The miR164-TaNAC14 module regulates root development and abiotic-stress tolerance in wheat seedlings

Chi, Qing; Du, Linying; Wen, Ming; NIU, Ruo-yu; Wu, Baowei; Guo, Lijian; Ma, Meng; LIU, Xiang-li; Zhao, Huixian

doi:10.1016/j.jia.2022.08.016

Cited by 12 publications

(9 citation statements)

References 52 publications

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“…As a highly conserved class of miRNAs in plants, the microRNA164 (miR164) family has been widely studied in various plant species during the past (almost) 20 years [21][22][23][24][25][26][27][28]. Similarly to other miRNAs, miR164 functions in plant growth and development by forming regulation modules with its target genes, mainly NAC (NAM, ATAF, and CUC) transcription factors and a few less broadly conserved target genes containing a sequence complementary to miR164 with few mismatches.…”

Section: Discussionmentioning

confidence: 99%

“…Overexpressing a miR164-targeted NAC transcription factor NAC21/22 negatively regulates stripe rust resistance in wheat [34] and the overexpression of miR164-targeted NAC transcription factor genes has negative effects on drought resistance in rice at the reproduction stage [18]. Similarly, in wheat seedlings, tae-miR164 inhibits root development and reduces drought and salinity tolerance by downregulating the expression of TaNAC14 [21]. In this study, we found that miR164 affected plant responses to UV by generating OS-miR164a-overexpressing (OE164) and -silencing (MIM164) perennial ryegrass, more specifically, by having a negative effect on UV tolerance.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA164 Affects Plant Responses to UV Radiation in Perennial Ryegrass

Xu,

Huang,

et al. 2024

Plants

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Increasing the ultraviolet radiation (UV) level, particularly UV-B due to damage to the stratospheric ozone layer by human activities, has huge negative effects on plant and animal metabolism. As a widely grown cool-season forage grass and turfgrass in the world, perennial ryegrass (Lolium perenne) is UV-B-sensitive. To study the effects of miR164, a highly conserved microRNA in plants, on perennial ryegrass under UV stress, both OsmiR164a overexpression (OE164) and target mimicry (MIM164) transgenic perennial ryegrass plants were generated using agrobacterium-mediated transformation, and UV-B treatment (~600 μw cm−2) of 7 days was imposed. Morphological and physiological analysis showed that the miR164 gene affected perennial ryegrass UV tolerance negatively, demonstrated by the more scorching leaves, higher leaf electrolyte leakage, and lower relative water content in OE164 than the WT and MIM164 plants after UV stress. The increased UV sensitivity could be partially due to the reduction in antioxidative capacity and the accumulation of anthocyanins. This study indicated the potential of targeting miR164 and/or its targeted genes for the genetic manipulation of UV responses in forage grasses/turfgrasses; further research to reveal the molecular mechanism underlying how miR164 affects plant UV responses is needed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA164 Affects Plant Responses to UV Radiation in Perennial Ryegrass

Xu,

Huang,

et al. 2024

Plants

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“…The miR164 family is well-known to play important roles in various abiotic stresses. For instance, the miR164-TaNAC14 module regulates drought tolerance in wheat seedlings, with tae-miR164 reducing drought and salinity tolerance by downregulating the expression of TaNAC14 [12]. Fang et al [13] also suggest that the conserved miR164-targeted NAC genes may be negative regulators of drought tolerance in rice.…”

Section: Mir164 Plays a Role In Regulating Plant Adaptation To Changi...mentioning

confidence: 99%

“…The microRNA164 (miR164) family is another highly conserved class of miRNAs in plants [11]. In Arabidopsis, the miR164 family consists of three members (miR164a, miR164b, miR164c), which mediate five NAC (NAM, ATAF1/2, and CUC2) transcription factor genes; in rice, it contains six members of miR164a/b/f, miR164c, miR164d, and miR164e, which mainly target six NAC transcription factors (OMTN1-6, Oryza miR164-targeted NAC), and some non-NAC genes, such as PSK5 (phytosulfokines precursor gene) and PM27 (seed maturation protein gene) [12][13][14][15]. These target genes are mainly involved in plant development, senescence-mediated cell death, and biotic/abiotic stresses [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…These target genes are mainly involved in plant development, senescence-mediated cell death, and biotic/abiotic stresses [13,14]. For example, a number of studies have reported the regulation of miR164 by different environmental stimuli, particularly abiotic stresses, such as salt, cold, high temperature, drought, and heavy metals [12,[16][17][18]. However, other than one study that showed the expression of miR164b was down-regulated under the treatment of high light (1500 µmol m −2 •s −1 ) in Phyllostachys edulis [17], no study has reported the relationship between miR164 and the plant response to diverse light intensity.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA164 Regulates Perennial Ryegrass (Lolium perenne L.) Adaptation to Changing Light Intensity

Zhang,

Huang,

Liu

et al. 2024

Agronomy

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Plants especially need to adapt to all different light environments (shade, high light, etc.) due to the essential role of light in plant life. Either shade or high-light microenvironmental conditions are common for cool-season turfgrasses, such as perennial ryegrass (Lolium perenne L.). In order to study how a plant highly conserves microRNA, miR164-affected perennial ryegrass were studied under different light intensities. OsmiR164a-overexpression (OE164), target mimicry OsmiR164a (MIM164), and CRES-T (chimeric repressor gene-silencing technology) OsNAC60 (NAC60) transgenic plants and wild-type (WT) plants were evaluated in both field (shade and full sun) and growth chamber conditions (low, medium, and high PAR at 100, 400, and 1200 µmol s−1 m−2). Morphological and physiological analysis showed miR164 could fine-tune perennial ryegrass adaptation to changing light intensity, possibly via the regulation of target genes, such as NAC60. Overall, OE164 and NAC60 plants were similar to each other and more sensitive to high light, particularly under the field condition, demonstrated by smaller size and much poorer grass quality; MIM164 performed more like WT plants than either the OE164 or NAC60 plants. This study indicates the potential of genetic manipulation of miR164 and/or its targeted genes for turfgrass adaptation to changing light environments, and future research to further investigate the molecular mechanism beneath would be warranted.

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Endoplasmic reticulum stress-responsive microRNAs are involved in the regulation of abiotic stresses in wheat

Chen

2023

Plant Cell Rep

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The miR164-TaNAC14 module regulates root development and abiotic-stress tolerance in wheat seedlings

Cited by 12 publications

References 52 publications

MicroRNA164 Affects Plant Responses to UV Radiation in Perennial Ryegrass

MicroRNA164 Affects Plant Responses to UV Radiation in Perennial Ryegrass

MicroRNA164 Regulates Perennial Ryegrass (Lolium perenne L.) Adaptation to Changing Light Intensity

Endoplasmic reticulum stress-responsive microRNAs are involved in the regulation of abiotic stresses in wheat

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