2014
DOI: 10.1002/elps.201400141
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The molecular characterization of a depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics

Abstract: The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any … Show more

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Cited by 12 publications
(27 citation statements)
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“…To enrich the samples in A‐P sites, a method previously published was followed . Briefly, 200 μL aliquots, each one containing about 13 μg of DNA, were incubated in a water bath at 70°C for 6.0, 8.0 and 10.0 hours (namely sample FM‐6, FM‐8 and FM‐10, respectively).…”
Section: Methodsmentioning
confidence: 99%
“…To enrich the samples in A‐P sites, a method previously published was followed . Briefly, 200 μL aliquots, each one containing about 13 μg of DNA, were incubated in a water bath at 70°C for 6.0, 8.0 and 10.0 hours (namely sample FM‐6, FM‐8 and FM‐10, respectively).…”
Section: Methodsmentioning
confidence: 99%
“…In fact, the ability of calling the STR loci decreased from 46 to 17% proportionally to the degradation of the trial samples and similarly from 68 to 26% for the SNP loci. In addition, correct typing according to the interpretation guidelines described in [5] was achieved from 39 to 4% of the STRs and from 55 to 13% of the SNPs when the genotypes from the trial samples were compared to the ones recovered from the unmodified control DNA (see Fig. 1).…”
Section: Resultsmentioning
confidence: 99%
“…1. Evaluation of the genotypes according to [5]. Y axis: frequency of the loci correctly typed (C), of the loci which gave no results (NR), ambiguous (U) or wrong typing (W).…”
Section: Discussionmentioning
confidence: 99%
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