The mixture of cashew nut shell liquid and castor oil results in an efficient larvicide against Aedes aegypti that does not alter embryo-fetal development, reproductive performance or DNA integrity

Abstract

Dengue fever, chikungunya fever and Zika virus are epidemics in Brazil that are transmitted by mosquitoes, such as Aedes aegypti or Aedes albopictus. The liquid from shells of cashew nuts is attractive for its important biological and therapeutic activities, which include toxicity to mosquitoes of the genus Aedes. The present study evaluated the effects of a mixture of surfactants from natural cashew nutshell liquid and castor oil (named TaLCC-20) on the mortality of larvae and on the reproductive performance, embryonic and fetal development and genetic stability of Swiss mice. A total of 400 Ae. aegypti larvae (third larval stage) were treated with TaLCC-20 concentrations of 0.05 mg/L, 0.5 mg/L, or 5 mg/L (ppm). Twenty pregnant female mice were also orally administered TaLCC-20 at doses of 5 mg/kg and 50 mg/kg body weight (b.w.), and 10 animals were given only drinking water at 0.1 mL/10 g b.w. (orally). The results of a larvicide test demonstrated that 5 mg/mL TaLCC-20 killed 100% of larvae within three hours, which is comparable to the gold standard indicated by the Ministry of Health. Overall, these results show that TaLCC-20 is an efficient larvicide that does not induce genetic damage. In addition, changes in reproductive performance and embryo-fetal development appear positive, and the formulation is cost effective. Therefore, TaLCC-20 is an important product in the exploration of natural larvicides and can assist in fighting mosquitos as vectors for dengue fever, chikungunya fever and Zika virus, which are emerging/re-emerging and require proper management to ensure minimal harm to the human population. Therefore, TaLCC-20 can be considered a key alternative to commercial products, which are effective yet toxigenic.

Curcumin/d‐mannitol as photolarvicide: induced delay in larval development time, changes in sex ratio and reduced longevity of Aedes aegypti

“…Photobleaching was assessed accordingly to Supporting Information, Section S1.3. Residual activity assays were conducted according to adapted methods of Vani et al, 31 using two different concentrations: LC 99‐24h (0.4 mg L −1 of curcumin), named DMC 1×, and 10× the LC 99‐24h (4 mg L −1 ), named DMC 10×. Groups of 15 larvae (L3) were placed into transparent plastic containers with 200 mL of DMC solution in the respective concentrations and exposed to light.…”

Molecular characterization of the viral structural protein genes in the first outbreak of dengue virus type 2 in Hunan Province, inland China in 2018

“…Dengue fever (DF), an ancient disease with a history of approximately 2000 years, is caused by four different but closely related dengue viruses (DENV-1, DENV-2, DENV-3, and DENV-4) and is mainly transmitted by female Aedes aegypti or female Aedes albopictus [ 1 , 2 ]. DF occurs in tropical and subtropical urban and semi-urban areas around the world.…”

Molecular characterization of the viral structural protein genes in the first outbreak of dengue virus type 2 in Hunan Province, inland China in 2018

“…Dengue fever (DF) is an ancient disease with a history of approximately 200 years, which is caused by four different but closely related dengue viruses (DENV-1, DENV-2, DENV-3, and DENV-4) and is mainly transmitted by female Aedes aegypti or female Aedes albopictus [1,2]. It occurs in tropical and subtropical urban and semi-urban areas around the world.…”