The mitochondrial genome of the phytopathogenic basidiomycete Moniliophthora perniciosa is 109kb in size and contains a stable integrated plasmid

Formighieri, Eduardo Fernandes; Tiburcio, Ricardo Augusto; Armas, Eduardo D.; Medrano, Francisco J.; Shimo, Hugo; Carels, Nicolas; Góes-Neto, Aristóteles; Cotomacci, Carolina; Carazzolle, Marcelo Falsarella; Sardinha-Pinto, Naiara; Thomazella, Daniela P. T.; Rincones, Johana; Digiampietri, Luciano Antônio; Carraro, Dirce Maria; Azeredo-Espin, Ana Maria Lima de; Reis, Sérgio Furtado dos; Deckmann, Ana Carolina; Gramacho, Karina Peres; Gonçalves, Marilda Souza; Neto, José Pereira Moura; Barbosa, Luciana Veiga; Meinhardt, Lyndel W.; Cascardo, Júlio Cézar M.; Pereira, Gonçalo Amarante Guimarães

doi:10.1016/j.mycres.2008.04.014

Cited by 79 publications

(80 citation statements)

References 88 publications

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“…According to Formighieri et al (2008), the disruptive integration of a plasmid into the mitochondrial genome of M. perniciosa was a recent event, and may have been associated with the development of certain biotypes. This process may have occurred through the crossing of distantly related lineages by the mitotic or meiotic mechanisms previously mentioned, or by mycoviruses.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of DNA and RNA polymerases from a Moniliophthora perniciosa mitochondrial plasmid reveals probable lateral gene transfer

Andrade

Goés-Neto

2015

Genet. Mol. Res.

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ABSTRACT. The filamentous fungus Moniliophthora perniciosa is a hemibiotrophic basidiomycete that causes witches' broom disease of cacao (Theobroma cacao L.). Many fungal mitochondrial plasmids are DNA and RNA polymerase-encoding invertrons with terminal inverted repeats and 5'-linked proteins. The aim of this study was to carry out comparative and phylogenetic analyses of DNA and RNA polymerases for all known linear mitochondrial plasmids in fungi. We performed these analyses at both gene and protein levels and assessed differences between fungal and viral polymerases in order to test the lateral gene transfer (LGT) hypothesis. We analyzed all mitochondrial plasmids of the invertron type within the fungal clade, including five from Ascomycota, seven from Basidiomycota, and one from Chytridiomycota. All phylogenetic analyses generated similar tree topologies regardless of the methods and datasets used. It is likely that DNA and RNA polymerase genes were inserted into the mitochondrial genomes 14105-14114 (2015) of the 13 fungal species examined in our study as a result of different LGT events. These findings are important for a better understanding of the evolutionary relationships between fungal mitochondrial plasmids.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of DNA and RNA polymerases from a Moniliophthora perniciosa mitochondrial plasmid reveals probable lateral gene transfer

Andrade

Goés-Neto

2015

Genet. Mol. Res.

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“…A typical fungal mt genome will contain 30-40 genes (Formighieri et al 2008). This is usually made up of a set of 14 standard genes (ATP synthase, subunits 6, 8, and 9; cytochrome c oxidase, subunits 1, 2, and 3; NADH dehydrogenase, subunits 1, 2, 3, 4, 4L, 5, and 6; apocytochrome b) (Arora et al 2004); the mitochondrial large and small ribosomal subunits; and a set of tRNAs .…”

Section: Introductionmentioning

confidence: 99%

Sequence and structure of the linear mitochondrial genome of Pneumocystis carinii

et al. 2009

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With the exception of a few genes, most of the mitochondrial (mt) genome of Pneumocystis carinii has not previously been sequenced. Shotgun sequences generated as a result of the Pneumocystis Genome Project (PGP) were assembled with the gap4 assembly program into a 23-kb contig. Annotation of the mt genome identified 4 open reading frames and 20 tRNAs in addition to 17 other genes: ATP synthase, subunits 6, 8, and 9; cytochrome c oxidase, subunits 1, 2, and 3; NADH dehydrogenase, subunits 1, 2, 3, 4, 4L, 5, and 6; apocytochrome b; RNase P RNA gene; and the mitochondrial large and small ribosomal RNA subunits. A 24-bp unit that repeated from one to five times was identified interior to the ends of the mt genome. Migration of the genome on CHEF gels was consistent with that of linear DNA and digestion with BAL31 showed a concomitant reduction in size of the genome, a characteristic of linear DNA. Together with the identification of terminal repeats similar to those found in other linear fungal mt genomes and the inability to join the ends by PCR, these data provide strong evidence that the mt genome of P. carinii is linear.

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“…Although no prior information about the probable function of these enzymes in the metabolism of M. perniciosa is available (Formighieri et al, 2008), these molecules are implicated in the replication mechanism and expression of the mitochondrial genome (Kempken, 1994;Kennell and Cohen, 2005) through a degree of structural and sequence identity with other fungal plasmid polymerases (Andrade et al, 2009). Given this function, the activity of these enzymes may contribute to increasing the survival of the fungus, as described in the pAL2 plasmid of Podospora anserina (Hermanns et al, 1994;Maas et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…This plasmid displays the characteristics of previously described linear mitochondrial plasmids (Kennell and Cohen, 2008) such as the presence of large terminal inverted repeats and 2 large open reading frames on opposite strands (Kempken, 1994). Moreover, the M. perniciosa linear-type plasmid encodes virallike DNA and RNA polymerases (Pöggeler and Kempken, 2004;Formighieri et al, 2008). Linear plasmids are commonly related to fungal senescence and longevity (Pöggeler and Kempken, 2004) owing to mitochondrial instability and plasmid insertions into the mitochondrial genome (Bertrand et al, 1985(Bertrand et al, , 1986Bertrand, 2000).…”

Section: Introductionmentioning

confidence: 99%

DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress

Andrade¹,

Dias²,

Gomes³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Moniliophthora perniciosa (Stahel) Aime and PhillipsMora is a hemibiotrophic basidiomycete (Agaricales, Tricholomataceae) that causes witches' broom disease in cocoa (Theobroma cacao L.). This pathogen carries a stable integrated invertron-type linear plasmid in its mitochondrial genome that encodes viral-like DNA and RNA polymerases related to fungal senescence and longevity. After culturing the fungus and obtaining its various stages of development in triplicate, we carried out total RNA extraction and subsequent complementary DNA synthesis. To analyze DNA and RNA polymerase expression levels, we performed real-time reverse transcriptase polymerase chain reaction for various fungal phases of development. Our results showed that DNA and RNA polymerase gene 1945 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (2): 1944-1950 (2013 M. perniciosa mitochondrial plasmid and oxidative stress expression in the primordium phase of M. perniciosa is related to a potential defense mechanism against T. cacao oxidative attack.

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The mitochondrial genome of the phytopathogenic basidiomycete Moniliophthora perniciosa is 109kb in size and contains a stable integrated plasmid

Cited by 79 publications

References 88 publications

Phylogenetic analysis of DNA and RNA polymerases from a Moniliophthora perniciosa mitochondrial plasmid reveals probable lateral gene transfer

Phylogenetic analysis of DNA and RNA polymerases from a Moniliophthora perniciosa mitochondrial plasmid reveals probable lateral gene transfer

Sequence and structure of the linear mitochondrial genome of Pneumocystis carinii

DNA and RNA polymerase activity in a Moniliophthora perniciosa mitochondrial plasmid and self-defense against oxidative stress

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