The Model of Acute Pancreatitis of Different Degree in Experiment

Abstract

Relevance of the topic. Acute pancreatitis (OP) is originally an aseptic inflammation of the pancreas, with possible damage to the surrounding tissues and individual organs, as well as systems, which is the third most common after acute appendicitis and acute cholecystitis in the number of patients. In this regard, an experimental model is needed to further determine the tactics of management and treatment of patients with acute pancreatitis of varying severity.
 Objective: to develop a universal model of acute pancreatitis with the ability to control the area of ​​pancreatic necrosis through the use of various overlays (distal or proximal to the confluence of the accessory pancreatic duct into the common bile duct) of the ligature, which will ensure the development of total or subtotal pancreatonecrosis.
 Materials and methods. Matured white Wistar rats weighing 250-300 g were chosen as objects for the study. The simulation of acute pancreatitis in the experiment was carried out using a 50% bile solution (pH = 6.0) followed by intraductal injection without cannulation of the major duodenal papilla. To control the degree of severity and area of ​​the pancreas lesion, we use the staged overlay of turnstiles at the confluence of the additional pancreatic ducts and the common bile duct, which ensures the development of acute pancreatitis of varying severity. On the 5th day, the rats were removed from the experiment, an autopsy was performed with the removal of pancreatic preparations for further histological examination. The evaluation of the immune and oxidative parameters of the blood of experimental animals. Statistical processing of the obtained results was performed using descriptive and variation statistics techniques.
 Results. In the course of this work, moderate and severe acute pancreatitis is modeled, which allows us to focus on the course of each subject to variation statistics, determining their reliability, which is an important condition for creating the necessary platform for further study of the management and treatment of this disease.
 Findings. I use the resulting model, we managed:
 - to obtain a high reliable result of operations with minimal death of experimental animals, i.e. high repeatability of positive results;
 - perform an accessible operation with obvious technical simplicity;
 - see the possibility of a simulated disease for a long time up to 5 or more days, which subsequently allows medical intervention and testing of new dosage forms and drugs for treatment and studying their effects.
 Thus, it is possible to judge the universality of this model of acute pancreatitis under experimental conditions.

Experimental assessment of immunoreactivity indices and effectiveness of pharmacotherapy schemes in surgical models of acute pancreatitis of various severity

“…Fifteen minutes later, ligation of the two pancreatic ducts, removal of the tourniquets, and layered wound closure were performed ( Fig. 1) (Nazarenko et al 2019).…”

“…This is why the interest to AP modeling in the experimental animals seems to be justified as it makes it possible to compare the clinical picture of the disease as well as developing laboratory and morphological changes in the internal organs. In addition, experimental models allow us to give an objective assessment of the effect of drugs and their combinations in the correction of identified laboratory disorders, including immunoreactivity disorders, which will not only extrapolate the data obtained to the clinic, but also provide the best results in the treatment of moderate and severe AP (Denysiuk et al 2015;Janisch and Gardner 2016;Schmidt et al 2017;Nazarenko et al 2019).…”