1972
DOI: 10.1136/vr.91.4.79
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The minimum protective dose of the herpesvirus turkeys vaccine against Marek's disease

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Cited by 22 publications
(8 citation statements)
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“…We found that 1.5 PFU of cell-free HVT vaccine was required to induce viremia in 50% of antibodynegative line P chickens. This value correlates well with previous ID 50 data (Churchill and Baxendale, 1972;Patrascu et al, 1972;Purchase et al, 1972) and supports the validity of our in vitro assay procedure. We found only a negligible (9.7%) drop in titre of cell-associated vaccine after 4 hours at 4°C whereas these conditions produced a 22.7% drop in the titre of cell-free HVT vaccine; the drop in cell-free HVT titre was even more severe under conditions of continuous agitation (Table 1).…”
Section: Vaccine Titresupporting
confidence: 80%
“…We found that 1.5 PFU of cell-free HVT vaccine was required to induce viremia in 50% of antibodynegative line P chickens. This value correlates well with previous ID 50 data (Churchill and Baxendale, 1972;Patrascu et al, 1972;Purchase et al, 1972) and supports the validity of our in vitro assay procedure. We found only a negligible (9.7%) drop in titre of cell-associated vaccine after 4 hours at 4°C whereas these conditions produced a 22.7% drop in the titre of cell-free HVT vaccine; the drop in cell-free HVT titre was even more severe under conditions of continuous agitation (Table 1).…”
Section: Vaccine Titresupporting
confidence: 80%
“…The challenge times used in this study (14, 21 and 28 d.p.v. ) were selected to enable sampling of birds for vaccine virus measurement at several time-points prior to challenge, and are comparable with those used in previous studies on vaccinal protection (de Boer et al, 1981(de Boer et al, , 1987Purchase et al, 1972). Birds were kept for a further 53 days after the final challenge, and were monitored for morbidity and mortality.…”
Section: Methodsmentioning
confidence: 98%
“…Responses of chicks to MD vaccines, including CVI988 (de Boer et al, 1981(de Boer et al, , 1986(de Boer et al, , 1987 and HVT (Purchase et al, 1972;Basarab & Hall, 1976;Eidson et al, 1978), have previously been measured by viraemia assay to confirm replication of vaccine virus in the blood, by PD 50 assay to show immune protection against challenge virus, and by serological assay to demonstrate antibodies against vaccine virus. Our test quantifies CVI988 in chicks by measurement of viral DNA in feather tips.…”
Section: Correlation Of Protection With Cvi988 Level In Feathersmentioning
confidence: 99%
“…Following demostration of the efficacy of using lyphilized cell-free HVT as a vaccine against Marek's disease (MD) (Calnek et al, 1970;Churchill, 1971;Patrascu et al, 1972;Purchase et al, 1972;Zanella, 1971;Zanella ef a/., 1973;Zygraich ef a/., 1972), efforts have been made to improve the techniques of vaccine preparation, as well as to develop methods of assay in vitro and in vivo.…”
Section: Introductionmentioning
confidence: 99%