The Modulatory Effects of Mesenchymal Stem Cells on Osteoclastogenesis

Sharaf-Eldin, Wessam E.; Abu-Shahba, Nourhan; Mahmoud, Mohamed; El‐Badri, Nagwa

doi:10.1155/2016/1908365

Cited by 42 publications

(42 citation statements)

References 158 publications

(200 reference statements)

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“…We also found that the presence of macrophages reduced the secretion of OPG, the decoy RANKL receptor. OPG is known to be secreted by both macrophages and MSCs, so it is surprising that OPG protein in the supernatant was most dramatically reduced in the polarized macrophage (M1 and M2) co‐cultures with greater than 60% reduction compared to MSCs grown alone and a statistically significant reduction compared to undifferentiated M0‐MSCs . These findings suggest that the inflammatory environment mediated by macrophages negatively regulate OPG secretion in our co‐culture system and thus may affect osteoclast activity along the OPG‐RANKL axis.…”

Section: Discussionmentioning

confidence: 85%

Pro‐inflammatory M1 macrophages promote Osteogenesis by mesenchymal stem cells via the COX‐2‐prostaglandin E2 pathway

Loi

Nathan

et al. 2017

Journal Orthopaedic Research

168

121

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Bone fractures are among the most common orthopaedic problems that affect individuals of all ages. Immediately after injury, activated macrophages dynamically contribute to and regulate an acute inflammatory response that involves other cells at the injury site, including mesenchymal stem cells (MSCs). These macrophages and MSCs work in concert to modulate bone healing. In this study, we co-cultured undifferentiated M0, pro-inflammatory M1, and anti-inflammatory M2 macrophages with primary murine MSCs in vitro to determine the cross-talk between polarized macrophages and MSCs and their effects on osteogenesis. After 4 weeks of co-culture, MSCs grown with macrophages, especially M1 macrophages, had enhanced bone mineralization compared to MSCs grown alone. The level of bone formation after 4 weeks of culture was closely associated with prostaglandin E2 (PGE2) secretion early in osteogenesis. Treatment with celecoxib, a cyclooxygenase-2 (COX-2) selective inhibitor, significantly reduced bone mineralization in all co-cultures but most dramatically in the M1-MSC co-culture. We also found that the presence of macrophages reduced the secretion of osteoprotegerin (OPG), the decoy RANKL receptor, suggesting that macrophages may indirectly modulate osteoclast activity in addition to enhancing bone formation. Taken together, these findings suggest that an initial pro-inflammatory phase modulated by M1 macrophages promotes osteogenesis in MSCs via the COX-2-PGE2 pathway.

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Section: Discussionmentioning

confidence: 85%

Pro‐inflammatory M1 macrophages promote Osteogenesis by mesenchymal stem cells via the COX‐2‐prostaglandin E2 pathway

Loi

Nathan

et al. 2017

Journal Orthopaedic Research

168

121

View full text Add to dashboard Cite

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“…Quantitative reverse transcriptase‐polymerase chain reaction revealed an increased expression of the RANKL, also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11) with an FC (Log2) of 4.36 and FC 4.87 at days 21 and 40, respectively. During the process of the physiological bone remodeling RANKL, the main osteoclastic effector, is expressed in a membrane‐bound form on many mesenchymal cells including MSCs, osteoblasts, osteocytes, and chondrocytes . To maintain normal bone homeostasis, RANKL signaling must be properly regulated .…”

Section: Discussionmentioning

confidence: 99%

“…43 To maintain normal bone homeostasis, RANKL signaling must be properly regulated. 43 Downregulated genes were those that encode for (i) cell-to-cell adhesion, such as VCAM1 and COMP, In hASCs grown on biomaterial CLEC3B protein was found to be expressed up to day 40. CLEC3B protein, which binds Ca 2+ , was investigated because of its potential involvement in the bone mineral metabolism.…”

