The molecular detection of Fusarium oxysporum f. sp. dianthi races in protected carnation growing areas in western Turkey

Cer, Ceren; Benlioğlu, Seher; Eğerci, Yeşim

doi:10.1007/s41348-021-00545-9

Cited by 3 publications

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“…& Delacr.) W. C. Snyder & H. N. Hansen ( Fod ) is the most serious problem for carnations in Türkiye (Cer et al., 2021) and worldwide. Considering the contribution of the export of cut flower carnations to the Turkish economy and the extent of the damage caused by Fusarium wilt on carnations, a comprehensive survey was carried out in the carnation greenhouses on the western coast of Türkiye.…”

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confidence: 99%

“…Disease Severity Indexes (DSI) of Fp isolates were calculated. According to the DSIs, isolates were separated into three groups; highly virulent (≥70%), moderately virulent (35%–70%), and weakly virulent (≤35%) categories (Cer et al., 2021).…”

Section: Figurementioning

confidence: 99%

“…isolates were obtained from 92 carnation greenhouses, 80 had been identified as Fusarium oxysporum f.sp. dianthi and published earlier (Cer et al, 2021). In this study, 15 Fp isolates were obtained from wilted carnations in different carnation-growing greenhouses in İzmir and identified based on morphological and molecular analysis using TEF1-α gene sequences.…”

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confidence: 99%

“…& Delacr.) W. C. Snyder & H. N. Hansen (Fod) is the most serious problem for carnations in Türkiye (Cer et al, 2021) and worldwide.…”

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confidence: 99%

“…Recently, Fp has been frequently found in association with wilt diseases on annual crops such as asparagus (Elmer, 1990) in USA, sesame (Torabi et al, 2014) in Iran, safflower in Korea (Kim et al, 2016, alfalfa in China (Cong et al, 2016), cauliflower in China (Yan, 2020), strawberry in Spain (Borrero et al, 2019), cowpea in Brazil (Farias et al, 2022), and perennials as date palm reported in Tunisia (Namsi et al, 2020), in Jordan (Alananbeh et al, 2021) and Iran (Ghaedi et al, 2021).…”

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Occurrence of wilt disease caused by Fusarium proliferatum on carnation in Türkiye

Cer,

Benlioglu

2023

Journal of Phytopathology

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During the survey in greenhouse‐cut flower carnation (Dianthus caryophyllus L.) areas in Izmir, wilted carnation samples were collected from 92 greenhouses. A total of 95 Fusarium spp. isolates were obtained from carnation greenhouses, 80 had been identified as Fusarium oxysporum f.sp. dianthi and published earlier, while the remaining 15 were identified as Fusarium proliferatum (Fp) based on morphological and molecular analysis using TEF1‐α gene sequences in this study. The prevalence of wilting disease of carnation caused by Fp was estimated to be 16.3% of the total area. Pathogenicity tests were conducted by dipping the carnation roots (cv. Turbo) in a spore suspension of monoconidial isolates and transplanting them into pots. Pathogenicity studies confirmed the ability of all Fp to cause symptoms of wilting, leaves and stems shriving. Phylogenetic analysis of sequences from the translation elongation factor 1α (TEF‐1 α) region of the 15 Fp isolates obtained from carnation formed a large clade of isolates originating from carnation and a wide range of other hosts in different geographical locations worldwide. To our knowledge, this is the first report of F. proliferatum causing wilt disease of carnation based on molecular identification in Türkiye.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

mentioning

confidence: 99%

“…& Delacr.) W. C. Snyder & H. N. Hansen (Fod) is the most serious problem for carnations in Türkiye (Cer et al, 2021) and worldwide.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Occurrence of wilt disease caused by Fusarium proliferatum on carnation in Türkiye

Cer,

Benlioglu

2023

Journal of Phytopathology

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Molecular characterization and biocontrol potential of Trichoderma spp. against Fusarium oxysporum f. sp. dianthi in carnation

