The miR-155 regulates cytokines expression by SOSC1 signal pathways of fish in vitro and in vivo

Tang, Xueyuan; Fu, Jin-Dong; Tan, Xukai; Shi, Yunfeng; Ye, Jiawei; Guan, Wanting; Shi, Yifu; Xu, Min-Jun

doi:10.1016/j.fsi.2020.07.042

Cited by 19 publications

(12 citation statements)

References 62 publications

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“…In the present research, the expressions of IL‐1, TNF‐α, IFN‐γ, and TLR2 were affected—accompanied by the changes in expression of miR‐155—which indicated that the cytokine genes may participate in the process of immune regulation of miR‐155 in fishes, as our previous research showed (Tang et al. 2020). Other miRNAs have also been reported as relevant with the regulation of the above genes.…”

Section: Discussionsupporting

confidence: 79%

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MicroRNAs and Related Cytokine Factors Quickly Respond in the Immune Response of Channel Catfish to Lipopolysaccharides and β‐Glucan Stimulation

Tang

Shi

et al. 2021

J Aqua Anim Hlth

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MicroRNAs (miRNAs) are well‐known as powerful regulators of gene expression, with their potential to serve for immunology widely researched in mammals and birds but rarely in fishes. To better understand fish immunology behavior, we herein investigated nine immune‐related miRNAs that were reported in other animals, as well as five related cytokine factors and lysozyme (LZM) in the liver, anterior kidney, and spleen of Channel Catfish Ictalurus punctatus after being stimulated by lipopolysaccharides (LPS) and β‐glucan. We also predicated the potential targets of these miRNAs via bioinformatics and further investigated nine of them via quantitative real‐time PCR. Results showed that expressions of the nine miRNAs were quickly changed in varying extent after stimulation by LPS, especially for miR‐122, miR‐142a, miR‐155, and miR‐223, which were significantly changed in spleen, and the same occurred for the LZM and three cytokine factors TNF‐α, IFN‐γ and TLR2. Compared with LPS, although most of the miRNAs and the cytokine genes were also affected by β‐glucan, the extent of the effect was weak. Bioinformatics analysis revealed many immune‐related targets of the miRNAs, with some of them reported by previous studies. For the nine investigated target genes, seven targets (77.8%) were significantly upregulated after the stimulation of LPS. It therefore can be inferred that the immune‐related miRNAs, LZM, and cytokine factors elicited quick immune responses of Channel Catfish to LPS stimulation as in other animals, but the regulation mechanism of miRNAs might be complex and diverse. This research will contribute to a better understanding will support further immunology research in fishes.

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Section: Discussionsupporting

confidence: 79%

“…In our previous research, the relationship among miR‐155, cytokine expression, and the SOSC1 signal pathways in fishes were reported (Tang et al. 2020). However, the functions of most of the miRNAs still remain unknown in Channel Catfish.…”

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confidence: 97%

MicroRNAs and Related Cytokine Factors Quickly Respond in the Immune Response of Channel Catfish to Lipopolysaccharides and β‐Glucan Stimulation

Tang

Shi

et al. 2021

J Aqua Anim Hlth

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“…Notably, BCO2 expression was apparently inhibited and promoted by miR-330 mimics and inhibitor when compared with the control. Agomir, a cholesterol-conjugated double-stranded RNA molecule, has been widely used in several fish species to verify the relationship between miRNAs and their target genes in vivo [33][34][35], which was also adopted to further confirm the effect of miR-330 overexpression on BCO2. As expected, we found that miR-330 expression level was significantly enhanced in the dorsal skin, tail fin and liver, while the BCO2 gene was marked downregulation.…”

Section: Discussionmentioning

confidence: 99%

miR-330 targeting BCO2 is involved in carotenoid metabolism to regulate skin pigmentation in rainbow trout (Oncorhynchus mykiss)

