The miRNA Profile of Platelets Stored in a Blood Bank and Its Relation to Cellular Damage from Storage

Pontes, Thaís Brilhante; Moreira-Nunes, Caroline Aquino; Maués, Jersey Heitor da Silva; Lamarão, Letícia Martins; Lemos, José Alexandre Rodrigues de; Montenegro, Raquel Carvalho; Burbano, Rommel Rodrı́guez

doi:10.1371/journal.pone.0129399

Cited by 36 publications

(43 citation statements)

References 46 publications

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“…As the miRNA post‐transcriptional control pathway is active in platelets, the effects of post‐collection processing and storage on the miRNA profile of PCs are of interest. It has been suggested that specific miRNA expression patterns can be used to monitor cellular damage related to PSL . Studies looking at miRNAs during platelet storage have shown high levels of miRNAs associated with apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…A few publications have focused on miRNA in stored platelets, showing differences in the regulation of miRNAs during storage and describing the effects of post‐collection processing methods like PI . It has even been suggested that specific miRNA patterns could predict the quality of stored PCs and that manipulation of the miRNA gene regulation pathway could lead to more effective storage of platelets . Research on miRNAs in stored PCs could contribute to better quality and prolonged shelf life of stored platelets …”

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Pathogen inactivation with amotosalen plus UVA illumination minimally impacts microRNA expression in platelets during storage under standard blood banking conditions

et al. 2019

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BACKGROUND: To reduce the risk of transfusion transmission infection, nucleic acid targeted methods have been developed to inactivate pathogens in PCs. miRNAs have been shown to play an important role in platelet function, and changes in the abundance of specific miRNAs during storage have been observed, as have perturbation effects related to pathogen inactivation (PI) methods. The aim of this work was to investigate the effects of PI on selected miRNAs during storage.STUDY DESIGN AND METHODS: Using a pool and split strategy, 3 identical buffy coat PC units were generated from a pool of 24 whole blood donors. Each unit received a different treatment: 1) Untreated platelet control in platelet additive solution (C-PAS); 2) Amotosalen-UVA-treated platelets in PAS (PI-PAS); and 3) untreated platelets in donor plasma (U-PL). PCs were stored for 7 days under standard blood banking conditions. Standard platelet quality control (QC) parameters and 25 selected miRNAs were analyzed.RESULTS: During the 7-day storage period, differences were found in several QC parameters relating to PI treatment and storage in plasma, but overall the three treatments were comparable. Out of 25 miRNA tested changes in regulation of 5 miRNA in PI-PAS and 3 miRNA U-PL where detected compared to C-PAS. A statistically significant difference was observed in down regulations miR-96-5p on Days 2 and 4, 61.9% and 61.8%, respectively, in the PI-PAS treatment. CONCLUSION:Amotosalen-UVA treatment does not significantly alter the miRNA profile of platelet concentrates generated and stored using standard blood banking conditions. P latelets play a central role in hemostasis and represent an integral part of transfusion medicine. Platelet concentrates (PCs) are normally stored at room temperature for a maximum of 5 to 7 days, depending on country-specific regulations. 1,2 One of two main reasons for this limited storage time is the potential for bacterial growth during storage. 3,4 The risk of transfusion-transmitted bacterial infections (TTBIs) due to contaminated PCs is persistent despite improved donor selection protocols, phlebotomy techniques, and PC bacterial screening. Life threatening septic transfusion reactions (STRs) related to platelet transfusions still occur. 4,5 The currently available bacterial detection assays are not sufficient to prevent TTBI. 6 The rate of STRs due to PC transfusion has likely been underestimated, as highlighted in a recent 7-year retrospective study of PC transfusions using passive and active surveillance. 7 The second reason for the relatively short shelf life of stored platelets is platelet storage lesion (PSL). PSL is a collective term for a variety of factors that describe the deterioration of platelet quality during storage. 8,9 The onset and acceleration of PSL during storage is likely to affect platelet post transfusion recovery and survival. 10,11 The onset and accumulation of PSL is related to the activation of or damage to the platelets as result of their preparation or storage conditions, and can eventual...

