The molecular basis of anaphase A in animal cells

Rath, Uttama; Sharp, David J.

doi:10.1007/s10577-011-9199-2

Cited by 12 publications

(11 citation statements)

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“…In Drosophila cells, KLP10A inhibition results in an almost complete suppression of poleward flux [31]. To assess whether Kin-Tub-2 mutations affect the ability of kinesin-13s to depolymerize MTs in-vivo , we measured the anaphase-A poleward flux rates of the cell groups described in the previous section using fluorescent live cell imaging (Figure 5, Movie S2).…”

Section: Resultsmentioning

confidence: 99%

A Second Tubulin Binding Site on the Kinesin-13 Motor Head Domain Is Important during Mitosis

et al. 2013

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Kinesin-13s are microtubule (MT) depolymerases different from most other kinesins that move along MTs. Like other kinesins, they have a motor or head domain (HD) containing a tubulin and an ATP binding site. Interestingly, kinesin-13s have an additional binding site (Kin-Tub-2) on the opposite side of the HD that contains several family conserved positively charged residues. The role of this site in kinesin-13 function is not clear. To address this issue, we investigated the in-vitro and in-vivo effects of mutating Kin-Tub-2 family conserved residues on the Drosophila melanogaster kinesin-13, KLP10A. We show that the Kin-Tub-2 site enhances tubulin cross-linking and MT bundling properties of KLP10A in-vitro. Disruption of the Kin-Tub-2 site, despite not having a deleterious effect on MT depolymerization, results in abnormal mitotic spindles and lagging chromosomes during mitosis in Drosophila S2 cells. The results suggest that the additional Kin-Tub-2 tubulin biding site plays a direct MT attachment role in-vivo.

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Section: Resultsmentioning

confidence: 99%

A Second Tubulin Binding Site on the Kinesin-13 Motor Head Domain Is Important during Mitosis

et al. 2013

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“…This was a truly synergistic effect and did not occur in cells lacking either Aurora A or B activity, but only after inactivation of both kinases. Rapid depolymerization of K-fibre MTs is thought to be a major contributor to force generation in spindle in anaphase that is required to pull the sister chromatids apart [9,103]. MT depolymerization can occur at both the MT plus and minus ends (figure 2 c ).…”

Section: Overlapping Roles Of Centromeric and Centrosomal Aurora Kinasesmentioning

confidence: 99%

Aurora at the pole and equator: overlapping functions of Aurora kinases in the mitotic spindle

2013

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The correct assembly and timely disassembly of the mitotic spindle is crucial for the propagation of the genome during cell division. Aurora kinases play a central role in orchestrating bipolar spindle establishment, chromosome alignment and segregation. In most eukaryotes, ranging from amoebas to humans, Aurora activity appears to be required both at the spindle pole and the kinetochore, and these activities are often split between two different Aurora paralogues, termed Aurora A and B. Polar and equatorial functions of Aurora kinases have generally been considered separately, with Aurora A being mostly involved in centrosome dynamics, whereas Aurora B coordinates kinetochore attachment and cytokinesis. However, double inactivation of both Aurora A and B results in a dramatic synergy that abolishes chromosome segregation. This suggests that these two activities jointly coordinate mitotic progression. Accordingly, recent evidence suggests that Aurora A and B work together in both spindle assembly in metaphase and disassembly in anaphase. Here, we provide an outlook on these shared functions of the Auroras, discuss the evolution of this family of mitotic kinases and speculate why Aurora kinase activity may be required at both ends of the spindle microtubules.

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“…In this article, we describe an unconventional variation of anaphase A in which spindle poles move inward toward stationary chromosomes. Conventional anaphase A is driven by the shortening of microtubule bundles that have plus ends terminating at kinetochores and minus ends terminating at the spindle pole ( Rath and Sharp, 2011 ). Attachment of chromosomes to the depolymerizing plus ends is mediated by the microtubule-binding components of the outer kinetochore, the NDC80 complex and Kinetochore NulI (KNL-1; Cheeseman et al ., 2006 ).…”

Section: Introductionmentioning

confidence: 99%