Zhao

Huang

et al. 2023

BMC Genomics

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Background MicroRNAs (miRNAs) play a critical role in regulating skin pigmentation. As a key economic trait, skin color directly affects the market value of rainbow trout (Oncorhynchus mykiss), however, the regulatory mechanism of most miRNAs in fish skin color is still unclear. Results In this study, the full-length cDNA sequence of β-carotene oxygenase 2 (BCO2, a key regulator of carotenoid metabolism) from the rainbow trout was obtained using rapid-amplification of cDNA ends (RACE) technology, and qRT-PCR was used to investigate the differential expression of miR-330 and BCO2 in 14 developmental stages and 13 tissues between wild-type rainbow trout (WTrt) and yellow mutant rainbow trout (YMrt). Additionally, the function of miR-330 was verified by overexpression and silencing in vitro and in vivo. The results showed that the complete cDNA sequence of BCO2 was 2057 bp with a 1707 bp ORF, encoding a 568 amino acid protein having a molecular weight of 64.07 kD. Sequence alignment revealed that higher conservation of BCO2 protein amongst fishes than amongst other vertebrates, which was further confirmed by phylogenetic analysis. The analysis of spatial and temporal expression patterns suggested that BCO2 and miR-330 were abundantly expressed from fertilized-stage to multi-cell as well as in the dorsal and ventral skin of WTrt and YMrt, and their expression patterns were opposite in most of the same periods and tissues. In vitro, luciferase reporter assay confirmed that BCO2 was a direct target of miR-330, and transfection of miR-330 mimics into rainbow trout liver cells resulted in a decrease in the expression of BCO2; conversely, miR-330 inhibitor had the opposite effect to the miR-330 mimics. In vivo, miR-330 agomir significantly decreased BCO2 expression in dorsal skin, tail fin, and liver. Furthermore, overexpression of miR-330 could suppress cell proliferation and induce apoptosis. Conclusion Our results showed that miR-330 is involved in the regulation of skin pigmentation in rainbow trout by targeting BCO2 and shows its promise as a potential molecular target to assist the selection of rainbow trout with better skin color patterns.

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“…Among the five known DEmiRs (miR-132-3p, miR-194a, miR-155, miR-203b-5p, and miR-146), cal-miR-155 showed the highest up-regulation compared to the other four miRNAs, indicating that it might play an essential role in defending against NNV infection in host cell lines. Previous studies have confirmed that miR-155 is related to the immune reaction and has antiviral effects, such as regulating cytokine expression of Cyprinus carpi , and exhibiting anti-CyHV-3 activity in common carp brain (CCB) [ 48 , 53 , 54 ]. According to the results after cell infection at different time points, the expression of cal-miR-155 markedly decreased after 24 hpi, indicating that the function of cal-miR-155 might act in early-stages of the NNV infection.…”

Section: Discussionmentioning

confidence: 99%

Cromileptes altivelis microRNA Transcriptome Analysis upon Nervous Necrosis Virus (NNV) Infection and the Effect of cal-miR-155 on Cells Apoptosis and Virus Replication

Cao

Liu

et al. 2022

Viruses

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MicroRNAs (miRNAs) could regulate various biological processes. Nervous necrosis virus (NNV) is one of the primary germs of the Humpback grouper (Cromileptes altivelis), a commercial fish of great importance for Asian aquaculture. However, there is limited available information on the host-virus interactions of C. altivelis. miRNAs have been shown to play key roles in the host response to infection by a variety of pathogens. To better understand the regulatory mechanism of miRNAs, we constructed miRNA transcriptomes and identified immune-related miRNAs of C. altivelis spleen in response to NNV infection. Reads from the three libraries were mapped onto the Danio rerio reference genome. As a result, a total of 942 mature miRNAs were determined, with 266 known miRNAs and 676 novel miRNAs. Among them, thirty-two differentially expressed miRNAs (DEmiRs) were identified compared to the PBS control. These DEmiRs were targeted on 895 genes, respectively, by using miRanda v3.3a. Then, 14 DEmiRs were validated by qRT-PCR and showed consistency with those obtained from high-throughput sequencing. In order to study the relationship between viral infection and host miRNA, a cell line from C. altivelis brain (CAB) was used to examine the expressions of five known DEmiRs (miR-132-3p, miR-194a, miR-155, miR-203b-5p, and miR-146) during NNV infection. The results showed that one miRNA, cal-miRNA-155, displayed significantly increased expression in response to the virus infection. Subsequently, it was proved that overexpression of cal-miR-155 enhanced cell apoptosis with or without NNV infection and inhibited virus replication in CAB cells. Oppositely, the cal-miRNA-155 inhibitor markedly suppressed apoptosis in CAB cells. The results of the apoptosis-related genes mRNA expression also showed the regulation of cal-miR-155 on the apoptosis process in CAB cells. These findings verify that miR-155 might exert a function as a pro-apoptotic factor in reply to NNV stimulation in CAB cells and help us further study the molecular mechanisms of the pathogenesis of NNV in C. altivelis.

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The miR-155 regulates cytokines expression by SOSC1 signal pathways of fish in vitro and in vivo

Cited by 19 publications

References 62 publications

MicroRNAs and Related Cytokine Factors Quickly Respond in the Immune Response of Channel Catfish to Lipopolysaccharides and β‐Glucan Stimulation

MicroRNAs and Related Cytokine Factors Quickly Respond in the Immune Response of Channel Catfish to Lipopolysaccharides and β‐Glucan Stimulation

miR-330 targeting BCO2 is involved in carotenoid metabolism to regulate skin pigmentation in rainbow trout (Oncorhynchus mykiss)

Cromileptes altivelis microRNA Transcriptome Analysis upon Nervous Necrosis Virus (NNV) Infection and the Effect of cal-miR-155 on Cells Apoptosis and Virus Replication

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