The molecular genetic linkage map of the model legume Medicago truncatula: an essential tool for comparative legume genomics and the isolation of agronomically important genes

Thoquet, P.; Ghérardi, M.; Journet, Etienne-Pascal; Kereszt, Attila; Ané, Jean‐Michel; Prosperi, J.M.; Huguet, Thierry

doi:10.1186/1471-2229-2-1

Cited by 169 publications

(31 citation statements)

References 74 publications

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“…Under such stressful conditions, DHNs accumulate in most tissues and cells and are mainly distributed in the nucleus and cytoplasm of the plant cell. Previous studies indicated that DHNs interact with the chloroplast (Mueller and Fernando, 2003; Tunnacliffe and Wise, 2007), mitochondria (Borovskii et al, 2002; Grelet, 2004), tonoplast (Heyen et al, 2002), endoplasmic reticulum (ER) (Ukaji et al, 2001), cytosol (Roberts et al, 1993), and nuclei (Liu et al, 2013). Some DHNs were found to accumulate in the root tip, open stomata, and cells surrounding the vascular tissue, suggesting that these proteins may play roles in these special tissues under non-stress conditions (Nylander et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Physcomitrella Patens Dehydrins (PpDHNA and PpDHNC) Confer Salinity and Drought Tolerance to Transgenic Arabidopsis Plants

Zhang

et al. 2017

Front. Plant Sci.

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Dehydrins (DHNs) as a member of late-embryogenesis-abundant (LEA) proteins are involved in plant abiotic stress tolerance. Two dehydrins PpDHNA and PpDHNC were previously characterized from the moss Physcomitrella patens, which has been suggested to be an ideal model plant to study stress tolerance due to its adaptability to extreme environment. In this study, functions of these two genes were analyzed by heterologous expressions in Arabidopsis. Phenotype analysis revealed that overexpressing PpDHN dehydrin lines had stronger stress resistance than wild type and empty-vector control lines. These stress tolerance mainly due to the up-regulation of stress-related genes expression and mitigation to oxidative damage. The transgenic plants showed strong scavenging ability of reactive oxygen species(ROS), which was attributed to the enhancing of the content of antioxidant enzymes like superoxide dismutase (SOD) and catalase (CAT). Further analysis showed that the contents of chlorophyll and proline tended to be the appropriate level (close to non-stress environment) and the malondialdehyde (MDA) were repressed in these transgenic plants after exposure to stress. All these results suggest the PpDHNA and PpDHNC played a crucial role in response to drought and salt stress.

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Section: Introductionmentioning

confidence: 99%

Physcomitrella Patens Dehydrins (PpDHNA and PpDHNC) Confer Salinity and Drought Tolerance to Transgenic Arabidopsis Plants

Zhang

et al. 2017

Front. Plant Sci.

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“…The first steps towards sequencing were taken when Nam et al (1999) produced the first BAC clones from Jemalong A17. The first published genetic map of M. truncatula was produced by Thoquet et al (2002) using two homozygous lines selected from Jemalong (Jemalong 6 or J6) and the Algerian natural population DZA315. Thoquet et al (2002) noted that the three Jemalong lines A17, J5 and J6 could be considered to have an identical genotype (but different to the highly regenerable Jemalong genotype 2HA).…”

Section: Medicago Truncatula As a Model Legumementioning

confidence: 99%

“…The first published genetic map of M. truncatula was produced by Thoquet et al (2002) using two homozygous lines selected from Jemalong (Jemalong 6 or J6) and the Algerian natural population DZA315. Thoquet et al (2002) noted that the three Jemalong lines A17, J5 and J6 could be considered to have an identical genotype (but different to the highly regenerable Jemalong genotype 2HA). BAC clones were mapped to M. truncatula A17 pachytene chromosomes by fluorescent in situ hybridisation (FISH), (Kulikova et al 2001;Choi et al 2004a;Kulikova et al 2004).…”

Section: Medicago Truncatula As a Model Legumementioning

confidence: 99%

“…Genome conservation between M. truncatula and crop and other legumes, including Lotus japonicus, has been examined (Choi et al 2004b;Cannon et al 2006). There is substantive synteny between M. truncatula and M. sativa (Thoquet et al 2002;Choi et al 2004a) and M. truncatula and Pisum sativum L. (Choi et al 2004b;Aubert et al 2006). These mapping studies will provide valuable information for fundamental research and translational research with crop legumes.…”

Section: Medicago Truncatula As a Model Legumementioning

confidence: 99%

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Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future

Rose¹

2008

Functional Plant Biol.

