The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus

Zhang, Ying; Liu, Chao; Wu, Bingbing; Li, Liansheng; Li, Wei; Li, Yuan

doi:10.1038/s41467-021-25227-w

Cited by 34 publications

(23 citation statements)

References 46 publications

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“…This cellular location of BmPMFBP1 is consistent with observations of PMFBP1’s cytoplasmic location in the round spermatids in the mouse [ 27 ]. In mature spermatozoa, mammalian PMFBP1 is a component of the sperm head-to-tail coupling apparatus assembly formed with interacting proteins CENTILEIN and SUN5 [ 39 ], and so is specifically localized to the fossa region of the sperm nucleus during sperm head elongation and differentiation [ 27 ]. However, if BmPMFBP1 similarly interacts with other sperm proteins, our fusion protein assay would be unlikely to detect it.…”

Section: Resultsmentioning

confidence: 99%

BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

Yang

Chen

et al. 2022

PLoS Genet

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Sperm deliver the male complement of DNA to the ovum, and thus play a key role in sexual reproduction. Accordingly, spermatogenesis has outstanding significance in fields as disparate as infertility treatments and pest-control, making it a broadly interesting and important focus for molecular genetics research in a wide range of species. Here we investigate spermatogenesis in the model lepidopteran insect Bombyx mori (silkworm moth), with particular focus on the gene PMFBP1 (polyamine modulated factor 1 binding protein 1). In humans and mouse, PMFBP1 is essential for spermatogenesis, and mutations of this gene are associated with acephalic spermatozoa, which cause infertility. We identified a B. mori gene labeled as “PMFBP1” in GenBank’s RefSeq database and sought to assess its role in spermatogenesis. Like in mammals, the silkworm version of this gene (BmPMFBP1) is specifically expressed in testes. We subsequently generated BmPMFBP1 mutants using a transgenic CRISPR/Cas9 system. Mutant males were sterile while the fertility of mutant females was comparable to wildtype females. In B. mori, spermatogenesis yields two types of sperm, the nucleated fertile eupyrene sperm, and anucleated unfertile apyrene sperm. Mutant males produced abnormal eupyrene sperm bundles but normal apyrene sperm bundles. For eupyrene sperm, nuclei were mislocated and disordered inside the bundles. We also found the BmPMFBP1 deficiency blocked the release of eupyrene sperm bundles from testes to ejaculatory seminalis. We found no obvious abnormalities in the production of apyrene sperm in mutant males, and double-matings with apyrene-deficient sex-lethal mutants rescued the ΔBmPMFBP1 infertility phenotype. These results indicate BmPMFBP1 functions only in eupyrene spermatogenesis, and highlight that distinct genes underlie the development of the two sperm morphs commonly found in Lepidoptera. Bioinformatic analyses suggest PMFBP1 may have evolved independently in lepidoptera and mammals, and that despite the shared name, are likely not homologous genes.

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Section: Resultsmentioning

confidence: 99%

BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

Yang

Chen

et al. 2022

PLoS Genet

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“…CNTLN facilitates equal segregation of cytoplasmic contents between daughter cells by recruiting the endosomal sorting complex required for transport machinery at the midbody ( 49 ). During sperm production, CNTLN, cooperating with SUN5 and PMFBP1, participates in HTCA assembly and integration of sperm head to the tail ( 50 ). In our results, the higher mRNA level in LCs indicated that cell proliferation was more obvious in them.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak

Wang

Afedo

et al. 2022

Front. Vet. Sci.

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In this study, we detected the expression of mRNAs, lncRNAs, and miRNAs in primary cultured leydig cells (LCs) and sertoli cells (SCs) of yak by RNA sequencing technology. A total of 84 differently expression mRNAs (DEmRNAs) (LCs vs. SCs: 15 up and 69 down), 172 differently expression lncRNAs (DElncRNAs) (LCs vs. SCs: 36 up and 136 down), and 90 differently expression miRNAs (DEmiRNAs) (LCs vs. SCs: 72 up and 18 down) were obtained between the two types of cells. GO enrichment and KEGG analysis indicated that the differential expression genes (DEGs) were more enriched in the regulation of actin cytoskeleton, Rap1/MAPK signaling pathway, steroid biosynthesis, focal adhesion, and pathways associated with metabolism. Targeted regulation relationship pairs of 3β-HSD and MSTRG.54630.1, CNTLN and MSTRG.19058.1, BRCA2 and MSTRG.28299.4, CA2 and novel-miR-148, and ceRNA network of LAMC3-MSTRG.68870.1- bta-miR-7862/novel-miR-151/novel-miR-148 were constructed by Cytoscape software. In conclusion, the differences between LCs and SCs were mainly reflected in steroid hormone synthesis, cell proliferation and metabolism, and blood-testicular barrier (BTB) dynamic regulation, and 3β-HSD, CNTLN, BRCA2, CA2, and LAMC3 may be the key factors causing these differences, which may be regulated by ncRNAs. This study provides a basic direction for exploring the differential regulation of LCs and SCs by ncRNAs.

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“…Failure to establish or maintain the connection between the head and tail can result in acephalic spermatozoa syndrome in patients, which presents with unstable connections between the sperm head and tail, including bent configurations and completely detached sperm heads, ultimately resulting in infertility (Baccetti et al, 1989, Chemes et al, 1987, Chemes et al, 1999, Perotti et al, 1981). Defects at the connection between the sperm head and tail have been linked to infertility across many other species, including bulls (Blom and Birch-Andersen, 1970), mice (Wang et al, 2022, Zhang et al, 2021), and Drosophila (Anderson et al, 2009, Kracklauer et al, 2010, Li et al, 2004, Sitaram et al, 2012, Texada et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

The Proximal Centriole-Like Structure Anchors the Centriole to the Sperm Nucleus

Buglak,

Holmes,

Galletta

et al. 2024

Preprint

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Proper connection between the sperm head and tail is critical for sperm motility and fertilization. The link between the head and tail is mediated by the Head-Tail Coupling Apparatus (HTCA), which secures the axoneme (tail) to the nucleus (head). However, the molecular architecture of the HTCA is not well understood. Here, we use Drosophila to create a high-resolution map of proteins and structures at the HTCA throughout spermiogenesis. Using structured illumination microscopy, we demonstrate that key HTCA proteins Spag4 and Yuri form a Centriole Cap that surrounds the centriole (or Basal Body) as it is inserted, or embedded into the surface of the nucleus. As development progresses, the centriole is laterally displaces to the side of the nucleus, during which time the HTCA expands under the nucleus, forming what we term the Nuclear Shelf. We next show that the proximal centriole-like (PCL) structure is positioned under the Nuclear Shelf and functions as a critical stabilizer of the centriole-nuclear attachment. Together, our data indicate that the HTCA is complex, multi-point attachment site that simultaneously engages the PCL, the centriole, and the nucleus to ensure proper head-tail connection during late-stage spermiogenesis.

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The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus

Cited by 34 publications

References 46 publications

BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori

Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak

The Proximal Centriole-Like Structure Anchors the Centriole to the Sperm Nucleus

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