The mitochondria-rich cell of frog skin as hormone-sensitive “shunt-path”

Voûte, Cornelis L.; Meier, Wolfgang

doi:10.1007/bf02026003

Cited by 81 publications

(44 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, our results overruled this hypothesis, since no difference in SR-B1 known to be rich in mitochondria and to show endo cytic activity ( 25 ). It was suggested that they are involved in fl uid acidifi cation because of their high carbonic anhydrase content ( 26 ) as well as in sodium transport and chloride fl uxes ( 27 ). Our data suggest that these cells are also critical for cholesterol traffi cking in the proximal epididymis.…”

Section: Ce Accumulation Enhances Apoptosis In Segment 1 Epithelial Cmentioning

confidence: 47%

LXR and ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell-specific manner

Ouvrier¹,

Cadet²,

Vernet³

et al. 2009

Journal of Lipid Research

View full text Add to dashboard Cite

This article is available online at http://www.jlr.org tozoa to fully mature cells ( 1 , 2 ). Principal aspects of epididymal transformation include functional maturation, sperm concentration, storage of the spermatozoa in a quiescent state, and removal of degenerating cells. Functional maturation of these spermatozoa requires interaction of sperm with luminal fl uid, whose composition is regulated by absorption and secretion activities of the epididymal epithelium ( 3 ).The sperm plasma membrane is the site of dramatic changes throughout the development of these cells, and, in the epididymis, the remodeling process includes important modifi cations in lipid composition as well as repositioning of lipid and protein components to different membrane domains ( 4 ). The lipid composition of mammalian spermatozoa is specifi c with a large amount of plasmalogen phospholipids (PLs), a high content of long-chain PUFAs, mostly arachidonic acid (20:4, n-6), docosapentaenoic acid (22:5, n-3), and docosahexaenoic acid (22:6, n-3), and relatively low cholesterol:PL ratios ranging from 0.24 for caput epididymidis sperm to 0.29 for cauda epididymidis sperm in mice ( 5 ). This ratio is an indicator of membrane fl uidity, and although it is quite stable during epididymal transit, membrane fl uidity was shown to increase during this step ( 6 ). Earlier work showed a significant decrease of the two major PLs, phosphatidylcholine and phosphatidylethanolamine, in mouse spermatozoa from cauda epididymidis compared with those from caput epididymidis ( 5 ). During epididymal maturation, stearic acid (C18:0) decreased also, whereas palmitic acid (C16:0) increased. Among the PUFAs, docosapentaenoic and docosahexaenoic acids rose signifi cantly in the cauda epididymidis. Cholesterol content also declined by roughly 65% during epididymal maturation ( 5 ). These modifi cations have been supposed to prepare sperm cells for capacitation and acrosome reaction that take place in the Abstract Mammalian spermatozoa undergo important plasma membrane maturation steps during epididymal transit. Among these, changes in lipids and cholesterol are of particular interest as they are necessary for fertilization. However, molecular mechanisms regulating these transformations inside the epididymis are still poorly understood. Liver X receptors (LXRs), the nuclear receptors for oxysterols, are of major importance in intracellular cholesterol homeostasis, and LXR ؊ / ؊ -defi cient male mice have already been shown to have reduced fertility at an age of 5 months and complete sterility for 9-month-old animals. This sterility phenotype is associated with testes and caput epididymides epithelial defects. The research presented here was aimed at investigating how LXRs act in the male caput epididymidis by analyzing key actors in cholesterol homeostasis. We show that accumulation of cholesteryl esters in LXR The epididymis is an essential organ for reproductive physiology as sperm cells start the process of posttesticular maturation during their transit in this or...

show abstract

Section: Ce Accumulation Enhances Apoptosis In Segment 1 Epithelial Cmentioning

confidence: 47%

LXR and ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell-specific manner

Ouvrier¹,

Cadet²,

Vernet³

et al. 2009

Journal of Lipid Research

View full text Add to dashboard Cite

show abstract

“…It has both a proton pump and a Cl -/HCO3 -exchanger in the apical membrane, together with the Na channel. It has, in addition, a high conductance, voltage and concentration-activated Cl -channel in the apical membrane (Voûte and Meier, 1978), although this channel would be closed in normal FW ([Cl -] <5·mmol·l -1 ). The γMR cell is shown in Fig.·2.…”

