The Molecular Mechanism of Seed Deterioration in Relation to the Accumulation of Protein—Acetaldehyde Adducts

Esashi, Yohji; Kamataki, Akihisa; Zhang, M.

doi:10.1007/978-94-011-5716-2_53

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…Volatiles such as acetaldehyde, ethanol, methanol, and aceton accumulate during seed storage (8). Several studies by Zhang and co-workers have shown a role of ADH in seeds (9,21,22,23). They suggested that the seed deterioration is partly due to the formation of acetaldehyde-protein adducts (APA) during long-term storage in the seed, and suggested that the ADH in seeds is responsible for the detoxification of acetaldehyde to the less harmful ethanol.…”

Section: The Role Of Pme and Adh Enzymes In Soybean Seed Deteriorationmentioning

confidence: 99%

The role of PME and ADH enzymes in seed deterioration and its implication for producing high quality soybean seed

Harnowo,

Prayogo

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Soybean seeds are member of the orthodox seeds group which suseptable to deterioration. The low quality of soybean seeds used by farmers is one of the causes of the low soybean productivity at the farmer level. Pectin methylesterase (PME) and alcohol dehydrogenase (ADH) enzymes have been studied in relation to seed deterioration. Increase in seed moisture content is strongly suspected to be the initial occurrence and increase in PME enzyme activity which then followed by ADH enzyme activity in a series of biochemical reactions leading to the accumulation of acetaldehyde-protein adducts (APA) in soybean seeds. The APA contributes to deterioration of seeds. Thus, the increase in APA in soybean seeds may be used as an indicator of the level of deterioration of soybean seeds. Since high seed moisture content contributes to the initiation of PME activity in seeds, soybean seed production in the rainy season is not recommended because it will produce seeds with low physiological quality due to high PME activity so that the seeds cannot be stored for long-term period.

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Section: The Role Of Pme and Adh Enzymes In Soybean Seed Deteriorationmentioning

confidence: 99%

The role of PME and ADH enzymes in seed deterioration and its implication for producing high quality soybean seed

Harnowo,

Prayogo

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Acetaldehyde dehydrogenase (ADH) in dry seeds can transform acetaldehyde to relatively non-poisonous ethanol (Esashi et al, 1997), which perhaps is a detoxification path in dry seeds. On the other hand, it is also possible that ethanol accumulated within seeds is converted back to acetaldehyde via ADH (Esashi et al, 1997). Therefore, the acetaldehyde content in seeds would also depend upon the interconversion of ethanol and acetaldehyde via ADH.…”

Section: Role Of Seed Moisture Content In Regulating Biochemical Eventsmentioning

confidence: 99%

Fingerprinting of volatile organic compounds for quick assessment of vigour status of seeds

et al. 2020

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Seed is a fertilized mature ovule, which possesses an embryonic plant. When the dry, mature seeds are subjected to imbibition, they release a wide range of organic substances, which include low molecular weight carbonyl compounds (gases and volatiles) and water-soluble organic substances (enzymes and polysaccharides). The volatile organic compounds (VOCs) are molecules of low molecular weight (300 g mol−1) and high vapour pressure (0.01 kPa at 20°C) and include diverse chemical compounds. The nature and emission kinetics of volatiles produced from seeds vary, depending on the moisture content of the seeds. Orthodox seeds stored at ‘low seed moisture content’ undergo seed deterioration, predominantly due to lipid peroxidation, initiated by autoxidation or enzymatic oxidation of unsaturated or polyunsaturated fatty acids. This peroxidation leads to emission of volatile compounds. The quantity of VOCs emitted is positively correlated with the advancement of seed deterioration. With respect to the seed germination process, exposure of seeds to ‘high moisture conditions’ leads to increased respiration, triggers glycolysis and mobilization of storage reserves, resulting in the emission of volatile metabolic products. The quantity of VOCs emitted on commencement of metabolic activity in germinating seeds depends on (1) vigour status and (2) amount of storage reserves. Since it has been established that there is a significant difference between high and low vigour seeds with respect to quantity and profile of VOCs emitted, there is great potential for utilizing the VOC profile to obtain a quick and reproducible test of vigour status of crop seeds. In order to harness the VOC profile for quick assessment of vigour status of seeds, research has to be taken up to develop standard protocols for fingerprinting of VOCs for the purpose of seed vigour assessment and to fix the standard volatile biomarker(s) specific to crop and vigour status of seeds.

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Decrease in beech (Fagus sylvatica) seed viability caused by temperature and humidity conditions as related to membrane damage and lipid composition

Ratajczak

Pukacka

2005

Acta Physiol Plant

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Key words: fatty ac ids, lipid peroxidation, membrane per me abil ity, phospholipids, ster ols, α-tocopherol, vi a bil ity Ab stractThe aim of this study was to de ter mine if the loss of germinability and vi a bil ity of beech (Fagus sylvatica L.) seeds stored at dif fer ent vari ants of tem per a ture (4, 20, and 30 °C) and rel a tive hu mid ity (RH: 45 and 75 %) is as so ci ated with a loss of mem brane in teg rity and changes in lipid com po si tion. Beech seeds stored for 9 weeks grad u ally lost vi a bil ity at a rate de pend ent on tem per a ture and hu mid ity. The harm ful ef fect of tem per a ture in creased with grow ing hu mid ity. The loss of seed vi a bil ity was strongly cor re lated with an in crease in mem brane per me abil ity and with pro duc tion of lipid hydroxyperoxides (LHPO), which was re garded as an in di ca tor of peroxidation of un sat u rated fatty ac ids. The con di tion of mem branes was assessed on the ba sis of their per me abil ity and the state of lipid com po nents: phospholipids and fatty ac ids. Dur ing seed storage we ob served a de cline in con cen tra tion of in di vid ual phospholipids and fatty ac ids, pro por tional to the loss of seeds vi a bil ity. We also de tected a de crease in con cen tra tions of α-tocopherol and ster ols, which play an im por tant role in protec tion of mem branes against the harm ful in flu ence of the envi ron ment. Our re sults show that the germinability of beech seeds de clines rap idly at tem per a ture above 0 °C and grow ing hu mid ity. This is due mainly to the loss of mem brane in teg rity, caused by peroxidation of un sat u rated fatty ac ids.

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The Molecular Mechanism of Seed Deterioration in Relation to the Accumulation of Protein—Acetaldehyde Adducts

Cited by 5 publications

References 7 publications

The role of PME and ADH enzymes in seed deterioration and its implication for producing high quality soybean seed

The role of PME and ADH enzymes in seed deterioration and its implication for producing high quality soybean seed

Fingerprinting of volatile organic compounds for quick assessment of vigour status of seeds

Decrease in beech (Fagus sylvatica) seed viability caused by temperature and humidity conditions as related to membrane damage and lipid composition

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