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Abstract. Medicago truncatula Gaertn. cv. Jemalong, a pasture species used in Australian agriculture, was first proposed as a model legume in 1990. Since that time M. truncatula, along with Lotus japonicus (Regal) Larsen, has contributed to major advances in understanding rhizobia Nod factor perception and the signalling pathway involved in nodule formation. Research using M. truncatula as a model has expanded beyond nodulation and the allied mycorrhizal research to investigate interactions with insect pests, plant pathogens and nematodes. In addition to biotic stresses the genetic mechanisms to ameliorate abiotic stresses such as salinity and drought are being investigated. Furthermore, M. truncatula is being used to increase understanding of plant development and cellular differentiation, with nodule differentiation providing a different perspective to organogenesis and meristem biology. This legume plant represents one of the major evolutionary success stories of plant adaptation to its environment, and it is particularly in understanding the capacity to integrate biotic and abiotic plant responses with plant growth and development that M. truncatula has an important role to play. The expanding genomic and genetic toolkit available with M. truncatula provides many opportunities for integrative biological research with a plant which is both a model for functional genomics and important in agricultural sustainability.

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“…Medicago truncatula (Medicago) is a well-established model for studying legume biology and, in particular, the molecular mechanisms mediating symbiotic associations (Cook, 1999). The Medicago research community has developed many genetic, proteomic, and genomic tools, including the recent release of its genome sequence (Thoquet et al, 2002; Ané et al, 2008; Colditz and Braun, 2010; Young et al, 2011). …”

Section: Introductionmentioning

confidence: 99%

Medicago PhosphoProtein Database: a repository for Medicago truncatula phosphoprotein data

Rose¹,

Venkateshwaran²,

Grimsrud³

et al. 2012

Front. Plant Sci.

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The ability of legume crops to fix atmospheric nitrogen via a symbiotic association with soil rhizobia makes them an essential component of many agricultural systems. Initiation of this symbiosis requires protein phosphorylation-mediated signaling in response to rhizobial signals named Nod factors. Medicago truncatula (Medicago) is the model system for studying legume biology, making the study of its phosphoproteome essential. Here, we describe the Medicago PhosphoProtein Database (MPPD; http://phospho.medicago.wisc.edu), a repository built to house phosphoprotein, phosphopeptide, and phosphosite data specific to Medicago. Currently, the MPPD holds 3,457 unique phosphopeptides that contain 3,404 non-redundant sites of phosphorylation on 829 proteins. Through the web-based interface, users are allowed to browse identified proteins or search for proteins of interest. Furthermore, we allow users to conduct BLAST searches of the database using both peptide sequences and phosphorylation motifs as queries. The data contained within the database are available for download to be investigated at the user’s discretion. The MPPD will be updated continually with novel phosphoprotein and phosphopeptide identifications, with the intent of constructing an unparalleled compendium of large-scale Medicago phosphorylation data.

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The molecular genetic linkage map of the model legume Medicago truncatula: an essential tool for comparative legume genomics and the isolation of agronomically important genes

Cited by 169 publications

References 74 publications

Physcomitrella Patens Dehydrins (PpDHNA and PpDHNC) Confer Salinity and Drought Tolerance to Transgenic Arabidopsis Plants

Physcomitrella Patens Dehydrins (PpDHNA and PpDHNC) Confer Salinity and Drought Tolerance to Transgenic Arabidopsis Plants

Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future

Medicago PhosphoProtein Database: a repository for Medicago truncatula phosphoprotein data

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