The molecular diversity of transcriptional factor TfoX is a determinant in natural transformation in Glaesserella parasuis

Tang, Xiaoyu; Yang, Zhen; Dai, Ke; Liu, Geyan; Chang, Yung-Fu; Tang, Xinwei; Wang, Kang; Zhang, Yiwen; Hu, Bangdi; Cao, Sanjie; Huang, Xiaobo; Yan, Qigui; Wu, Rui; Zhao, Qin; Du, Senyan; Lang, Yifei; Han, Xinfeng; Huang, Yong; Wen, Xintian; Wen, Yiping

doi:10.3389/fmicb.2022.948633

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…Refer to the previous procedure for qRT-PCR [48]. RNA from PK15 cells incubated with EspP2 was isolated using a UNIQ-10 Column Total RNA Purification Kit (Sangon, China).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

EspP2 Regulates the Adhesion of Glaesserella parasuis via Rap1 Signaling Pathway

Tang,

Xu,

Yang

et al. 2024

IJMS

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Different levels of EspP2 expression are seen in strains of Glaesserella parasuis with high and low pathogenicity. As a potential virulence factor for G. parasuis, the pathogenic mechanism of EspP2 in infection of host cells is not clear. To begin to elucidate the effect of EspP2 on virulence, we used G. parasuis SC1401 in its wild-type form and SC1401, which was made EspP2-deficient. We demonstrated that EspP2 causes up-regulation of claudin-1 and occludin expression, thereby promoting the adhesion of G. parasuis to host cells; EspP2-deficiency resulted in significantly reduced adhesion of G. parasuis to cells. Transcriptome sequencing analysis of EspP2-treated PK15 cells revealed that the Rap1 signaling pathway is stimulated by EspP2. Blocking this pathway diminished occludin expression and adhesion. These results indicated that EspP2 regulates the adhesion of Glaesserella parasuis via Rap1 signaling pathway.

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“…Refer to the previous procedure for qRT-PCR [48]. RNA from PK15 cells incubated with EspP2 was isolated using a UNIQ-10 Column Total RNA Purification Kit (Sangon, China).…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

EspP2 Regulates the Adhesion of Glaesserella parasuis via Rap1 Signaling Pathway

Tang,

Xu,

Yang

et al. 2024

IJMS

Self Cite

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Upregulation of occludin by cytolethal distending toxin facilitates Glaesserella parasuis adhesion to respiratory tract cells

Yang,

Zhang,

et al. 2023

Infect Immun

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Virulent Glaesserella parasuis may engender systemic infection characterized by fibrinous polyserositis and pneumonia. G. parasuis causes systemic disease through upper respiratory tract infection, but the mechanism has not been fully characterized. Tight junction (TJ) proteins maintain the integrity and impermeability of the epithelial barriers. In this work, we applied the recombinant cytolethal distending toxin (CDT) holotoxin and cdt -deficient mutants to assess whether CDT interacted with TJ proteins of airway tract cells. Our results indicated that CDT induced the TJ occludin (OCLN) expression in newborn pig tracheal epithelial cells within the first 3 hours of bacterial infection, followed by a significant decrease. Overexpression of OCLN in target cells made them more susceptible to G. parasuis adhesion, whereas ablation of OCLN expression by CRISPR/Cas 9 gene editing technology in target cells decreased their susceptibility to bacterial adhesion. In addition, CDT treatment could upregulate the OCLN levels in the lung tissue of C57/BL6 mice. In summary, highly virulent G. parasuis strain SC1401 stimulated the tight junction expression, resulting in higher bacterial adhesion to respiratory tract cells, and this process is closely related to CDT. Our results may provide novel insights into G. parasuis infection and CDT-mediated pathogenesis.

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HbpA from Glaesserella parasuis induces an inflammatory response in 3D4/21 cells by activating the MAPK and NF-κB signalling pathways and protects mice against G. parasuis when used as an immunogen

Yang,

Zhang,

Zhao

et al. 2024

Vet Res

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Glaesserella parasuis is usually a benign swine commensal in the upper respiratory tract, but virulent strains can cause systemic infection characterized by pneumonia, meningitis, and fibrinous polyserositis. The intensive pulmonary inflammatory response following G. parasuis infection is the main cause of lung injury and death in pigs. Vaccination has failed to control the disease due to the lack of extended cross-protection. Accumulating evidence indicates that the heme-binding protein A (HbpA) is a potential virulence determinant and a promising antigen candidate for the development of a broader range of vaccines. However, it is not yet known whether HbpA contributes to G. parasuis virulence or has any potential immune protective effects against G. parasuis. Here, we show that HbpA can induce the transcription and secretion of proinflammatory cytokines (IL-6, TNF-α, and MCP-1) in porcine alveolar macrophages (PAM, 3D4/31). The HbpA protein is recognized by Toll-like receptors 2 and 4 on 3D4/21 macrophages, resulting in the activation of MAP kinase and NF-κB signalling cascades and the transcription and secretion of proinflammatory cytokines. HbpA contributes to virulence and bacterial pulmonary colonization in C57BL/6 mice and plays a role in adhesion to host cells and evasion of the bactericidal effect of pulmonary macrophages. In addition, mice immunized with HbpA were partially protected against challenge by G. parasuis SC1401. The results suggest that HbpA plays an important role in the pathogenesis of disease caused by G. parasuis and lay a foundation for the development of a subunit or chimeric anti-G. parasuis vaccine.

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The molecular diversity of transcriptional factor TfoX is a determinant in natural transformation in Glaesserella parasuis

Cited by 3 publications

References 61 publications

EspP2 Regulates the Adhesion of Glaesserella parasuis via Rap1 Signaling Pathway

EspP2 Regulates the Adhesion of Glaesserella parasuis via Rap1 Signaling Pathway

Upregulation of occludin by cytolethal distending toxin facilitates Glaesserella parasuis adhesion to respiratory tract cells

HbpA from Glaesserella parasuis induces an inflammatory response in 3D4/21 cells by activating the MAPK and NF-κB signalling pathways and protects mice against G. parasuis when used as an immunogen

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