The Mitogen-Activated Protein Kinase Dependent Expression of Prostaglandin H Synthase-2 and Interleukin-8 Messenger Ribonucleic Acid by Myometrial Cells: The Differential Effect of Stretch and Interleukin-1β

Sooranna, Suren R.; Loudon, J. A. Z.; Terzidou, Vasso; Bennett, Phillip R.; Johnson, Mark R.

doi:10.1210/jc.2004-1390

Cited by 78 publications

(59 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, mechanical stretch has been shown to increase IL-8 secretion from alveolar epithelial cells (21,22) and myometrial cells (12,18). Mechanical stretch also upregulates the expression of cyclooxygenase-2 in ESC (our unpublished data) as well as in myometrial cells (19).…”

Section: Discussionsupporting

confidence: 55%

Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells

Harada¹,

Osuga²,

Takemura³

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

Recently, uterine movement-induced mechanical stress was noticed to have possible effects on endometrial functions. In this study, we addressed the possible effect of mechanical stress on the process of decidualization of endometrial stromal cells (ESC). ESC were cultured on flexiblebottomed culture plates. After decidualization was achieved with estradiol and progesterone for 12 days, cultures were continued for 24 h with or without cyclic stretch (25% elongation) in serum-free conditions at a rate of 2 cycles/min using a computer-operated cell tension system. Concentrations of insulin-like growth factor-binding protein-1 (IGFBP-1), a marker of decidualization, in the conditioned medium were measured by specific ELISA, and IGFBP-1 mRNA expression in the ESC was measured by RT-PCR. Cyclic stretch remarkably increased IGFBP-1 secretion from decidualized ESC. It also increased IGFBP-1 mRNA in decidualized ESC. The increase in IGFBP-1 secretion was inhibited by actinomycin D but not by indomethacin, PD-98059, or H-89. Conditioned medium of decidualized ESC cultured with cyclic stretch increased IGFBP-1 secretion from decidualized ESC cultured under stationary conditions. These findings imply that uterine movement modulates decidualization of the endometrium and has a regulatory effect on reproduction.insulin-like growth factor-binding protein-1; endometrium; decidualization; prostaglandin E2; indometacin DECIDUALIZATION, a process of endometrial differentiation, is essential for embryo implantation and maintenance of pregnancy. During the process of decidualization, characteristics of endometrial cells change profoundly. This was demonstrated by marked alterations in the expression of numerous genes in decidualized human endometrial stromal cells (ESC) in vitro (17). Progesterone is a well-known inducer of decidualization, whereas prostaglandin E 2 (PGE 2 ) and relaxin enhance the process via activation of cAMP signaling (8,11,16,20). However, the complex mechanisms that regulates decidualization remains to be elucidated.Recently, uterine movement, a mechanical process, has been noticed to play important roles in fertility (4). Subendometrial myometrium exerts wave-like activity throughout the menstrual cycle (3, 13), and the movement is believed to support embryo and sperm transfer. The peristaltic uterine movement is also suggested to be involved in other uterus-related events such as endometriosis and menstruation. Recently, we (9) suggested that the subendometrial myometrial movement is transduced to a biochemical signal in the endometrium and may play roles in endometrium-related physiological and pathological events.Given that the mechanical stress associated with uterine peristalsis alters biochemical characteristics of the endometrium, it is plausible that decidualization, a differentiation process of the endometrium, may be affected by the uterine movement. To address the issue, we conducted an in vitro study to see the effect of mechanical stretch on the production of IGFBP-1, a representative marker of d...

show abstract

Section: Discussionsupporting

confidence: 55%

Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells

Harada¹,

Osuga²,

Takemura³

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…For instance, Sales et al (2009) demonstrated that PGF2A upregulation of PTGS2 expression is dependent on PLC and ERK1/2 but not PKC signalling pathways in endometrial adenocarcinoma cells. Some studies reported that ERK and P38 MAPK activation induces PTGS2 expression in HMSMCs (Bartlett et al 1999, Sooranna et al 2005, and calcineurin/NFAT signalling pathway is involved in PTGS2 expression in human myomentrium (Pont et al 2012) and endometrial stromal cells (Abraham et al 2012). For OTR, Sooranna et al (2007) have demonstrated that activation of P38 signalling leads to an increase in OTR expression in human myometrial cells.…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin F2α regulates the expression of uterine activation proteins via multiple signalling pathways

