The miR-506-Induced Epithelial–Mesenchymal Transition is Involved in Poor Prognosis for Patients with Gastric Cancer

Sakimura, Shotaro; Sugimachi, Keizō; Kurashige, Junji; Ueda, Masami; Hirata, Hidenari; Nambara, Sho; Komatsu, Hisateru; Saito, Tomoko; Takano, Yuki; Uchi, Ryutaro; Sakimura, Etsuko; Shinden, Yoshiaki; Iguchi, Tomohiro; Eguchi, Hidetoshi; Oba, Yugo; Hoka, Sumio; Mimori, Koshi

doi:10.1245/s10434-015-4418-2

Cited by 23 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-506, located on chromosome x, has been reported to be involved in diverse biological behaviors depending on different target genes. It has been shown that miR-506 expression is downregulated in several types of cancers such as gastric (18), cervical (14), ovarian (21) and lung cancer (22), suggesting that miR-506 plays an important role in tumorigenesis and tumor progression. Yang et al reported that miR-506 is downregulated in clear cell renal cell carcinoma and inhibits cell growth and metastasis via targeting forkhead box Q1 (FLOT1) (23).…”

Section: Discussionmentioning

confidence: 99%

“…MicroRNAs (miRNAs) are small (19-24 nucleotides), single-stranded, non-coding RNA molecules (18)(19)(20)(21)(22)(23)(24) that usually lead to gene silencing by binding to complementary sequences in the three prime untranslated regions (3'UTRs) of target messenger RNA (mRNA) transcripts (6)(7)(8). miRNAs are involved in various biological processes, such as cell division, cell cycle, differentiation, proliferation development and apoptosis (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

“…miR-506, a recently discovered miRNA, has been reported to function as a tumor suppressor in human cancers including cervical cancer (14), breast cancer (15), epithelial ovarian cancer (16), oral squamous cell carcinoma (17), and gastric cancer (18). However, the role of miR-506 in glioma and the mechanisms underlying glioma carcinogenesis remain unclear.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

miR-506 functions as a tumor suppressor in glioma by targeting STAT3

et al. 2015

View full text Add to dashboard Cite

MicroRNA-506 (miR-506) has been reported to act as a tumor suppressive or an oncogenic miRNA in different types of tumors. However, the roles and underlying molecular mechanism of miR-506 in glioma remain unclear. In the present study, we performed quantitative PCR to investigate the level of miR-506 in 36 pairs of glioma tumor and matched adjacent tissues, and found that miR-506 was downregulated in the glioma tumors compared to the expression in the adjacent normal tissues. Furthermore, a functional assay found that ectopic expression of miR-506 in glioma cells markedly suppressed cell proliferation, colony formation, migration and invasion, and suppressed tumor growth in vivo. Moreover, signal transducer and activator of transcription 3 (STAT3) was identified as a direct target of miR-506. Western blot assay showed that overexpression of miR-506 not only induced changes in STAT3 expression but also altered expression of its downstream genes, including, Bcl2, cyclin D1 and matrix metalloproteinase 2 (MMP-2), in the human glioma cells. In addition, STAT3 mRNA expression was increased in the glioma tissues, and was inversely correlated with miR-506. Importantly, overexpression of STAT3 in glioma cells attenuated the suppressive effects of miR-506 on cell proliferation, colony formation, migration and invasion. These results showed that miR-506 functions as a tumor suppressor in glioma by targeting STAT3, suggesting that miR-506 may serve as a potential target in the treatment of human glioma.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

miR-506 functions as a tumor suppressor in glioma by targeting STAT3

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Furthermore, miR-506 suppressed the metastasis of breast cancer cells by targeting the IQ motif containing GTPase-activating protein 1 (29). In gastric cancer, miR-506 inhibited EMT by targeting ETS1 (37) or SNAI2 (38). miR-506 also suppressed the proliferation and invasion of gastric cancer cells by targeting Yap1 (39).…”

Section: Discussionmentioning

confidence: 99%

Suppression of forkhead box Q1 by microRNA-506 represses the proliferation and epithelial-mesenchymal transition of cervical cancer cells