Section: The Model(s)mentioning

confidence: 99%

The mechanism of sodium chloride uptake in hyperregulating aquatic animals

Kirschner

2004

Journal of Experimental Biology

183

125

View full text Add to dashboard Cite

show abstract

“…The epithelium of the frog skin consists of four to six layers of principal cells in addition to flask-shaped mitochondria-rich cells (Vofite and Meier, 1978). The underlying dermis contains several types of glands with ducts opening into the apical surface (Vofite, 1963;Farquhar and Palade, 1964).…”

Section: Origin Of K and CL Channelsmentioning

confidence: 99%

“…The underlying dermis contains several types of glands with ducts opening into the apical surface (Vofite, 1963;Farquhar and Palade, 1964). In the collagenase split epithelium most of the glands are destroyed (Vofite and Meier, 1978;Thompson and Mills, 1981) but some isolated gland cells may remain attached to the epithelium. Mitochondria-rich cells comprise ~ 1-5% of the apical surface area and of the total cell volume of frog and toad skin epithelia (Vofite and Meier, 1978;Larsen, Ussing, and Spring, 1987).…”

Section: Origin Of K and CL Channelsmentioning

confidence: 99%

“…In the collagenase split epithelium most of the glands are destroyed (Vofite and Meier, 1978;Thompson and Mills, 1981) but some isolated gland cells may remain attached to the epithelium. Mitochondria-rich cells comprise ~ 1-5% of the apical surface area and of the total cell volume of frog and toad skin epithelia (Vofite and Meier, 1978;Larsen, Ussing, and Spring, 1987). Most cells in the preparation used in these studies had a granular appearance, although occasionally a small number of flask-shaped cells were present.…”

Section: Origin Of K and CL Channelsmentioning

confidence: 99%

See 1 more Smart Citation

Whole-cell and single channel K+ and Cl- currents in epithelial cells of frog skin.

1991

The Journal of General Physiology

View full text Add to dashboard Cite

A B S T R A C T Whole-cell and single channel currents were studied in cells from frog (R. pipiens and R. catesbiana) skin epithelium, isolated by collagenase and trypsin treatment, and kept in primary cultures up to three days. Whole-cell currents did not exhibit any significant time-dependent kinetics under any ionic conditions used. With an external K gluconate Ringer solution the currents showed slight inward rectification with a reversal potential near zero and an average conductance of 5 nS at reversal. Ionic substitution of the external medium showed that most of the cell conductance was due to K and that very little, if any, Na conductance was present. This confirmed that most cells originate from inner epithelial layers and contain membranes with basolateral properties. At voltages more positive than 20 mV outward currents were larger with K in the medium than with Na or N-methyl-Dglucamine. Such behavior is indicative of a multi-ion transport mechanism. Wholecell K current was inhibited by external Ba and quinidine. Blockade by Ba was strongly voltage dependent, while that by quinidine was not. In the presence of high external CI, a component of outward current that was inhibited by the anion channel blocker diphenylamine-2-carboxylate (DPC) appeared in 70% of the cells. This component was strongly outwardly rectifying and reversed at a potential expected for a C1 current. At the single channel level the event most frequently observed in the cell-attached configuration was a K channel with the following characteristics: inward-rectifying I-V relation with a conductance (with 112.

show abstract

The mitochondria-rich cell of frog skin as hormone-sensitive “shunt-path”

Cited by 81 publications

References 20 publications

LXR and ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell-specific manner

LXR and ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell-specific manner

The mechanism of sodium chloride uptake in hyperregulating aquatic animals

Whole-cell and single channel K+ and Cl- currents in epithelial cells of frog skin.

Contact Info

Product

Resources

About