The Moisturizing Efficacy of a Dermo-Cosmetic Product (cls02021) Versus in a 6-week Application Period

Abstract

Background: Studies suggest that applying probiotic skincare products may have beneficial effects on the skin due to bacteria competitiveness and specific metabolites produced by probiotics. The cream CLS02021 is a postbiotic blend of metabolites, including organic acids, enzymes, and peptides that are a result of the co-fermentation of three proprietary probiotic strains and is specific for its high concentration of the enzyme Sphingomyelinase which plays a significant role in cell signaling pathways and ceramide production. Objective: The aim of this study is to evaluate the cosmetic effects of probiotic-derived Ingredient CLS02021 and its safety in healthy volunteers. Methods: Fifty healthy volunteers between 18 and 69 years old were recruited to participate in this multicentric, randomized, intra-individual, double-blind group study. Following a face-split design, volunteers applied both face cream containing CLS02021 on one side of their face and a placebo cream on the other side of their face for a total duration of four weeks, two times a day. Evaluation of the cosmetic effects included instrument measurements of moisturizing, elasticity, wrinkle depth, sebum production, pore size, melanin production, sensitivity, and side effects. Results: A significant difference of CLS02021 over the placebo group was observed for moisture and elasticity increase (both p<0.001), pore size (p<0.01), and a wrinkle depth decrease (p<0.05). No significant differences were noted in sebum production, melanin level, skin cleanliness, and sensitivity between groups. Conclusion: CLS02021, as a probiotic-derived ingredient, showed strong beneficial effects on skin moisture and elasticity increase, wrinkle depth, and pore size reduction. Face cream containing CLS02021 is well tolerated and could be an organic choice for cosmetic hydrating and antiaging skincare treatment.

Innovative Approaches for Maintaining and Enhancing Skin Health and Managing Skin Diseases through Microbiome-Targeted Strategies

“…A cell-free supernatant of fermented milk from the L. helveticus strain demonstrated substantial antioxidant capabilities against UVB-induced skin damage, including decreasing lipid peroxidation and inhibiting melanin formation [132]. A customized blend of probiotic strains (Lactobacillus plantarum, Lactobacillus casei, and S. thermophilus) has shown promise in reducing pore size and wrinkle depth, as well as maintaining hydration and skin smoothness [134]. S. thermophiles lysate with sphingomyelinase has been demonstrated to strengthen the lipid barrier of the skin, elevate stratum corneum ceramide levels, and lower water loss [135].…”

Oral and Topical Probiotics and Postbiotics in Skincare and Dermatological Therapy: A Concise Review

“…In addition, reduction of transepidermal water loss, increase of epidermal thickness, and favorably modulated lipid peroxidation levels were observed [ 120 ]. A blend resulting from the co-fermentation of three proprietary probiotic strains, L. plantarum (AN057), L. casei (AN177), and S. thermophilus (AN157), showed beneficial effects on pore size and wrinkle depth reduction, as well as skin moisture and elasticity increase [ 121 ].…”

Anti-Inflammation and Anti-Melanogenic Effects of Maca Root Extracts Fermented Using Lactobacillus Strains

“…Additionally, a probiotic-derived ingredient (CLS02021)—a postbiotic mixture of metabolites including enzymes, organic acids, and peptides resulting from the co-fermentation of three probiotic strains—has contributed to increasing the elasticity, moisture, pore-size reduction, and wrinkle depth of the skin. Similarly, heat-killed Lactococcus chungagnensis CAU 1447 has shown beneficial effects on wound healing by inducing the expression of wound-healing-promoting cytokines, chemokines, and growth factors [ 15 , 16 ]. This suggests that the topical application of postbiotics has beneficial effects on the skin; consequently, there is a growing market trend for the inclusion of postbiotics in cosmetic ingredients and/or products [ 17 ].…”