The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

Frandsen, Kristian E. H.; Simmons, Thomas J.; Dupree, Paul; Poulsen, Jens-Christian N.; Hemsworth, G.R.; Ciano, Luisa; Johnston, Esther M.; Tovborg, Morten; Johansen, Katja Salomon; Freiesleben, Pernille von; Marmuse, Laurence; Fort, Sébastien; Cottaz, Sylvain; Driguez, Hugues; Henrissat, Bernard; Lenfant, Nicolas; Tuna, Floriana; Baldansuren, Amgalanbaatar; Davies, G.J.; Leggio, Leila Lo; Walton, Paul H.

doi:10.1038/nchembio.2029

Cited by 286 publications

(567 citation statements)

References 51 publications

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“…The flat catalytic binding surfaces of LPMOs are well suited to bind polysaccharides and disrupt glycosidic linkages (38). However, a few LPMOs are characterized to bind and cleave shorter cellooligosaccharides such as cellohexaose and celloheptaose (39) whereas oligosaccharides smaller than cellohexaose cannot be accommodated in LPMO flat binding site (40). This suggests that LPMO bind and cleave higher oligomers and crystalline surfaces, thereby acting as first wave of attack in disintegration of recalcitrant biomass.…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Identification of an endogenous redox partner for lytic polysaccharide monooxygenase-based oxidative cleavage of polysaccharides

2017

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Identification of an endogenous redox partner for lytic polysaccharide monooxygenase-based oxidative cleavage of polysaccharides

2017

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“…Accordingly, LPMOs have been at the centre of much research attention, with several recent reviews providing insights into the published data and the possible mechanisms of action of LPMOs [7][8][9][10][11] , including a very recent structure of an LPMO in association with an oligosaccharide substrate 12 . LPMOs use an oxidative mechanism to introduce chain breaks into polysaccharides thereby augmenting the activity of classical glycoside hydrolases, as shown by Vaaje Kolstad et al 13 in their breakthrough study of CBP21 (chitin-binding protein 21) from Serratia marcescens.…”

Section: Introductionmentioning

confidence: 99%

Activity, stability and 3-D structure of the Cu(ii) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens

et al. 2016

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The enzymatic deconstruction of recalcitrant polysaccharide biomass is central to the conversion of these substrates for societal benefit, such as in biofuels. Traditional models for enzyme-catalysed polysaccharide degradation involved the synergistic action of endo-, exo-and processive glycoside hydrolases working in concert to hydrolyse the substrate. More recently this model has been succeeded by one featuring a newly discovered class of mononuclear copper enzymes: lytic polysaccharide monooxygenases (LPMOs; classified as Auxiliary Activity (AA) enzymes in the CAZy classification). In 2013, the structure of an LPMO from Bacillus amyloliquefaciens, BaAA10, was solved with the Cu centre photoreduced to Cu(I) in the X-ray beam. Here we present the catalytic activity of BaAA10. We show that it is a chitin-active LPMO, active on both α and β chitin, with the Cu(II) binding with low nM KD, and the substrate greatly increasing the thermal stability of the enzyme. A spiral data collection strategy has been used to facilitate access to the previously unobservable Cu(II) state of the active centre, revealing a coordination geometry around the copper which is distorted from axial symmetry, consistent with the previous findings from EPR spectroscopy.

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“…The utmost levels of total sugar content were found for treatments 21/15/9ºC and 15/9/6ºC, both with a significant difference to the lower level at 21ºC [42]. Shattuck et al (1991a) reported that a low growth temperature (0-12ºC) for 11 days before harvesting considerably increased the total sugar content [39][40][41][42][43]. Comparable results have also been reported by Davik (1992) and Shattuck et al (1991a).…”

Section: Discussionmentioning

confidence: 87%

“…Conversely, Molmann et al (2015) found no significant differences in vitamin C content between broccoli grown at 12ºC and 18ºC [41]. The utmost levels of total sugar content were found for treatments 21/15/9ºC and 15/9/6ºC, both with a significant difference to the lower level at 21ºC [42]. Shattuck et al (1991a) reported that a low growth temperature (0-12ºC) for 11 days before harvesting considerably increased the total sugar content [39][40][41][42][43].…”

Section: Discussionmentioning

confidence: 97%

Differential Effect of Day and Night Temperature Regimes on the Growth and Biochemical Attributes of Violet Rape (Brassica campestris ssp. chinensis L.)

Zhao¹,

Muhammad²,

Bilal³

et al. 2018

Pol. J. Environ. Stud.

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The present study investigated the influences of different day and night temperature regimes on the growth of violet rape (Brassica rapa var. chinensis) and pinpointed the optimal temperature combination for both yield and quality of the plant. For this purpose, the plants were grown under 6 temperature combination conditions: W1 (30/20ºC, 12-h light/12-h dark), W2 (25/15ºC, 12-h light/12-h dark), W3 (20/10ºC, 12-h light/12-h dark), W4 (30/15°C, 12-h light/12-h dark), W5 (25/10ºC, 12-h light/12-h dark), and W6 (30/10ºC, 12-h light/12-h dark), and in addition we measured various morphological, yield, and biochemical traits. Results demonstrated that temperature regimes considerably affected the growth parameters of the violet rape compared with control. The W3 treatment with an average temperature of 20/10ºC displayed the highest plant growth traits, including plant height (12.74±0.24 cm), leaf length (8.20±0.08 cm), leaf width (5.44±0.16 cm), and leaf area (33.46±0.83 cm²). Moreover, a highly significant correlation (p<0.05) was found between temperature and soluble protein, chlorophyll, anthocyanin, nitrate nitrogen contents, and root activity; whereas the vitamin C and soluble sugar contents remained significantly unaffected except in the W1 treatment, under different temperature combinations between day and night. This study concluded that high temperature exerts an adverse effect on plant growth, and therefore a low day and night temperature combination is suggested in order to achieve high yield and quality for irrigated violet rape.

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The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases

Abstract: International audienceno abstrac

Cited by 286 publications

References 51 publications

WITHDRAWN: Identification of an endogenous redox partner for lytic polysaccharide monooxygenase-based oxidative cleavage of polysaccharides

WITHDRAWN: Identification of an endogenous redox partner for lytic polysaccharide monooxygenase-based oxidative cleavage of polysaccharides

Activity, stability and 3-D structure of the Cu(ii) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens

Differential Effect of Day and Night Temperature Regimes on the Growth and Biochemical Attributes of Violet Rape (Brassica campestris ssp. chinensis L.)

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