The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae

Gross, Roy; Guzmán, Carlos A.; Sebaihia, Mohammed; Santos, Vítor A. P. Martins dos; Pieper, Dietmar H.; Koebnik, Ralf; Lechner, Melanie; Bartels, Daniela; Buhrmester, Jens; Choudhuri, Jomuna V.; Ebensen, Thomas; Gaigalat, Lars; Herrmann, Stefanie M.; Khachane, Amit N.; Larisch, Christof; Link, Stefanie; Linke, Burkhard; Meyer, Patrick; Mormann, Sascha; Nakunst, Diana; Rückert, Christian; Schneiker-Bekel, Susanne; Schulze, Kai; Vorhölter, Frank Jörg; Yevsa, Tetyana; Engle, Jacquelyn T.; Goldman, William E.; Pühler, Alfred; Göbel, Ulf B.; Goesmann, Alexander; Blöcker, Helmut; Kaiser, Olaf; Martínez‐Arias, Rosa

doi:10.1186/1471-2164-9-449

Cited by 84 publications

(107 citation statements)

References 53 publications

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“…strain P51 (van der Meer et al, 1991) and in Ralstonia sp. strain PS12 (Beil et al, 1999;Müller et al, 2003) are carried on transposons, or are localized on genomic islands, as in Bordetella petrii (Gross et al, 2008). Whether the upper pathway genes of strain GJ31 belong to its core genome or have been recruited by horizontal gene transfer remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome

et al. 2009

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Pseudomonas putida GJ31 has been reported to grow on chlorobenzene using a meta-cleavage pathway with chlorocatechol 2,3-dioxygenase (CbzE) as a key enzyme. The CbzE-encoding gene was found to be localized on the 180 kb plasmid pKW1 in a cbzTEXGS cluster, which is flanked by transposases and encodes only a partial (chloro)catechol meta-cleavage pathway comprising ferredoxin reductase, chlorocatechol 2,3-dioxygenase, an unknown protein, 2-hydroxymuconic semialdehyde dehydrogenase and glutathione S-transferase. Downstream of cbzTEXGS are located cbzJ, encoding a novel type of 2-hydroxypent-2,4-dienoate hydratase, and a transposon region highly similar to Tn5501. Upstream of cbzTEXGS, traNEOFG transfer genes were found. The search for gene clusters possibly completing the (chloro)catechol metabolic pathway of GJ31 revealed the presence of two additional catabolic gene clusters on pKW1. The mhpRBCDFETP cluster encodes enzymes for the dissimilation of 2,3-dihydroxyphenylpropionate in a novel arrangement characterized by the absence of a gene encoding 3-(3-hydroxyphenyl)propionate monooxygenase and the presence of a GntR-type regulator, whereas the nahINLOMKJ cluster encodes part of the naphthalene metabolic pathway. Transcription studies supported their possible involvement in chlorobenzene degradation. The upper pathway cluster, comprising genes encoding a chlorobenzene dioxygenase and a chlorobenzene dihydrodiol dehydrogenase, was localized on the chromosome. A high level of transcription in response to chlorobenzene revealed it to be crucial for chlorobenzene degradation. The chlorobenzene degradation pathway in strain GJ31 is thus a mosaic encoded by four gene clusters.

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Section: Discussionmentioning

confidence: 99%

Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome

et al. 2009

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“…Only four species, B. petrii (von Wintzingerode et al, 2001) and the three novel species mentioned above, were isolated from the environment. However, whole genome analysis verified that B. petrii has pathogenic genes in its genome (Gross et al, 2008). Interestingly, B. petrii has been isolated from clinical samples since the initial finding by von Wintzingerode et al (2001) (e.g., Fry et al, 2005;Le Coustumier et al, 2011;Stark et al, 2007).…”

Section: -7 Bordetellamentioning

confidence: 99%

“…In general, pathogenic Bordetella species are able to grow at temperatures around 37∞C and are fastidious, which is likely due to host-parasite interactions. Based on the whole genome sequence of B. petrii, Gross et al (2008) suggested that this bacterium might grow in a variety of environments because it has remarkable metabolic versatility. Most pathogenic species of the genus Bordetella lack metabolic versatility, so they are host-restricted pathogens.…”