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Section: Discussionmentioning

confidence: 99%

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Pathogen inactivation with amotosalen plus UVA illumination minimally impacts microRNA expression in platelets during storage under standard blood banking conditions

et al. 2019

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“…That said, there are downfalls of analyzing the miRNA composition of whole blood as compared to serum, plasma or exosomes. Whole blood contains not only miRNAs from the target tissues, but also miRNAs from each individual cellular components of blood including erythrocytes [19], leukocytes [20] and platelets [21]. The miRNAs derived from the cellular components of blood provide background noise, making it more difficult to identify changes in placental-derived miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of circulating miRNAs during late pregnancy in the mare

et al. 2017

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MicroRNAs (miRNAs) are small, non-coding RNAs which are produced throughout the body. Individual tissues tend to have a specific expression profile and excrete many of these miRNAs into circulation. These circulating miRNAs may be diagnostically valuable biomarkers for assessing the presence of disease while minimizing invasive testing. In women, numerous circulating miRNAs have been identified which change significantly during pregnancy-related complications (e.g. chorioamnionitis, eclampsia, recurrent pregnancy loss); however, no prior work has been done in this area in the horse. To identify pregnancy-specific miRNAs, we collected serial whole blood samples in pregnant mares at 8, 9, 10 m of gestation and post-partum, as well as from non-pregnant (diestrous) mares. In total, we evaluated a panel of 178 miRNAs using qPCR, eventually identifying five miRNAs of interest. One miRNA (miR-374b) was differentially regulated through late gestation and four miRNAs (miR-454, miR-133b, miR-486-5p and miR-204b) were differentially regulated between the pregnant and non-pregnant samples. We were able to identify putative targets for the differentially regulated miRNAs using two separate target prediction programs, miRDB and Ingenuity Pathway Analysis. The targets for the miRNAs differentially regulated during pregnancy were predicted to be involved in signaling pathways such as the STAT3 pathway and PI3/AKT signaling pathway, as well as more endocrine-based pathways, including the GnRH, prolactin and insulin signaling pathways. In summary, this study provides novel information about the changes occurring in circulating miRNAs during normal pregnancy, as well as attempting to predict the biological effects induced by these miRNAs.

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“…Some of these pro apoptosis miRNA are further upregulated during the storage period and some are downregulated . One publication suggests using specific miRNA to monitor the cellular damage related to the PSL . A research group led by Dr. Provost has studied the effects of Intercept treatment on platelet transcriptome (miRNA and mRNA).…”

Section: Microrna Analysis and Platelet Storagementioning

confidence: 99%

New strategies to understand platelet storage lesion

Árnason

Sigurjónsson

2017

ISBT Science Series

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Modern health care is dependent on the banking and transfusion of platelet concentrates. Platelets, however, can pose a problem for stock management at blood banks due to their limited storage time. In most countries, platelets can be stored from 3 to 7 days and due limited storage time up to 30% of all platelet concentrates are discarded without ever being used for clinical transfusion. The main reasons for this limited storage time are increased risk of bacterial contamination, due to the storage conditions at 22°C, and a formation of a condition termed platelet storage lesion (PSL) that decreases the quality of the platelets and makes them less efficient for clinical use as the storage prolongs. Increased understanding of PSL formation and how it can be combated is important to increase the quality of platelets during storage, in turn making them more efficient for clinical use. There are several methods used to detect formation of PSL, including analysing expression of surface markers on platelets using flow cytometry, analysing function of platelets using light transmission aggregometry and release of cytokines and growth factors using, for example ELISA. However, those methods focus more on studying the consequence of PSL instead of the cause of PSL. In recent years, several laboratories, including ours, have been using novel ways to further try to understand the formation of PSL. These include, for example analysing changes in proteomics, miRNA and metabolomics in platelets during storage. In this short overview, we will review how novel methods have been used to shed new lights on the formation of PSL.

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The miRNA Profile of Platelets Stored in a Blood Bank and Its Relation to Cellular Damage from Storage

Cited by 36 publications

References 46 publications

Pathogen inactivation with amotosalen plus UVA illumination minimally impacts microRNA expression in platelets during storage under standard blood banking conditions

Pathogen inactivation with amotosalen plus UVA illumination minimally impacts microRNA expression in platelets during storage under standard blood banking conditions

Evaluation of circulating miRNAs during late pregnancy in the mare

New strategies to understand platelet storage lesion

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