Zandyavari,

Sulaiman,

Hassanzadeh

2024

Egypt J Biol Pest Control

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Background Carnation, a major cut flower product cultivated economically in Iran, faces economic challenges due to the devastating Fusarium wilt caused by Fusarium oxysporum f. sp. dianthi (Fod). To address this issue, twenty-five Trichoderma harzianum and T. viride isolates were collected from the rhizosphere soil of three Iranian provinces: Tehran, Markazi, and Fars. RAPD-PCR was applied to analyze the genetic relatedness of the isolates. Results The RAPD profiles showed genetic diversity among the isolates, with two major clusters. The antagonistic potential of the twenty isolates was evaluated against the carnation wilt caused by Fod. The results showed that Th1, Th7, and Th2 isolates of T. harzianum significantly inhibited Fod mycelial growth (58, 56.5, and 48.6%, respectively). Among T. viride isolates, Tv5, Tv4, and Tv7 exhibited the highest antifungal ability to inhibit mycelial growth. All investigated isolates of Trichoderma secreted volatile compounds that hindered Fod mycelial growth, with isolates of T. harzianum ranging from 9.3 to 67.5% inhibition and those of T. viride from 25.2 to 50.2%. Additionally, the experiment on competitive saprophytic ability indicated that maximum colonization occurred with Th1, Th7, and Th2 isolates at 78.2, 70.8, and 69.8%, respectively. Lastly, the greenhouse experiment showed a complete pathogen eradication or significant inhibition in the infected carnation after T. harzianum and T. viride. Conversely, control treatment with the Fod pathogen died after 90 days. Conclusions The investigation suggested that Trichoderma spp. could be a potential biocontrol agent to mitigate Fusarium wilt in carnation and improve production quality, replacing chemical pesticides.

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Study on Genetic Diversity of Fusarium oxysporum f.sp. dianthi Based on Vegetative Compatibility Groups and Races in Five Provinces of Iran

ZANDYAVARİ>¹,

Bayat

Hasanzadeh

2022

Değerlendirme süreci

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Fusarium wilt of carnation caused by Fusarium oxysporum f.sp. dianthi (Fod) W.C.Snyder & H.N. Hensen, is the most important fungal disease in all main carnation growing regions of Iran. In 2020, sampling of infected plants was randomly carried out from carnation greenhouses in five provinces located in the North (Gilan, Golestan, and Mazandaran) and Middle (Tehran and Markazi) of Iran. Eighty-eight fungal isolates were identified as Fod by morphological characters (based on Nelson’s key identification), molecular method (PCR amplified of ITS region), and pathogenicity tests (according to the root dipping on sensitive cv. Rendezvous). In order to investigate on biodiversity of Iranian Fod isolates based on Vegetative Compatibility Groups (VCGs) and races, 70 isolates were randomly selected. These isolates were compared with eight Fod isolates from the collection of Dipartimento di Valorizzazione e Protezione delle Risorse Agroforestali (Di.Va.P.R.A) Patologia vegetale, Italy; which were used as references for races and VCGs. For DNA amplifying, primers R 1.8, R 2.1, and R 4.2 were employed. Amplified fragments of 295 bp and 564 bp were identified to race 1 (or 8; because these primers applied are not able to discriminate between the races), and race 2 (or 5 or 6), respectively. Furthermore, two VCG 0021 and VCG 0022 were identified amongst the Iranian Fod isolates. The relationship between the races and VCGs demonstrated that all race 1 (23 isolates) were categorized in VCG 0022, and race 2 (44 isolates) were grouped in VCG 0021. In addition, three isolates did not produce any amplification.

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The molecular detection of Fusarium oxysporum f. sp. dianthi races in protected carnation growing areas in western Turkey

Cited by 3 publications

References 27 publications

Occurrence of wilt disease caused by Fusarium proliferatum on carnation in Türkiye

Occurrence of wilt disease caused by Fusarium proliferatum on carnation in Türkiye

Molecular characterization and biocontrol potential of Trichoderma spp. against Fusarium oxysporum f. sp. dianthi in carnation

Study on Genetic Diversity of Fusarium oxysporum f.sp. dianthi Based on Vegetative Compatibility Groups and Races in Five Provinces of Iran

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