Section: Discussionmentioning

confidence: 99%

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

Mazzoni

D’Agostino

Iaquinta

et al. 2019

Stem Cells Translational Medicine

100

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Tissue engineering‐based bone graft is an emerging viable treatment modality to repair and regenerate tissues damaged as a result of diseases or injuries. The structure and composition of scaffolds should modulate the classical osteogenic pathways in human stem cells. The osteoinductivity properties of the hydroxylapatite‐collagen hybrid scaffold named Coll/Pro Osteon 200 were investigated in an in vitro model of human adipose mesenchymal stem cells (hASCs), whereas the clinical evaluation was carried out in maxillofacial patients. Differentially expressed genes (DEGs) induced by the scaffold were analyzed using the Osteogenesis RT2 PCR Array. The osteoinductivity potential of the scaffold was also investigated by studying the alkaline phosphatase (ALP) activity, matrix mineralization, osteocalcin (OCN), and CLEC3B expression protein. Fifty patients who underwent zygomatic augmentation and bimaxillary osteotomy were evaluated clinically, radiologically, and histologically during a 3‐year follow‐up. Among DEGs, osteogenesis‐related genes, including BMP1/2, ALP, BGLAP, SP7, RUNX2, SPP1, and EGFR, which play important roles in osteogenesis, were found to be upregulated. The genes to cartilage condensation SOX9, BMPR1B, and osteoclast cells TNFSF11 were detected upregulated at every time point of the investigation. This scaffold has a high osteoinductivity revealed by the matrix mineralization, ALP activity, OCN, and CLEC3B expression proteins. Clinical evaluation evidences that the biomaterial promotes bone regrowth. Histological results of biopsy specimens from patients showed prominent ossification. Experimental data using the Coll/Pro Osteon 200 indicate that clinical evaluation of bone regrowth in patients, after scaffold implantation, was supported by DEGs implicated in skeletal development as shown in “in vitro” experiments with hASCs.

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“…Similarly, the effects of MSCs on osteoclast also depends on the dose of the surrounding biomolecules as well as the concentration of the inflammatory condition [75]. Osteoclast formation was enhanced when cocultured with BM-MSCs in the presence of 10 -9 M 1α,25(OH)2D3.…”

Section: Effects Of Oral Mscs Onmentioning

confidence: 99%

Oral Mesenchymal Stem/Progenitor Cells: The Immunomodulatory Masters

Zhou

Liu

et al. 2020

Stem Cells International

103

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Oral mesenchymal stem/progenitor cells (MSCs) are renowned in the field of tissue engineering/regeneration for their multilineage differentiation potential and easy acquisition. These cells encompass the periodontal ligament stem/progenitor cells (PDLSCs), the dental pulp stem/progenitor cells (DPSCs), the stem/progenitor cells from human exfoliated deciduous teeth (SHED), the gingival mesenchymal stem/progenitor cells (GMSCs), the stem/progenitor cells from the apical papilla (SCAP), the dental follicle stem/progenitor cells (DFSCs), the bone marrow mesenchymal stem/progenitor cells (BM-MSCs) from the alveolar bone proper, and the human periapical cyst-mesenchymal stem cells (hPCy-MSCs). Apart from their remarkable regenerative potential, oral MSCs possess the capacity to interact with an inflammatory microenvironment. Although inflammation might affect the properties of oral MSCs, they could inversely exert a multitude of immunological actions to the local inflammatory microenvironment. The present review discusses the current understanding about the immunomodulatory role of oral MSCs both in periodontitis and systemic diseases, their “double-edged sword” uniqueness in inflammatory regulation, their affection of the immune system, and the underlying mechanisms, involving oral MSC-derived extracellular vesicles.

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The Modulatory Effects of Mesenchymal Stem Cells on Osteoclastogenesis

Cited by 42 publications

References 158 publications

Pro‐inflammatory M1 macrophages promote Osteogenesis by mesenchymal stem cells via the COX‐2‐prostaglandin E2 pathway

Pro‐inflammatory M1 macrophages promote Osteogenesis by mesenchymal stem cells via the COX‐2‐prostaglandin E2 pathway

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

Oral Mesenchymal Stem/Progenitor Cells: The Immunomodulatory Masters

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