The miR-130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN

Egawa, Hiroshi; Jingushi, Kentaro; Hirono, Takayuki; Ueda, Yuko; Kitae, Kaori; Nakata, Wataru; Fujita, Kazutoshi; Uemura, Mamoru; Nonomura, Norio; Tsujikawa, Kazutake

doi:10.1038/srep20574

Cited by 111 publications

(96 citation statements)

References 69 publications

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“…For instance, miR-23b could function as a tumor suppressor that present downregulation in stomach cancer (35) and bladder cancer (36). Whereas the miR-130 family have been identified as upregulated in many cancer types that representing putative oncogene (37,38). This pan-cancer wide meta-profiling analysis indicated that the comprehensive investigation of the database may help to illuminate the complicated relationship between miRNAs and cancers and develop more effective treatment strategies for cancers.…”

Section: Discussionmentioning

confidence: 99%

dbDEMC 2.0: updated database of differentially expressed miRNAs in human cancers

et al. 2016

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MicroRNAs (miRNAs) are often deregulated in cancer and are thought to play an important role in cancer development. Large amount of differentially expressed miRNAs have been identified in various cancers by using high-throughput methods. It is therefore quite important to make a comprehensive collection of these miRNAs and to decipher their roles in oncogenesis and tumor progression. In 2010, we presented the first release of dbDEMC, representing a database for collection of differentially expressed miRNAs in human cancers obtained from microarray data. Here we describe an update of the database. dbDEMC 2.0 documents 209 expression profiling data sets across 36 cancer types and 73 subtypes, and a total of 2224 differentially expressed miRNAs were identified. An easy-to-use web interface was constructed that allows users to make a quick search of the differentially expressed miRNAs in certain cancer types. In addition, a new function of ‘meta-profiling’ was added to view differential expression events according to user-defined miRNAs and cancer types. We expect this database to continue to serve as a valuable source for cancer investigation and potential clinical application related to miRNAs. dbDEMC 2.0 is freely available at http://www.picb.ac.cn/dbDEMC.

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Section: Discussionmentioning

confidence: 99%

dbDEMC 2.0: updated database of differentially expressed miRNAs in human cancers

et al. 2016

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“…Also, miR-301a is reported to promote colorectal cancer cell growth and invasion by directly targeting SOCS6 [37]. Meanwhile, Egawa and his colleagues showed that the miR-130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN [38]. The current study focused on the functions of miR-301a and its molecular mechanisms in MM.…”

Section: Discussionmentioning

confidence: 99%

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

Cui

Li²,

Lv³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Although microRNA-301a has been reported to function as an oncogene in many human cancers, the roles of miR-301a in malignant melanoma (MM) is unclear. The present study aims to investigate the functional roles of miR-301a in MM and its possible molecular mechanisms. Methods: Quantitative real-time PCR (qRT-PCR) assay was performed to detect the expression of miR-301a in MM tissues, and analyze its correlation with metastasis and prognosis of MM patients. In vitro, miR-301a was ectopically expressed using overexpression and knock-down strategies, and the effects of miR-301a expression on growth, apoptosis, migration, invasion and chemosensitivity of MM cells were further investigated. Furthermore, the potential and functional target gene was identified by luciferase reporter, qRT-PCR, Western blot assays. Results: We showed that the expression of miR-301a was significantly upregulated in MM tissues, and upregulation of miR-301a correlated with metastasis and poor prognosis of MM patients. Transfection of miR-301a/inhibitor significantly inhibited growth, colony formation, migration, invasion and enhanced apoptosis and chemosensitivity in MM cells, while transfection of miR-301a/mimic could induce the inverse effects on phenotypes of MM cells. Luciferase reporter, qRT-PCR and Western blot assays showed that phosphatase and tensin homolog (PTEN) was a direct and functional target of miR-301a. It was also observed that the Akt and FAK signaling pathways were involved in miR-301/PTEN-promoting MM progression. Conclusion: Taken together, our study suggests that miR-301a may be used as a potential therapeutic target in the treatment of human MM.

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“…Recently, miR-130b was demonstrated to have tumor suppressive and cancer-promoting properties depending on the tumor type. miR-130b is highly expressed in melanoma (9), gastric (10), bladder (11) and colorectal cancer (12), esophageal squamous cell carcinoma (13) and glioma (14), but significantly and poorly expressed in papillary thyroid carcinomas (15), endometrial cancer (16), pituitary adenomas (17) and pancreatic cancer (18). Previously, miR-130b was observed to be overexpressed in glioma tissues, while the suppression of the expression of miR-130b via peroxisome proliferator-activated receptor-γ (PPAR-γ) inhibited the proliferation and invasive activity of glioma (14).…”

Section: Introductionmentioning

confidence: 99%

miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD

Xiao

Yin

et al. 2017

Oncology Reports

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Abstract. MicroRNAs are short non-coding RNAs that play important roles in gliomas. However, the role of miR-130b in glioma remains unclear. In the present study, miR-130b expression was upregulated in glioma tissues and cell lines. Kaplan-Meier analysis indicated that the upregulation of miR-130b expression correlated with poor prognoses in glioma patients. Multivariate analysis demonstrated that this upregulation and a high-grade classification were independent factors that both predicted poor outcomes for glioma patients. Dual-luciferase assays identified that the cylindromatosis (CYLD) gene is a direct target of miR-130b. Functional studies demonstrated that a miR-130b mimic significantly promoted the growth and invasion of glioma cells, while also inhibiting apoptosis via selective targeting of CYLD, which was enhanced by CYLD-targeted siRNA. In contrast, a miR-130b inhibitor suppressed these biological behaviors, and this inhibition was reversed by CYLD-targeted siRNA. These data revealed that miR-130b could act as a novel potential diagnostic biomarker for glioma, while also demonstrating the importance of miR-130b in the cell proliferation and progression of glioma, indicating that it may serve as a useful therapeutic target for glioma.

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The miR-130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN

Cited by 111 publications

References 69 publications

dbDEMC 2.0: updated database of differentially expressed miRNAs in human cancers

dbDEMC 2.0: updated database of differentially expressed miRNAs in human cancers

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

miR-130b regulates the proliferation, invasion and apoptosis of glioma cells via targeting of CYLD

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