Xu¹,

You²,

Liu³

et al. 2015

Reproduction

View full text Add to dashboard Cite

Prostaglandin F2a (PGF2A) has multiple roles in the birth process in addition to its vital contractile role. Our previous study has demonstrated that PGF2A can modulate uterine activation proteins (UAPs) in cultured pregnant human myometrial smooth muscle cells (HMSMCs). The objective of this study was to define the signalling pathways responsible for PGF2A modulation of UAPs in myometrium. It was found that PGF2A stimulated the expression of (GJA1) connexin 43 (CX43), prostaglandin endoperoxide synthase 2 (PTGS2) and oxytocin receptor (OTR) in cultured HMSMCs. The inhibitors of phospholipase C (PLC) and protein kinase C (PKC) blocked PGF2A-stimulated expression of CX43. The inhibitors of ERK, P38 and NFkB also blocked the effect of PGF2A on CX43 expression, whereas PI3K and calcineurin/nuclear factor of activated T-cells (NFAT) pathway inhibitors did not reverse the effect of PGF2A on CX43. For PTGS2 and OTR, PLC, PI3K, P38 and calcineurin/NFAT signalling pathways were involved in PGF2A action, whereas PKC and NFkB signalling were not involved. In addition, PGF2A activated NFAT, PI3K, NFkB, ERK and P38 signalling pathways. Our data suggest that PGF2A stimulates CX43, PTGS2 and OTR through divergent signalling pathways.

show abstract

“…These pro-inflammatory actions of AGE-BSA were elicited through a number of intracellular signalling pathways, namely ERK 1/2 and NF-kB. AGE can induce the expression and activation of a number of transcription factors including NF-kB and MAP kinase (Smith et al 1994, Nishino et al 1995, Anderson et al 1999, both of which have been implicated in the processes of human labour and delivery (Lappas et al 2003, Jung et al 2005, Sooranna et al 2005. Similarly in this study, we have demonstrated that AGE-BSA induces an increase in NF-kB p65 DNA binding activity and ERK 1/2 phosphorylation in human gestational tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Ligand-receptor interaction, through the generation of reactive oxygen species (ROS), induces sustained post-receptor signalling including activation of p21ras, the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK)-1 and -2, p38 MAP kinase, and downstream consequences such as activation of nuclear factor kB (NF-kB), which subsequently induces the expression of adhesion molecules, cytokines and chemokines (reviewed in Bucciarelli et al 2002, Chavakis et al 2004, Bierhaus et al 2005, Ramasamy et al 2005. We, and other researchers, have previously demonstrated that in human gestational tissues, ERK 1/2 and NF-kB regulate proinflammatory proteins including cytokines, prostaglandins and proteases (Lappas et al 2003, Jung et al 2005, Sooranna et al 2005, all of which are known to be increased in human labour.…”

Section: Introductionmentioning

confidence: 99%

Advanced glycation endproducts mediate pro-inflammatory actions in human gestational tissues via nuclear factor-κB and extracellular signal-regulated kinase 1/2

Lappas¹,

Permezel²,

Rice³

2007

Journal of Endocrinology

View full text Add to dashboard Cite

Processes of human labour include increased oxidative stress, formation of inflammatory mediators (e.g. cytokines) and uterotonic phospholipid metabolites (e.g. prostaglandins). In non-gestational tissues, advanced glycation endproducts (AGE) induce the expression of pro-inflammatory molecules through mitogen-activated protein kinase and nuclear factor kB (NF-kB)-dependent pathways. Thus, the aim of this study was to investigate the effects of AGE on 8-isoprostane (a marker of oxidative stress), pro-inflammatory cytokine and prostaglandin release in human gestational tissues, and to define the signalling pathways involved. Human placenta and gestational membranes (amnion and choriodecidua combined; nZ5) were incubated in the absence or presence of AGE-BSA (0 . 25, 0 . 5, 1 and 2 mg/ml) for 18 h. AGE significantly increased in vitro release of tumour necrosis factor-a, interleukin (IL)-1b, IL-6, IL-8, prostaglandin (PG)E 2 , PGF 2a and 8-isoprostane from human placenta and gestational membranes. This was associated with a concomitant increase in NF-kB p65 activation and ERK 1/2 phosphorylation. AGE-stimulated 8-isoprostane, cytokine and prostaglandin production was significantly suppressed by the ERK 1/2 inhibitor U0126 and the NF-kB inhibitor BAY 11-7082. In conclusion, AGE mediates inflammatory actions in human gestational tissues. Protein kinases and the NF-kB pathway play an essential role in AGE signalling in human gestational tissues.

show abstract

The Mitogen-Activated Protein Kinase Dependent Expression of Prostaglandin H Synthase-2 and Interleukin-8 Messenger Ribonucleic Acid by Myometrial Cells: The Differential Effect of Stretch and Interleukin-1β

Cited by 78 publications

References 56 publications

Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells

Mechanical stretch upregulates IGFBP-1 secretion from decidualized endometrial stromal cells

Prostaglandin F2α regulates the expression of uterine activation proteins via multiple signalling pathways

Advanced glycation endproducts mediate pro-inflammatory actions in human gestational tissues via nuclear factor-κB and extracellular signal-regulated kinase 1/2

Contact Info

Product

Resources

About