The miR399-<i>CsUBC24</i> Module Regulates Reproductive Development and Male Fertility in Citrus

Wang, Rong; Fang, Yan-Ni; Wu, Xiaomeng; Qing, Mei; Li, Chaochao; Xie, Kai‐Dong; Deng, Xiuxin; Guo, Wen‐Wu

doi:10.1104/pp.20.00129

Cited by 45 publications

(32 citation statements)

References 73 publications

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“…The male fertility of 337S is believed to be the result of tae-miR2275-CAF1 and tae-miR1127a-SMARCA3L3 miRNA—target coregulation [ 60 ]. Recently, the role of the MIR399 family in regulating reproductive development and male fertility in plants was reported [ 61 ]. Wang et al found that miR399a has the highest abundance in floral organs of citrus and verified that CsUBC24 (PHO2) is the target gene for the miR399a-mediated suppression of expression.…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis , rice, and maize, the PHOSPHATE2 gene ( PHO2 ) is targeted by miR399 [ 81 , 82 , 83 ], and PHO2 negatively regulates inorganic phosphate (Pi) uptake and translocation by downregulating the expression of PSI-related genes [ 84 ]. Wang et al also provided evidence that Pi uptake and translocation in citrus might be impeded by miR399a.1-STTM [ 61 ]. Alterations in lipid metabolism, resulting in phospholipid degradation and galactolipid and sulfolipid synthesis, could increase intracellular Pi availability under Pi depletion [ 85 ].…”

Section: Discussionmentioning

confidence: 99%

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Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Sun

Xiong

Wang

et al. 2021

IJMS

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A photoperiod- and thermo-sensitive genic male sterile (PTGMS) line is the basic material for two-hybrid rice and is an important genetic breeding resource. Peiai64S (PA64S) is an important germplasm resource of PTGMS rice, and it has been applied to two-line hybrid rice systems in China. Pollen fertility in PA64S is regulated by the temperature and photoperiod, but the mechanism of the fertility transition is unclear. In this study, we obtained the male fertile plant PA64S(F) and the male sterile plant PA64S(S) by controlling different temperatures under long light conditions and used the male fertile and sterile plants to investigate the role of microRNAs (miRNAs) in regulating male fertility in rice. We performed the small RNA library sequencing of anthers from PA64S(S) and PA64S(F). A total of 196 miRNAs were identified—166 known miRNAs among 27 miRNA families and 30 novel miRNAs. In the transcriptome analysis, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of differentially expressed genes revealed significant enrichment in the synthesis and metabolism of fatty acids and some secondary metabolism pathways such as fatty acid metabolism and phenylalanine metabolism. With a comprehensive analysis of miRNA, transcriptome, and degradome sequencing, we identified that 13 pairs of miRNA/target genes regulated male fertility in rice by responding to temperature change, among which the miR156, miR5488, and miR399 affect the male fertility of PA64S by influencing SPLs, the lignin synthesis of anther walls, and the flavonoid metabolism pathway. The results provide a new understanding of PTGMS rice, which will help us better understand the potential regulatory mechanisms of male sterility in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Sun

Xiong

Wang

et al. 2021

IJMS

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“…The same result was found in this study; gma-miR398a and gma-miR398b were induced by HT and upregulated in the flower bud of HT-sensitive CMS-based F 1 (Table S1, Figure 3). miR399 is another conserved miRNA and plays a crucial role in regulating both the Pi homeostasis and reproductive development of the plant [46,47]. In this study, four members of miR399 (a, b, c, and h) were found to be lowly expressed in the flower bud of HT-tolerant and HT-sensitive CMS-based F 1 under NT, and their expression patterns were similar to those of gma-miR398a and gma-miR398b after HT stress (Table S1, Figure 3).…”

Section: Heat-responsive Mirnas Involved In the Regulation Of Male Fementioning

confidence: 99%

“…In this study, four members of miR399 (a, b, c, and h) were found to be lowly expressed in the flower bud of HT-tolerant and HT-sensitive CMS-based F 1 under NT, and their expression patterns were similar to those of gma-miR398a and gma-miR398b after HT stress (Table S1, Figure 3). Previous studies have demonstrated that miR399-PHO2/UBC24 modules affect flowering time in response to ambient temperature changes and male fertility in Arabidopsis and Citrus [47,48], respectively. gma-miR399a was negatively correlated with GmIPT1-4 under HT (Figure 4), and GmIPT1-4 (Glyma.10G036800) was found to be highly expressed in the flower of soybean (Phytozome v12.1, https://phytozome.jgi.doe.gov/pz/portal.html# accessed on 14 February 2021), suggesting that the gma-miR399a/GmIPT1-4 module might mediate the fertility regulation of soybean CMS-based F 1 under HT stress.…”