Section: -7 Bordetellamentioning

confidence: 99%

Polyphasic insights into the microbiomes of the Takamatsuzuka Tumulus and Kitora Tumulus

Sugiyama¹,

Kiyuna²,

Nishijima³

et al. 2017

J. Gen. Appl. Microbiol.

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Microbial outbreaks and related biodeterioration problems have affected the 1300-year-old multicolor (polychrome) mural paintings of the special historic sites Takamatsuzuka Tumulus (TT) and Kitora Tumulus (KT). Those of TT are designated as a national treasure. The microbiomes of these tumuli, both located in Asuka village, Nara, Japan, are critically reviewed as the central subject of this report. Using culture-dependent methods (conventional isolation and cultivation), we conducted polyphasic studies of the these microbial communities and identified the major microbial colonizers (Fusarium spp., Trichoderma spp., Penicillium spp., dark Acremonium spp., novel Candida yeast spp., Bacillus spp., Ochrobactrum spp., Stenotrophomonas tumulicola, and a few actinobacterial genera) and noteworthy microbial members (Kendrickiella phycomyces, Cephalotrichum verrucisporum (∫ ∫ ∫ ∫ ∫Doratomyces verrucisporus), Sagenomella striatispora, Sagenomella griseoviridis, two novel Cladophialophora spp., Burgoa anomala, one novel species Prototheca tumulicola, five novel Gluconacetobacter spp., three novel Bordetella spp., and one novel genus and species Krasilnikoviella muralis) involved in the biodeterioration of mural paintings, plaster walls, and stone chamber interiors. In addition, we generated microbial community data from TT and KT samples using cultureindependent methods (molecular biological methods, including PCR-DGGE, clone libraries, and pyrosequence analysis). These data are comprehensively presented, in contrast to those derived from culture-dependent methods. Furthermore, the microbial communities detected using both methods are analytically compared, and, as a result, the complementary roles of these methods and approaches are highlighted. In related contexts, knowledge of similar biodeterioration problems affecting other prehistoric cave paintings, mainly at Lascaux in France and Altamira in Spain, are referred to and commented upon. Based on substrate preferences (or ecological grouping) and mapping (plotting detection sites of isolates), we speculate on the possible origins and invasion routes whereby the major microbial colonizers invaded the TT stone chamber interior. Finally, concluding remarks, lessons, and future perspectives based on our microbiological surveys of these ancient tumuli, and similar treasures outside of Japan, are briefly presented. A list of the microbial taxa that have been identified and fully or briefly described by us as known and novel taxa for TT and KT isolates since 2008 is presented in Supplementary Materials. 64SUGIYAMA et al.

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“…Gly-CCC and PaVRFPA04GI Gly-CCC that contains many multiantibiotics resistance genes has been named as GI6 [9] . The two GIs, namely PAO1GI-1 and PA14GI-6, associated with tRNA Gly-CCC gene have been determined in P. aeruginosa PAO1 and UCBPP-PA14 [10] .…”

Section: A Two Genomic Islands Were Integrated Into 5' and 3'mentioning

confidence: 99%

Structure Analysis of Genomic Islands Flanked by 5’ and 3’ End of the tRNAGly-CCC Gene in Delftia Acidovorans SPH-1

Su¹,

Peng²,

Zha³

et al. 2015

Proceedings of the International Conference on Chemical, Material and Food Engineering

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The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae

Cited by 84 publications

References 53 publications

Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome

Degradation of chloroaromatics by Pseudomonas putida GJ31: assembled route for chlorobenzene degradation encoded by clusters on plasmid pKW1 and the chromosome

Polyphasic insights into the microbiomes of the Takamatsuzuka Tumulus and Kitora Tumulus

Structure Analysis of Genomic Islands Flanked by 5’ and 3’ End of the tRNAGly-CCC Gene in Delftia Acidovorans SPH-1

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