Self-Determination Theory (SDT) has been studied to comprehend human motivation, particularly in education. Numerous studies have been conducted at universities regarding online learning as a technology to mitigate the effects of COVID-19. On the basis of these expansions, however, there is a knowledge gap regarding what constitutes advancement, future direction, and research gaps regarding SDT in university online learning. This new systematic literature review analyzed 49 articles using PRISMA to bridge the knowledge gap. Currently, SDT research in online learning at university does not extensively integrate other theories and models, but there is a trend toward acceptance models and cognitive theories. Future research should incorporate additional SDT factors such as intrinsic motivation, external regulation, identified regulation, and amotivation in addition to autonomy, competence, and relatedness. As most research samples students, a research gap involving lecturers and mixed groups is suggested. The future is anticipated to be dominated by quantitative research, leaving qualitative and mixed methods as points of exploration. This review sheds light on the advancements, future direction, and research gaps regarding SDT in university-level online learning. It could serve as a basis for future research in SDT within the context of online education.