Section: Heat-responsive Mirnas Involved In the Regulation Of Male Fementioning

confidence: 99%

Heat-Responsive miRNAs Participate in the Regulation of Male Fertility Stability in Soybean CMS-Based F1 under High Temperature Stress

Ding

Guo

Zhang

et al. 2021

IJMS

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MicroRNAs (miRNAs), a class of noncoding small RNAs (sRNAs), are widely involved in the response to high temperature (HT) stress at both the seedling and flowering stages. To dissect the roles of miRNAs in regulating male fertility in soybean cytoplasmic male sterility (CMS)-based F1 under HT, sRNA sequencing was performed using flower buds from HT-tolerant and HT-sensitive CMS-based F1 combinations (NF1 and YF1, respectively). A total of 554 known miRNAs, 59 new members of known miRNAs, 712 novel miRNAs, and 1145 target genes of 580 differentially expressed miRNAs (DEMs) were identified under normal temperature and HT conditions. Further integrated analysis of sRNA and transcriptome sequencing found that 21 DEMs and 15 differentially expressed target genes, such as gma-miR397a/Laccase 2, gma-miR399a/Inorganic phosphate transporter 1-4, and gma-miR4413a/PPR proteins, mitochondrial-like, were negatively regulated under HT stress. Furthermore, all members of the gma-miR156 family were suppressed by HT stress in both NF1 and YF1, but were highly expressed in YF1 under HT condition. The negative correlation between gma-miR156b and its target gene squamosa promoter-binding protein-like 2b was confirmed by expression analysis, and overexpression of gma-miR156b in Arabidopsis led to male sterility under HT stress. With these results, we proposed that miRNAs play an important role in the regulation of male fertility stability in soybean CMS-based F1 under HT stress.

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“…miR399 has been shown to regulate plant reproductive development. The miR399-UBC24 module regulates SEPALLATA MADS box transcription factor genes (SEPs) and ICE1 protein level to effect floral organ development in Citrus (R. Wang et al, 2020). The miR399-UBC24 module is also involved in plant other process such as sugar metabolism (Y.…”

Section: Introductionmentioning

confidence: 99%

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation

Peng

Tian

et al. 2021

Preprint

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Although the regulation in Pi homeostasis of miR399 have been studied in various plants, its molecular mechanisms in response to freezing stress are still elusive. In this work, we found that the expression of tae-miR399 and its target gene TaUBC24 in tillering nodes of strong cold resistance winter wheat cultivar Dongnongdongmai1 (Dn1) was significantly altered subjected to severe winter. tae-miR399 and its target gene TaUBC24 were also responsive to short-term freezing stress in tillering nodes of Dn1 seedlings. TaUBC24 physically interacted with TaICE1. Ehanced freezing tolerance was observed in overexpressing tae-miR399 Arabidopsis lines. Under freezing stress, overexpressing tae-miR399 decreased the expression of AtUBC24 to increase the expression of genes in CBF signaling pathway, Pi translocation pathway and starch metablism, including AtCBFs, AtCOR47, AtCOR413IM, AtPHT1;4, AtAPLs and AtBAMs, inhibit the degradation of AtICE1 and AtPHO1, and promote the activities of SOD, POD and CAT. These findings indicated that the increased freezing tolerance was dependent upon elevating CBF signaling pathway, phosphorus utilization efficiency, starch degradation, accumulation of soluble sugar and ability of ROS scavenge. These results will aid our understanding of molecular mechanism of how miR399-UBC24 module plays a cardinal role in regulating plant freezing stress tolerance through mediating the downstream pathways.

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The miR399-CsUBC24 Module Regulates Reproductive Development and Male Fertility in Citrus

Cited by 45 publications

References 73 publications

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Heat-Responsive miRNAs Participate in the Regulation of Male Fertility Stability in Soybean CMS-Based F1 under High Temperature Stress

miR399-UBC24 module enhances freezing tolerance through regulating CBF signaling pathway and starch degradation

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