The miR-200 family: multiple effects on gliomas

Peng, Lilei; Fu, Jie; Yang, Ming

doi:10.2147/cmar.s160945

Cited by 22 publications

(19 citation statements)

References 54 publications

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“…41 The tumor suppressive role of miR-200 family is closely associated with the glinoma initiation, progression and metastasis. 42 These findings suggested the opposite function of miR-200a-3p and other miR-200 family members in cancers. In the present study, we found that miR-200a-3p was significantly upregulated in ovarian cancer tissues and cell lines.…”

Section: Discussionmentioning

confidence: 99%

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

Shi¹,

Yang²,

Zhang³

et al. 2019

OTT

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Background: Increasing evidence has revealed that the aberrant expression of microRNAs (miRNAs) plays vital roles in the development and progression of ovarian cancer. MiR-200a-3p was found to act as an oncogene in a variety of cancers, however, the expression and function of miR-200a-3p in ovarian cancer has not been characterized. Materials and methods: The expression of miR-200a-3p in ovarian cancer tissues and cell lines was detected by the RT-qPCR. The influence of miR-200a-3p on the growth of ovarian cancer cells was determined with the Cell Counting Kit-8 assay, colony formation and cell invasion assay. The binding of miR-200a-3p with the 3ʹ-untranslated region (UTR) of PDCH9 was detected by luciferase reporter assay. The expression of PCDH9 was investigated by RT-qPCR and Western blot analysis. Results: miR-200a-3p was up-regulated in ovarian cancer tissues and cell lines. Highly expressed miR-200a-3p was significantly associated with the tumor size, tumor metastasis and TNM stage. Overexpression of miR-200a-3p markedly promoted the proliferation, colony formation and invasion of ovarian cancer cells. Functional study uncovered that miR-200a-3p bound the 3ʹ-untranslated region (UTR) of PCDH9 and decreased the expression of PCDH9 in ovarian cancer cells. The expression of miR-200a-3p in ovarian cancer tissues was significantly negatively correlated with that of PCDH9. Restored PCDH9 inhibited the promoting effect of miR-200a-3p on the proliferation of ovarian cancer cells. Conclusion: Our results suggested the potential oncogenic function of miR-200a-3p via modulating PCDH9 in ovarian cancer.

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Section: Discussionmentioning

confidence: 99%

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

Shi¹,

Yang²,

Zhang³

et al. 2019

OTT

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“…[9]. The miR-200 family members (miR-200a, miR-200b, miR-200c, miR-141, and miR-429) [10] could inhibite epithelial-mesenchymal transition (EMT) by targeting E-cadherin, ZEB1 or ZEB2 [11]. Interestingly, It was found ZEB1could bind to the promoter regions ofmiR-200b/200a/429 or miR-200c/141, suggesting that miR-200b could target inhibition of ZEB1 and control cell migration, invasion, and EMT [12].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-200b reduced ZEB1 to inhibit cell proliferation and migration in human gastric cancer

Chen

Liu

Wang

et al. 2020

Preprint

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BACKGROUND : Gastric cancer (GC) is one of the most common malignant cancers, with high morbidity and mortality rates worldwide. The present study was to explore whether miR-200b is a tumor suppressor in GC and to unveil the potential mechanisms. METHODS: Levels of c-Myc, Cyclin D1, MMP-3 and MMP-9 expression were detected respectively by qRT-PCR and Western blot assay. BrdU proliferation assay, Cell cycle analysis, Wound-healing and Transwell assays were used to study the role of miR-200b with inhibitor, mimics or ZEB1-RNAi in TGF-β1-treated SGC-7901/DDP cells. The xenograft model with nude mice was established to unveil the role of miR-200b in vivo . RESULTS : Compared with the paracancerous tissues, miR-200b was decreased in GC patients and SGC-7901/DDP cells. Lower level of miR-200b induced by its inhibitor promoted TGF-β1-treated SGC-7901/DDP cells proliferation and migration, and increased the levels of c-Myc, Cyclin D1, MMP-3, MMP-9, β-catenin and APC. Interestingly, miR-200b mimics and ZEB1-RNAi were able to reduce the proliferation and migration of TGF-β1-induced SGC-7901/DDP cells as well as their levels of c-Myc, Cyclin D1, MMP-3 MMP-9, β-catenin and APC. In addition, ZEB1 was indeed the potential target of miR-200b identified by dual luciferase reporter gene assay. Xenograft model also suggested that over-expression of miR-200b suppressed the growth of tumor in vivo. CONCLUSION : Taken together, our findings suggest that miR-200b be likely to play an important role in activating TGF-β1-induced SGC-7901/DDP cells and perform as a tumor suppressor by targeting ZEB1 in GC. What’s more, miR-200b may modulate Wnt/β-catenin signaling pathway in TGF-β1-induced SGC-7901/DDP cells.

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“…Diffuse low-grade (WHO grade II) and III intermediate-grade gliomas are called lowergrade gliomas (LGGs) [4]. Because of their rapid growth and highly remarkable aggressiveness, a subset of these LGGs will develop into glioblastoma (WHO grade IV) within several months [5]. Glioblastoma (GBM) is the most common and malignant glioma, which is characterized by poor clinical prognosis and the survival time rarely exceeds 14 months [6].…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, despite some remarkable achievements, the median survival time of glioma patients has not been signi cantly prolonged in the past decades [5]. There is still much room for research in the underlying pathogenesis, the multistep progression of glioma, and how to improve survival.…”

Section: Introductionmentioning

confidence: 99%

Identification of collagen genes related to immune infiltration and epithelial-mesenchymal transition in glioma

Yin

Tan

Xin

et al. 2020

Preprint

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Background: Gliomas account for the majority of fatal primary brain tumors, and there is much room for research in the underlying pathogenesis, the multistep progression of glioma, and how to improve survival. In our study, we aimed to identify potential biomarkers or therapeutic targets of glioma and study the mechanism underlying the tumor progression. Methods: We downloaded the microarray datasets (GSE43378 and GSE7696) from the Gene Expression Omnibus (GEO) database. Then, we used weighted gene co-expression network analysis (WGCNA) to screen potential biomarkers or therapeutic targets related to the tumor progression. ESTIMATE (Estimation of STromal and Immune cells in MAlignant Tumors using Expression data) algorithm and TIMER (Tumor Immune Estimation Resource) database were used to analyze the correlation between the selected genes and the tumor microenvironment. Real-time reverse transcription polymerase chain reaction was used to measure the selected gene. Transwell and wound healing assay were used to measure the cell migration and invasion capacity.Results: We identified specific module genes that were positively correlated with the WHO grade but negatively correlated with OS of glioma. Importantly, we identified that 6 collagen genes (COL1A1, COL1A2, COL3A1, COL4A1, COL4A2, and COL5A2) could regulate the immunosuppressive microenvironment of glioma. Moreover, we found that these collagen genes were significantly involved in the epithelial-mesenchymal transition (EMT) process of glioma. Finally, taking COL5A2 as a further research object, the results showed that knockdown of COL5A2 significantly inhibited the migration and invasion of SHG44 and A172 cells. Conclusions: In summary, our study demonstrated that collagen genes play an important role in regulating the immunosuppressive microenvironment and EMT process of glioma and could serve as potential therapeutic targets for glioma management.

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The miR-200 family: multiple effects on gliomas

Cited by 22 publications

References 54 publications

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

MicroRNA-200b reduced ZEB1 to inhibit cell proliferation and migration in human gastric cancer

Identification of collagen genes related to immune infiltration and epithelial-mesenchymal transition in glioma

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