The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea

Johzuka-Hisatomi, Yasuyo; Noguchi, Hiroshi; Iida, Shigeru

doi:10.1007/s10265-010-0369-7

Cited by 9 publications

(5 citation statements)

References 23 publications

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“…Incompletely dominant petal pigmentation has been reported and well characterized in snapdragon (Niv locus) and morning glory (A locus) (Bollmann et al, 1991;Coen and Carpentaer, 1988;Johzuka-Hisatomi et al, 2010). Both the Niv and the A loci encode chalcone synthase (CHS).…”

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confidence: 99%

“…Similar pigmentation was found for the style color (B). ate pigmentation is caused by a decrease in the level of the CHS transcript, whose expression level in A/a petals is about half of that in A/A petals (Bollmann et al, 1991;Coen and Carpentaer, 1988;Johzuka-Hisatomi et al, 2010). An anthocyanidin triglycoside has been isolated from L. grandiflorum red petals as the main anthocyanin, and the pigment structure is delphinidin 3-O-3-xylosylrutinoside 78 (Toki et al, 1995).…”

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Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

et al. 2015

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Two types of floral morph, called thrum and pin, occur in heterostylous species. The thrum and pin flowers have shorter and longer styles, respectively. Heterostyly in Linum has been studied since Darwin's era. The floral morph, self-incompatibility, and related phenotypes have been well characterized using a natural population, but genetic analysis using a segregated population has not been reported, and the mode of inheritance of heterostyly in Linum remains to be investigated. We prepared a segregated population by crossing thrum and pin flowers of Linum grandiflorum Desf. and investigated style and stamen lengths. On the basis of the style to stamen length ratio, the population could be divided into thrum and pin clearly at a ratio of 1:1. Style length of pins was 1.6 times longer than that of thrums. To investigate the factor regulating the difference in style length, we further measured the style cell length of thrums and pins. The style cell length of pins was longer than that of thrums, whose rate was comparable to the rate of the style length ratio between thrum and pin flowers. These findings indicate that the floral morph of heterostyly in Linum is controlled by a single diallelic locus, and that a difference in the cell expansion rate caused thrum and pin morphs in the style, such as in a typical heterostylous species. PCR genotyping showed that TSS1, an S candidate gene reported previously, cosegregated completely with the thrum phenotype, indicating strong linkage between the S locus and TSS1. Furthermore, three flower colors (red, pink, and white) were observed at a 1:2:1 ratio, and no white thrum flowers or red pin flowers were found in this population. These flower color phenotypes could also be controlled by a diallelic locus, whose two alleles (R and r) would be incompletely dominant, and therefore, may be linked to the S locus.

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Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

et al. 2015

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“…This apparent discrepancy can be explained by assuming that these ordinary genes are generally expressed more than twice as much as the necessary levels in the wild type. Consistent to this notion, dosage-dependent expression of a certain gene is the primary cause of the observed incomplete dominance (Johzuka-Hisatomi et al 2011 ). Because the knockin-targeting strategy allows detection of spatiotemporal expression of the target gene, utilization of other more sensitive markers such as green fl uorescent protein, in combination with other available techniques including fl uorescence-activated cell sorting, would facilitate further detailed characterization of the target gene function through in vivo analysis and/or isolation of viable tissues expressing the target gene (Ono et al 2012 ).…”

Section: Knockout and Knockin Targetingmentioning

confidence: 77%

Advances in New Technology for Targeted Modification of Plant Genomes

Zhang

Puchta

Thomson³

2015

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“…1 ). In I. purpurea , an I. nil- related morning glory, dosage-dependent expression of the CHS gene led to the incomplete dominance of flower coloration ( Johzuka-Hisatomi et al 2011 ), suggesting that the reduction of CHS activity confers pale-colored flowers in I. nil . Incomplete dominance has not been reported in other genes in the early steps.…”

Section: A Novel Color Intensifier Efp (Enhancer Of Flavonoid Producmentioning

confidence: 99%

Recent advances in flower color variation and patterning of Japanese morning glory and petunia

Morita

Hoshino

2018

Breed. Sci.

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The Japanese morning glory (Ipomoea nil) and petunia (Petunia hybrida), locally called “Asagao” and “Tsukubane-asagao”, respectively, are popular garden plants. They have been utilized as model plants for studying the genetic basis of floricultural traits, especially anthocyanin pigmentation in flower petals. In their long history of genetic studies, many mutations affecting flower pigmentation have been characterized, and both structural and regulatory genes for the anthocyanin biosynthesis pathway have been identified. In this review, we will summarize recent advances in the understanding of flower pigmentation in the two species with respect to flower hue and color patterning. Regarding flower hue, we will describe a novel enhancer of flavonoid production that controls the intensity of flower pigmentation, new aspects related to a flavonoid glucosyltransferase that has been known for a long time, and the regulatory mechanisms of vacuolar pH being a key determinant of red and blue coloration. On color patterning, we describe particular flower patterns regulated by epigenetic and RNA-silencing mechanisms. As high-quality whole genome sequences of the Japanese morning glory and petunia wild parents (P. axillaris and P. inflata, respectively) were published in 2016, further study on flower pigmentation will be accelerated.

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The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea

Cited by 9 publications

References 23 publications

Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

Genetic Control of Floral Morph and Petal Pigmentation in <i>Linum grandiflorum</i> Desf., a Heterostylous Flax

Advances in New Technology for Targeted Modification of Plant Genomes

Recent advances in flower color variation and patterning of Japanese morning glory and petunia

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