The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS

Eceiza, Mikel Vicente; Gil‐Monreal, Miriam; Barco-Antoñanzas, María; Zabalza, Ana; Royuela, Mercedes

doi:10.1016/j.jplph.2022.153720

Cited by 13 publications

(12 citation statements)

References 65 publications

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“…palmeri populations assured lower lipid peroxidation (an oxidative stress burst) and was related to plant glyphosate resistance 37,41 . Similarly, we observed greater APX and CAT activities in GR leaves exposed to glyphosate in relation to susceptible plants.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Enzyme and Cytochrome P450 activities are involved in horseweed (<em>Conyza sumatrensis</em>) Resistance to Glyphosate

Kubis¹,

Marques²,

Kitamura³

et al. 2022

Preprint

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The intensive global use of glyphosate has led to the evolution of glyphosate resistant (GR) weed species, including the economically damaging horseweed (Conyza sumatrensis). We evaluated the glyphosate resistance mechanisms of C. sumatrensis. While 5-enolpyruvylshikimate-3-phosphate synthase activity was similar between the glyphosate resistant (GR) and nonresistant biotypes, plants from the GR population accumulated lower shikimate levels than susceptible ones, suggesting the absence of target-site resistance mechanisms. Decreases over time in glyphosate concentrations in GR leaves were not accompanied by increases in glyphosate concentrations in their stem and roots, indicating lower glyphosate distribution rates in GR plants. The early appearance of aminomethylphosphonic acid (the main glyphosate metabolite) in leaves, as well as its presence only in the stems and roots of GR plants, suggests faster glyphosate metabolism in GR plants than in susceptible ones. GR plants treated with glyphosate also showed greater antioxidant (ascorbate peroxidase [APX] and catalase [CAT]) and cytochrome P450-enzyme activities, indicating their great capacity to avoid glyphosate-induced oxidative stress. Three non-target mechanisms (reduced glyphosate translocation, increased metabolism, and increased antioxidant activity) therefore confer glyphosate resistance in C. sumatrensis plants. This is the first time that APX, CAT and P450 enzyme activities are related to GR in C. sumatrensis.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Enzyme and Cytochrome P450 activities are involved in horseweed (<em>Conyza sumatrensis</em>) Resistance to Glyphosate

Kubis¹,

Marques²,

Kitamura³

et al. 2022

Preprint

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“…Lipid peroxidation was determined by spectrophotometric detection of malondialdehyde (Hodges et al, 1999). Samples (0.05 g) were homogenised and MDA-TBA complex was extracted as described in Eceiza et al (2022). Malondialdehyde-TBA complex content was measured at 532 nm and correction for unspecific turbidity and sugar-TBA complexes was done by subtracting the absorbance of the same sample at 600 and 400 nm respectively (Hodges et al, 1999;Verma and Dubey, 2003).…”

Section: Lipid Peroxidationmentioning

confidence: 99%

“…Glutathione was extracted from samples (0.1 g) in 1 mL 1 M HCl, derivatised with 5-iodoacetamide fluorescein (Eceiza et al, 2022) and reduced with tributylphosphine (Zinellu et al, 2005). Glutathione content was measured by capillary electrophoresis (CE) equipped with a laser-induced fluorescence detector (Zulet et al, 2015).…”

Section: Glutathione and Related Compoundsmentioning

confidence: 99%

“…Oxidised glutathione (GSSG) was reduced with 10% tributylphosphine and then total GSH, cysteine (Cys) and g-glutamyl-cysteine (GGC) were analysed by CE. GSSG was determined as the difference between both total and reduced GSH values (Eceiza et al, 2022).…”

Section: Glutathione and Related Compoundsmentioning

confidence: 99%

“…Under adequate conditions, ROS production and antioxidant activity are balanced, but stressful conditions usually lead to oxidative stress. As happens with other herbicides that inhibit amino acid biosynthesis, such as glyphosate (Ahsan et al, 2008;Gomes and Juneau, 2016;Ostera et al, 2016;de Freitas-Silva et al, 2017;Eceiza et al, 2022) or glufosinate (Takano et al, 2019), ALS inhibitors are thought to cause moderate oxidative stress secondarily, not being the main cause of plant death (Zabalza et al, 2007;Caverzan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Role of oxidative stress in the physiology of sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting acetolactate synthase

et al. 2023

Self Cite

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The aim of the present study was to elucidate the role of oxidative stress in the mode of action of acetolactate synthase (ALS) inhibiting herbicides. Two populations of Amaranthus palmeri S. Watson from Spain (sensitive and resistant to nicosulfuron, due to mutated ALS) were grown hydroponically and treated with different rates of the ALS inhibitor nicosulfuron (one time and three times the field recommended rate). Seven days later, various oxidative stress markers were measured in the leaves: H2O2, MDA, ascorbate and glutathione contents, antioxidant enzyme activities and gene expression levels. Under control conditions, most of the analysed parameters were very similar between sensitive and resistant plants, meaning that resistance is not accompanied by a different basal oxidative metabolism. Nicosulfuron-treated sensitive plants died after a few weeks, while the resistant ones survived, independently of the rate. Seven days after herbicide application, the sensitive plants that had received the highest nicosulfuron rate showed an increase in H2O2 content, lipid peroxidation and antioxidant enzymatic activities, while resistant plants did not show these responses, meaning that oxidative stress is linked to ALS inhibition. A supralethal nicosulfuron rate was needed to induce a significant oxidative stress response in the sensitive population, providing evidence that the lethality elicited by ALS inhibitors is not entirely dependent on oxidative stress.

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Glyphosate-based herbicide: Impacts, detection, and removal strategies in environmental samples

Ogunbiyi,

Akamo,

Oluwasanmi

et al. 2023

Groundwater for Sustainable Development

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The moderate oxidative stress induced by glyphosate is not detected in Amaranthus palmeri plants overexpressing EPSPS

Cited by 13 publications

References 65 publications

Antioxidant Enzyme and Cytochrome P450 activities are involved in horseweed (<em>Conyza sumatrensis</em>) Resistance to Glyphosate

Antioxidant Enzyme and Cytochrome P450 activities are involved in horseweed (<em>Conyza sumatrensis</em>) Resistance to Glyphosate

Role of oxidative stress in the physiology of sensitive and resistant Amaranthus palmeri populations treated with herbicides inhibiting acetolactate synthase

Glyphosate-based herbicide: Impacts, detection, and removal strategies in environmental samples

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