Zhang

Yan

et al. 2016

Oncology Reports

View full text Add to dashboard Cite

Abstract. MicroRNAs (miRNAs) play a pivotal role in cancer progression and development, representing novel therapeutic tools for cancer therapy. Forkhead box Q1 (FOXQ1) functions as an oncogene in various cancer types. However, the functional significance of FOXQ1 in cervical cancer remains unknown. In this study, we investigated the biological function of FOXQ1 in cervical cancer and tested whether or not FOXQ1 can be targeted and regulated by specific miRNAs. We found that FOXQ1 was highly expressed in cervical cancer cell lines. Knockdown of FOXQ1 by small interfering RNA (siRNA) significantly suppressed the proliferation and epithelial-mesenchymal transition (EMT) of cervical cancer cells. FOXQ1 was predicted as a target gene of microRNA-506 (miR-506), and this prediction was validated by dual-luciferase reporter assay. Quantitative real-time PCR and western blot analyses demonstrated that mRNA and protein expression was negatively regulated by miR-506. The expression of miR-506 was downregulated in cervical cancer tissues, and miR-506 expression was inversely correlated with FOXQ1 expression in cervical cancer. The overexpression of miR-506 dramatically suppressed the proliferation and EMT of cervical cancer cells that mimicked the suppression of FOXO1 siRNA. Furthermore, the restoration of FOXQ1 expression significantly reversed the inhibitory effect of miR-506. Overall, our study demonstrated that miR-506 inhibited the proliferation and EMT of cervical cancer cells by targeting FOXQ1 and provided evidence that the miR-506/FOXQ1 axis plays an important role in the pathogenesis of cervical cancer, representing potential molecular targets for the development of anticancer agents for cervical cancer treatment.

show abstract

“…MiR-506 was identified as a member of the miR-506-514 cluster, which includes seven distinct miRNAs: miR-506, -507, -508, -509, -510, -513 and miR-514 [14,15]. Besides, its abnormal expression was detected to be associated with multiple benign diseases and malignant carcinomas [16][17][18][19][20]. Wen et al reported that miR-506 exerted its anti-proliferative function by directly targeting Gli3 in human CC [21].…”

mentioning

confidence: 99%

miR-506 suppresses cervical cancer cell proliferation both in vitro and in vivo

Gong¹,

Chen²,

Zhao³

et al. 2018

neo

View full text Add to dashboard Cite

Cervical cancer (CC) is one of the most common gynecological malignancies in women worldwide. Recently increasing evidences indicate aberrant expression of miR-506 was reported to be associated with a variety of tumors. The aimof this study was to evaluate the potential role of miR-506 in CC and verify its effect on the regulation of ABCC4. The expression of miR-506 in cervical cancer tissues and HeLa and C33A cell lines was examined using quantitative Real-time PCR. MTT assay and animals studies were use to examine the effects of miR-506 on cervical cancer proliferation. Luciferase reporter and western blot were used to confirm miR-506 could regulate ABCC4. We found that miR-506 was significantly downregulated in human CC cell lines (HeLa and C33A) and clinical CC specimens as compared with matched cell lines and adjacent normal tissues, while the expression level of ABCC4 was higher in tumor tissues than it in adjacent normal tissues. We also revealed that up-regulated expression of miR-506 could inhibit CC cells proliferation both in vitro and in vivo. Moreover, ABCC4 was identified as a direct target of miR-506 and the inverse relationship between them was also observed. In summary, our finding suggests that miR-506 acts an important role in suppressing CC cell proliferation and suppresses the expression of ABCC4 by directly targeting its 3'-UTR. miR-506 may represent a novel therapeutic target of microRNA-mediated suppression of cell proliferation in CC, but the role of the miR-506/ABCC4 axis in CC progression needs further study.

show abstract

The miR-506-Induced Epithelial–Mesenchymal Transition is Involved in Poor Prognosis for Patients with Gastric Cancer

Abstract: The EMT was directly suppressed by miR-506, and its low expression was an independent prognostic factor in gastric cancer patients. The data indicated that miR-506 may act as a tumor suppressor and could be a novel therapeutic agent.

Cited by 23 publications

References 26 publications

miR-506 functions as a tumor suppressor in glioma by targeting STAT3

miR-506 functions as a tumor suppressor in glioma by targeting STAT3

Suppression of forkhead box Q1 by microRNA-506 represses the proliferation and epithelial-mesenchymal transition of cervical cancer cells

miR-506 suppresses cervical cancer cell proliferation both in vitro and in vivo

Contact Info

Product

Resources

About