The molecular impact of omega 3 fatty acids on hepatic pro-inflammatory cytokine signaling

Ventro, George; Yang, Yingkui; Chen, Min; Harmon, Carroll M.

doi:10.1016/j.jpedsurg.2017.03.031

Cited by 11 publications

(3 citation statements)

References 12 publications

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“…Recent studies showed that the infusion of fat emulsions based on pure FO containing ω-3 PUFAs (Omegaven ® , Fresenius Kabi, Bad Homburg, Germany) allowed the reduction of the intake of proinflammatory ω-6 and phytosterols while increasing the amounts of a-tocopherol, a powerful antioxidant [ 27 ]. Recently, in a study performed on a culture of human hepatocyte cells, the authors demonstrated the anti-inflammatory and hepatoprotective effect of ω-3 PUFAs by abating inflammatory cytokine signaling such as TGF-β, IL-1, and TNF-α [ 43 ].…”

Section: Risk Factors For Ifald Developmentmentioning

confidence: 99%

Prevention and Treatment of Intestinal Failure-Associated Liver Disease in Children

et al. 2018

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Intestinal failure-associated liver disease (IFALD) is a threatening complication for children on long-term parenteral nutrition because of intestinal failure. When progressive and intractable, it may jeopardize intestinal rehabilitation and lead to combined liver and intestinal transplantation. The institution of dedicated intestinal failure centers has dramatically decreased the incidence of such complication. IFALD may rapidly fade away if very early management aimed at preventing progression to end-stage liver disease is provided. In this review, we address the etiology and risk factors of IFALD in order to introduce pillars of prevention (nutritional management and catheter-related infections control). The latest evidence of therapeutic strategies, such as medical and surgical treatments, is also discussed.

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Section: Risk Factors For Ifald Developmentmentioning

confidence: 99%

Prevention and Treatment of Intestinal Failure-Associated Liver Disease in Children

et al. 2018

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“…We used PCR techniques to detect mRNAs of the PON genes, quantitative Western blot to quantify the proteins, and immunofluorescence and confocal microscopy to analyze their subcellular localization. In order to optimize the methodologies employed, and due to the reduced sample volume of human fresh granulosa cells, we used the stable hepatocarcinoma cell line HepG2, with high PON expression [19,20] along with the human ovary cell line COV434 [21].…”

Section: Resultsmentioning

confidence: 99%

Evidence of Paraoxonases 1, 2, and 3 Expression in Human Ovarian Granulosa Cells

et al. 2021

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Increasing evidence suggests that the antioxidant paraoxonase proteins, PON1, PON2, and PON3, have a role in reproduction and may be synthesized by ovarian cells. The aim of this work was to investigate whether human ovarian granulosa cells (GC) express paraoxonases 1, 2, and 3 (PON1, PON2, and PON3) at both the transcriptional and protein levels. Cells were purified from follicle samples of women undergoing ovarian stimulation at oocyte retrieval. We analyzed mRNA by polymerase chain reaction using specific primers for the different variants and quantified the proteins by Western blot using commercially available human recombinant PON proteins as standards. The protein subcellular distribution was determined by immunofluorescence and confocal microscopy and the cell cycles by flow cytometry. Thymidine was used for cellular synchronization at G1/S. Human hepatoma HepG2 and immortalized granulosa COV434 cell lines were used to optimize methodologies. mRNAs from PON1, the two variants of PON2, and PON3 were detected in GC. The cells actively secreted PON1 and PON3, as evidenced by the protein detection in the incubation medium. PON1 and PON3 were mainly distributed in the cytoplasm and notably in the nucleus, while PON2 colocalized with mitochondria. Subcellular nucleo-cytoplasmic distribution of PON1 was associated with the cell cycle. This is the first evidence describing the presence of mRNAs and proteins of the three members of the PON family in human ovarian GC. This study provides the basis of further research to understand the role of these proteins in GC, which will contribute to a better understanding of the reproduction process.

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“…The results observed in this study support that MPO efficiently affects acute inflammation by inhibiting leukocyte migration, which is possibly mediated by one of its main phytochemicals, phenolic compounds and fatty acids, which are able to reduce inflammatory mediators. In this context, several studies demonstrated that polyunsaturated fatty acids exhibit a protective role in health by decreasing pro-inflammatory mediators (Wiktorowska-Owczarek et al, 2015;Ventro et al, 2017). Murumalla et al (2012) showed that polyunsaturated fatty acids inhibit LPS-induced cytokine secretion, such as IL-6, TNF-α, and MCP-1 in both adipose tissue and mature adipocyte models [36].…”

Section: Figure 3 Lipid Peroxidation Inhibition Induced By Macauba Pulp Oil (Mpo)mentioning

confidence: 99%

Phytochemical profile, anti-inflammatory, antimutagenic and antioxidant properties of Acrocomia aculeata (Jacq.) Lodd. pulp oil

et al. 2020

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The molecular impact of omega 3 fatty acids on hepatic pro-inflammatory cytokine signaling

Cited by 11 publications

References 12 publications

Prevention and Treatment of Intestinal Failure-Associated Liver Disease in Children

Prevention and Treatment of Intestinal Failure-Associated Liver Disease in Children

Evidence of Paraoxonases 1, 2, and 3 Expression in Human Ovarian Granulosa Cells

Phytochemical profile, anti-inflammatory, antimutagenic and antioxidant properties of Acrocomia aculeata (Jacq.) Lodd. pulp oil

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