The molecular manipulation of milk composition

Clark, A. J.; Ali, Simak; Archibald, Alan; Bessos, Hagop; Brown, Paul E.; Harris, Stephen; McClenaghan, M; Prowse, Christopher V.; Simons, Paul; Whitelaw, Bruce; Wilmut, Ian

doi:10.1139/g89-166

Cited by 25 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the first time in livestock, in addition to gene addition, genes could be destroyed (knock-out animals) to generate null alleles. In addition, new transgene designs became available that could result in reduction (knockdown) of the transgene activity, for example those based on RNAi (Clark & Whitelaw, 2003).…”

Section: Brief History Of Gm Milkmentioning

confidence: 99%

Genetically engineering milk

Whitelaw

Joshi

Kumar

et al. 2016

Journal of Dairy Research

View full text Add to dashboard Cite

It has been thirty years since the first genetically engineered animal with altered milk composition was reported. During the intervening years, the world population has increased from 5bn to 7bn people. An increasing demand for protein in the human diet has followed this population expansion, putting huge stress on the food supply chain. Many solutions to the grand challenge of food security for all have been proposed and are currently under investigation and study. Amongst these, genetics still has an important role to play, aiming to continually enable the selection of livestock with enhanced traits. Part of the geneticist's tool box is the technology of genetic engineering. In this Invited Review, we indicate that this technology has come a long way, we focus on the genetic engineering of dairy animals and we argue that the new strategies for precision breeding demand proper evaluation as to how they could contribute to the essential increases in agricultural productivity our society must achieve.

show abstract

Section: Brief History Of Gm Milkmentioning

confidence: 99%

Genetically engineering milk

Whitelaw

Joshi

Kumar

et al. 2016

Journal of Dairy Research

View full text Add to dashboard Cite

show abstract

“…the milk of transgenic animals has been considered as a better source of recombinant therapeutic proteins for the last twenty years. FIX has been expressed in transgenic sheep as a fusion gene comprising the betalactoglobulin and FIX sequences, and small quantities of inactive FIX have been detected in the milk [50]. Higher levels of FIX, secreted in sheep milk, have been achieved using the nuclear transfer technique developed by PPL therapeutics [51].…”

Section: Transgenic Organismsmentioning

confidence: 99%

Coagulation Factor IX for Hemophilia B Therapy

et al. 2012

View full text Add to dashboard Cite

Factor IX is a zymogen enzyme of the blood coagulation cascade. Inherited absence or deficit of the IX functional factor causes bleeding disorder hemophilia B, which requires constant protein replacement therapy. Reviewed herein are the current state in the manufacturing of FIX, improved variants of the recombinant protein for therapy, transgenic organisms for obtaining FIX, and the advances in the gene therapy of hemophilia B.

show abstract

“…FIX has been expressed in transgenic sheep as a fusion gene comprising the beta-lactoglobulin and FIX sequences, and small quantities of inactive FIX have been detected in the milk [50]. Higher levels of FIX, secreted in sheep milk, have been achieved using the nuclear transfer technique developed by PPL Therapeutics [51].…”

Section: Transgenic Organismsmentioning

confidence: 99%