We have studied 14 southern infrared dark clouds (IRDCs) using the data taken from the Millimetre Astronomy Legacy Team 90 GHz (MALT90) survey and the GLIMPSE and MIPSGAL mid-infrared survey of the inner Galaxy. The physical and chemical characteristics of the 14 IRDCs are investigated using N 2 H + (1-0), HNC(1-0), HCO + (1-0), and HCN(1-0) molecular lines. We find that the 14 IRDCs are in different evolutionary stages from the "starless" to the sources with an UCHII region. Three IRDCs are detected to have the star forming activities. The integrated intensity ratios I HCO + /HCN , I N2H + /HCN , and I HNC/HCN are all about 1.5, which is different from the previous measurements, suggesting that the integrated intensity ratios may be affected by the cloud environments. The integrated intensities of HNC, HCO + and HCN show a tight correlation for the 14 IRDCs, implying a close link to the chemical evolution of these three species in the IRDCs. The derived excitation temperature for each IRDC is less than 25 K. We estimate the abundances of the four molecules from 10 −11 to 10 −9 , and the average abundance ratios N HNC /N HCN = 1.47 ± 0.50, N HNC /N HCO + = 1.74 ± 0.22, N HCN /N HCO + = 1.21 ± 0.41.