The mitochondrial genome of Atrocalopteryx melli Ris, 1912 (Zygoptera: Calopterygidae) via Ion Torrent PGM NGS sequencing

Abstract

The mitochondrial genome of Atrocalopteryx melli was sequenced and assembled via Next-Generation Sequencing (NGS) and iteratively assembly process with a reference seed. This genome is 15,562 bp long and A þ T biased (71%), with 37 genes arranged in common order of Odonata. All protein-coding genes are initiated by typical "ATN" codon, and 9 genes are terminated with a complete stop codon, except nad4, nad5, cox2, and cox3, which are terminated with an incomplete codon "T(aa)". The S5 intergenic spacer is absent in this genome, supporting that lacking of S5 as a specific character for damselflies. The A þ T rich region of A. melli is 267 bp longer than that of A. atrata. This mitogenome provides new molecular information for understanding of A. melli and Atrocalopteryx.

The mitochondrial genome of Phyllodiaptomus tunguidus Shen & Tai, 1964 (Copepoda, Calanoida) and its phylogeny

“…Genomic DNA was sequenced using a next-generation method. The assembly and annotation procedure followed Xu et al (2018) with the COI sequence of P. tunguidus as seed. The mitochondrial genome was annotated with the MITOS WebServer (Bernt et al 2013) and verify via BLAST (Altschul et al 1990).…”

“…The assembly and annotation procedure followed Xu et al. ( 2018 ) with the COI sequence of P. tunguidus as seed. The mitochondrial genome was annotated with the MITOS WebServer (Bernt et al.…”

The mitochondrial genome of Diaphanosoma excisum Sars, 1885 (Crustacea: Branchiopoda: Cladocera) from Hainan Island, China

“…Thousands of individuals were collected and preserved in À80 C. Genomic DNA was extracted using the TIANamp Marine Animals DNA Kit (TIANGEN BIOTECH CO., LTD (Beijing, China) and sequenced using a next-generation method on Illumina platform HiSeq 2500. The assembly and annotation procedure followed Xu et al (2018) with the COI sequence of D. excisum as the seed. The assembled sequence was annotated in MITOS WebServer (Bernt et al 2013).…”

The mitochondrial genome of Diaphanosoma dubium with comparison with Daphnia magna

“…The assembly and annotation procedure followed Xu et al. ( 2017 ) with COI sequence of Diaphanosoma dubium (AB549201) as seed. COI of four other Diaphanosoma species ( D. excisum , D. orghidani , D. brachyurum and D. mongolianum ) were amplified with 2 pairs of primers: LCO1490 and HCO2198 (Vrijenhoek 1994 ), CrustF (Costa et al.…”

“…Thousands of individual was gathered after 1 moth of cultivation, and the collection was preserved in À80 C before sending to Beijing Genomics Institute (BGI) for next-generation sequencing (Hiseq 2500, PE-150). The assembly and annotation procedure followed Xu et al (2017) with COI sequence of Diaphanosoma dubium (AB549201) as seed. COI of four other Diaphanosoma species (D. excisum, D. orghidani, D. brachyurum and D. mongolianum) were amplified with 2 pairs of primers: LCO1490 and HCO2198 (Vrijenhoek 1994), CrustF (Costa et al 2007) and HCO2198.…”