1999
DOI: 10.1128/jb.181.9.2973-2978.1999
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The MobA-Linked Primase Is the Only Replication Protein of R1162 Required for Conjugal Mobilization

Abstract: Cells newly transformed with plasmid R1162 DNA were used as donors in conjugal matings to determine if the plasmid replication genes are necessary for transfer. An intact system for vegetative replication is not required for transfer at normal frequency, but the plasmid primase, in the form linked to the nickase, must be present in donor cells.

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Cited by 22 publications
(19 citation statements)
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“…1a). The primase is not strictly required for mobilization, but contributes to complementary strand synthesis in both donor and recipient cells (Henderson & Meyer, 1999;Becker & Meyer, 2002;Parker & Meyer, 2002).…”
Section: Mob Q Relaxosomes Mob Q Relaxasesmentioning
confidence: 99%
“…1a). The primase is not strictly required for mobilization, but contributes to complementary strand synthesis in both donor and recipient cells (Henderson & Meyer, 1999;Becker & Meyer, 2002;Parker & Meyer, 2002).…”
Section: Mob Q Relaxosomes Mob Q Relaxasesmentioning
confidence: 99%
“…4). We assumed that the mutation would not affect conjugative replication, where strand separation is brought about by transfer and does not require the plasmid helicase (Henderson and Meyer, 1999). A plasmid heteroduplex was constructed as before, with the mutation on the strand first transferred, and the normal base on the complementary strand (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The plasmid replication proteins required for mobilization were identified by constructing a set of strains containing the self‐transmissible mobilizing vector R751 (Willetts and Crowther, 1981), all the proteins required for plasmid mobilization, and different combinations of the plasmid replication proteins (Henderson and Meyer, 1999). Cells of these strains were then transformed by electroporation with a plasmid that does not encode any replication proteins, but does contain both the origin of replication ( oriV ) and the origin of transfer ( oriT ) of R1162.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Las tres proteínas son requeridas para la movilización: MobA corta y liga la hebra transferida (Bhattacharje et al, 1992) a través de una actividad localizada en su extremo N terminal. En su extremo C terminal tiene actividad primasa (similar a la actividad RepB') que está relacionada con el amplio rango de huéspedes de los plásmidos IncQ (Henderson y Meyer, 1999). La proteína MobC por su parte interviene en la localización y separación de las hebras en el oriT (Zhang y Meyer, 1997) y la proteína MobB estabiliza el complejo de las proteínas Mob en el oriT y tiene también funciones adicionales en la transferencia que han sido poco caracterizadas (Perwez y Meyer, 1999).…”
Section: Funciones De Transferencia En Los Plásmidos Tiunclassified