes (group A Streptococcus [GAS]) was used to test group C Streptococcus dysgalactiae subsp. dysgalactiae (GCS) field strains causing bovine mastitis and group C or group G Streptococcus dysgalactiae subsp. equisimilis (GCS/GGS) isolates from human infections, with the latter being used for comparative purposes, for the presence of virulence genes. All bovine and all human isolates carried a fraction of the 220 genes (23% and 39%, respectively). The virulence genes encoding streptolysin S, glyceraldehyde-3-phosphate dehydrogenase, the plasminogen-binding M-like protein PAM, and the collagen-like protein SclB were detected in the majority of both bovine and human isolates (94 to 100%). Virulence factors, usually carried by human beta-hemolytic streptococcal pathogens, such as streptokinase, laminin-binding protein, and the C5a peptidase precursor, were detected in all human isolates but not in bovine isolates. Additionally, GAS bacteriophage-associated virulence genes encoding superantigens, DNase, and/or streptodornase were detected in bovine isolates (72%) but not in the human isolates. Determinants located in non-bacteriophage-related mobile elements, such as the gene encoding R28, were detected in all bovine and human isolates. Several virulence genes, including genes of bacteriophage origin, were shown to be expressed by reverse transcriptase PCR (RT-PCR). Phylogenetic analysis of superantigen gene sequences revealed a high level (>98%) of identity among genes of bovine GCS, of the horse pathogen Streptococcus equi subsp. equi, and of the human pathogen GAS. Our findings indicate that alphahemolytic bovine GCS, an important mastitis pathogen and considered to be a nonhuman pathogen, carries important virulence factors responsible for virulence and pathogenesis in humans.

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Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

et al. 2011

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“…The bovine mammary epithelial cells (MAC-T cells) were obtained from the American Type Culture Collection (ATCC CRL-10274; Rockville, MD, USA) and cultured as indicated in [9].…”

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confidence: 99%

Milk-derived antimicrobial peptides to protect against Neonatal Diarrheal Disease: An alternative to antibiotics

Wilson¹,

Buchanan²,

Allan³

et al. 2012

Procedia in Vaccinology

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Neonatal Diarrheal Disease is responsible for significant economic losses to the livestock industries in Canada and around the world. Microbes responsible are diverse and include Escherichia coli, Salmonella, Rotavirus, Coronavirus and Cryptosporidia. While the use of antibiotics as a treatment for bacterial infections and as a prophylactic additive in feed has dramatically improved cattle production in recent decades, the increasing pressure to reduce or eliminate use of antibiotics in animals has caused the livestock industry to seek appropriate alternatives. Antimicrobial/Host Defense Peptides are natural compounds present on skin and in secretions in plants and animals that are microbicidal for bacteria, viruses, and parasites and they stimulate the immune system to combat infectious diseases. Our objective is to establish orally-obtained Host Defense Peptides (HDPs) as an alternative to antibiotics to protect against Neonatal Diarrheal Disease in calves. We devised a method to allow the cow udder to act as a factory to produce HDPs so that suckling calves will receive a continuous oral dose of HDPs over several weeks to protect them against neonatal diarrhea. We will use Adenovirus to deliver a gene coding for several HDPs in-frame into mammary epithelial cells. The epithelial cells will secrete the HDP protein into milk to be consumed by the suckling calves and trypsin in the calf gut will release the HDPs through cleavage. Thus, the novelty of this research lies not only in the proposed alternative to antibiotics to protect neonates against disease, but in the method by which we introduce the peptides to the suckling offspring.

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“…Bacterial adherence to A549 cells was characterized in 6-well cell culture plates (Corning) using a previously described method (Song et al 2004) with minor modifications. Following incubation with bacteria, host cells were washed 3 times with 0.1 molÁL -1 phosphate-buffered saline buffer (PBS) and lysed with a PBS buffer supplemented with 1% (m/v) saponin (Sigma) and 0.025% (m/v) trypsin.…”

Section: Bacterial Adherence Assaymentioning

confidence: 99%

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells

et al. 2008

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Streptococcus pneumoniae infection starts from the respiratory tract where interaction with host epithelial cells occurs. To gain more insights on pneumococcal pathogenesis, an oligonucleotide (oligo)-based microarray was used to investigate gene expression changes of one serotype 3 encapsulated pathogenic S. pneumoniae strain 82 and one unencapsulated avirulent S. pneumoniae strain R6 upon exposure to human lung epithelial cells (A549) for 1 and 3 h, respectively. We observed that genes associated with many functional categories were differentially regulated in strain 82, such as genes in pathogenesis, cell envelope, transcription, translation, transport, metabolism, and unknown functions. In contrast, few genes were changed in strain R6 except for genes in ribonucleotide biosynthesis and unknown functions. Quantitative real-time PCR analysis confirmed the microarray results for most of the genes tested. To further characterize functions of the selected genes, knockout mutants were constructed in strain R6. We demonstrated that 2 genetic loci, SP_2170 (AdcB, zinc ABC transporter) and SP_0157 (hypothetical protein), were involved in adherence to A549 cells. These data suggest that divergent gene expression changes occur in S. pneumoniae pathogenic and avirulent strains during interaction with human lung epithelial cells. Some of those genes are involved in pneumococcal pathogenesis.

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The Mig protein ofStreptococcus dysgalactiaeinhibits bacterial internalization into bovine mammary gland epithelial cells

Cited by 14 publications

References 16 publications

Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

Milk-derived antimicrobial peptides to protect against Neonatal Diarrheal Disease: An alternative to antibiotics

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells

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