2018
DOI: 10.1186/s13046-018-0679-5
|View full text |Cite
|
Sign up to set email alerts
|

The miR-181 family promotes cell cycle by targeting CTDSPL, a phosphatase-like tumor suppressor in uveal melanoma

Abstract: BackgroundMicroRNAs (miRNAs) have been shown to function in many different cellular processes, including proliferation, apoptosis, differentiation and development. miR-181a, -181b, -181c and -181d are miR-181 members of the family, which has been rarely studied, especially uveal melanoma.MethodsThe expression level of miR-181 family in human uveal melanoma cell lines was measured via real-time PCR (RT-PCR). The function of miR-181 on cell cycle was detected through Flow Cytometry assay. Microarray assay and Bi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
58
0
4

Year Published

2018
2018
2024
2024

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 53 publications
(63 citation statements)
references
References 45 publications
1
58
0
4
Order By: Relevance
“…Oncogenic miRNAs in UM include the miR-181 family, comprising four miRNAs (miR-181a/b/c/d), which are transcribed from different gene loci. In UM, it was found that all miR-181 family members were highly homologous and able to regulate the effector gene CTDSPL, a phosphatase-like tumour suppressor gene that dephosphorylates Rb1 and regulates the RNA polymerase II transcription machinery [51]. All members of the miR-181 family were significantly overexpressed in UM and cell lines (including 92.1 cells); however, miR-181b was highlighted as the most effective regulator of the downstream gene CTDSPL.…”
Section: Mirna As Oncogenes In Ummentioning
confidence: 99%
See 1 more Smart Citation
“…Oncogenic miRNAs in UM include the miR-181 family, comprising four miRNAs (miR-181a/b/c/d), which are transcribed from different gene loci. In UM, it was found that all miR-181 family members were highly homologous and able to regulate the effector gene CTDSPL, a phosphatase-like tumour suppressor gene that dephosphorylates Rb1 and regulates the RNA polymerase II transcription machinery [51]. All members of the miR-181 family were significantly overexpressed in UM and cell lines (including 92.1 cells); however, miR-181b was highlighted as the most effective regulator of the downstream gene CTDSPL.…”
Section: Mirna As Oncogenes In Ummentioning
confidence: 99%
“…Aberrant microRNA (miRNA) expression, frequently observed in a myriad of cancers, is also reported in UM. The studies undertaken in UM include those investigating (a) the functional roles of specific miRNAs in cell lines and tumour samples [50,51] and (b) the prognostic and diagnostic use of miRNAs, individually and in networks, from not only tissue samples but also from plasma and circulating exosomes [52,53], as well as larger data-mining in silico studies often utilizing and extrapolating the power of The Cancer Genome Analysis (TCGA) study [54][55][56]. However, at present, there is no clear consensus as to which of the identified miRNAs should be targeted for use either as diagnostic biomarkers or as potential druggable targets.…”
Section: Introductionmentioning
confidence: 99%
“…The molecular signature of the proteins in the normal basal/parabasal epithelium was low nuclear/cytoplasmic expression of RBSP3 and high nuclear with high/ moderate cytoplasmic expressions of LIMD1 and CDC25A.Etiological factors (HPV, tobacco) did not influence the above molecular signature, with the results being comparable to previous reports in H&N epithelium [ 8 , 9 ] and other tissues [ 18 , 19 ]. RBSP3, acts as a tumour suppressor phosphatase, that is important for regulating RNA polymerase II transcriptional machinery, attenuating BMP signaling and dephosphorylating pRB to block G1/ S cell cycle transition [ 20 , 21 ]. RBSP3 shows alteration in different cancers including uveal melanoma, lung cancer, cervical cancer, etc.…”
Section: Discussionmentioning
confidence: 99%
“…RBSP3 shows alteration in different cancers including uveal melanoma, lung cancer, cervical cancer, etc. [ 20 , 22 , 23 ]. Differential expression of RBSP3 as observed by us, might be necessary for cellular proliferation and differentiation.…”
Section: Discussionmentioning
confidence: 99%
“…In UM, it causes suppression of p53 gene expression and increases the expression of the cell-adhesion protein LASP1 and GST-pi protein—an enzyme involved in cell detoxification—protecting tumor cells from cytotoxic drugs. Zhang et al analyzed the action of the miR-181 members family (miR-181a, miR-181b, miR-181c), finding that their expression was increased in UM cell lines [ 40 ]. In particular, miR-181b was reported to inhibit the expression of the tumor-suppressing phosphatase enzyme (CTDSPL) that dephosphorylates tumor suppressor Rb; thus, this miRNA inhibits the role of Rb in increasing cell proliferation [ 40 ].…”
Section: Micrornas In Ummentioning
confidence: 99%