The minor salivary gland proteome in Sjögren’s syndrome

Hjelmervik, Trond Ove R.; Jönsson, Roland; Bolstad, Anne Isine

doi:10.1111/j.1601-0825.2009.01531.x

Cited by 34 publications

(19 citation statements)

References 56 publications

(87 reference statements)

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“…We observed down regulation of Ig J in all types of DES associated with non Sjogrens, primary and secondary to RA. Studies showed down regulation of Ig J chain in salivary gland tissue of primary SS cases using proteomic approach after treatment with the steroids [37], [38]. Further quantification in tear would help in ascertaining the role in inflammation seen in DES especially before and after treatment.…”

Section: Discussionmentioning

confidence: 99%

Lacrimal Proline Rich 4 (LPRR4) Protein in the Tear Fluid Is a Potential Biomarker of Dry Eye Syndrome

et al. 2012

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Dry eye syndrome (DES) is a complex, multifactorial, immune-associated disorder of the tear and ocular surface. DES with a high prevalence world over needs identification of potential biomarkers so as to understand not only the disease mechanism but also to identify drug targets. In this study we looked for differentially expressed proteins in tear samples of DES to arrive at characteristic biomarkers. As part of a prospective case-control study, tear specimen were collected using Schirmer strips from 129 dry eye cases and 73 age matched controls. 2D electrophoresis (2DE) and Differential gel electrophoresis (DIGE) was done to identify differentially expressed proteins. One of the differentially expressed protein in DES is lacrimal proline rich 4 protein (LPRR4). LPRR4 protein expression was quantified by enzyme immune sorbent assay (ELISA). LPRR4 was down regulated significantly in all types of dry eye cases, correlating with the disease severity as measured by clinical investigations. Further characterization of the protein is required to assess its therapeutic potential in DES.

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Section: Discussionmentioning

confidence: 99%

Lacrimal Proline Rich 4 (LPRR4) Protein in the Tear Fluid Is a Potential Biomarker of Dry Eye Syndrome

et al. 2012

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“…The salivary glands broadly express proteins that may be modified by PAD, including vimentin, enolase, and others [21–23]. Furthermore, the salivary glands constitutively express PAD2, and this enzyme may convert arginine residues into citrulline, which is the cornerstone of the citrullination process.…”

Section: Discussionmentioning

confidence: 99%

Posttranslational Protein Modification in the Salivary Glands of Sjögren’s Syndrome Patients

Herrera‐Esparza

Rodríguez-Rodríguez

Pérez-Pérez

et al. 2013

Autoimmune Diseases

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The present study investigated posttranslational reactions in the salivary glands of patients with Sjögren's syndrome. We analysed the biopsies of primary Sjögren's patients using immunohistochemistry and a tag-purified anticyclic citrullinated protein (CCP) antibody to detect citrullinated peptides, and the presence of peptidylarginine deiminase 2 (PAD2) was assessed simultaneously. The present work demonstrated the weak presence of the PAD2 enzyme in some normal salivary glands, although PAD2 expression was increased considerably in Sjögren's patients. The presence of citrullinated proteins was also detected in the salivary tissues of Sjögren's patients, which strongly supports the in situ posttranslational modification of proteins in this setting. Furthermore, the mutual expression of CCP and PAD2 suggests that this posttranslational modification is enzyme dependent. In conclusion, patients with Sjögren's syndrome expressed the catalytic machinery to produce posttranslational reactions that may result in autoantigen triggering.

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“…Проте-омный состав слюнных желез больных синдромом Шегре-на (СШ) характеризовался гиперэкспрессией таких бел-ков, как β-актин, α-дефенсин, кальмодулин, кальгранулин и кератин. Повышенный уровень последнего ассоцииро-вался с поражением ацинозных клеток, а содержание каль-модулина и кальгранулина отражало активность воспали-тельного процесса [76]. У пациентов с остеоартрозом (ОА) в хондроцитах хряща при помощи 2D-гель-электрофореза и MS было идентифицировано 20 белковых кандидатных маркеров заболевания.…”

Section: поиск биомаркеров ревматических заболеванийunclassified