2017
DOI: 10.1038/srep43692
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The molecular motor Myosin Va interacts with the cilia-centrosomal protein RPGRIP1L

Abstract: Myosin Va (MyoVa) is an actin-based molecular motor abundantly found at the centrosome. However, the role of MyoVa at this organelle has been elusive due to the lack of evidence on interacting partners or functional data. Herein, we combined yeast two-hybrid screen, biochemical studies and cellular assays to demonstrate that MyoVa interacts with RPGRIP1L, a cilia-centrosomal protein that controls ciliary signaling and positioning. MyoVa binds to the C2 domains of RPGRIP1L via residues located near or in the Ra… Show more

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Cited by 35 publications
(37 citation statements)
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“…Interestingly, Myo5a KO resulted in significant reduction in ciliation ( Fig EV3C) suggesting that Myo5a is required for ciliogenesis. This is consistent with a recent study demonstrating that expression of a dominant-negative Myo5a negatively affects ciliation [56]. Immunofluorescent stainings revealed loss of the Myo5a signal around the base of remaining cilia in Myo5a KO cell lines confirming the specificity of the Myo5a staining ( Fig EV3D).…”
Section: Embo Reportssupporting
confidence: 93%
See 1 more Smart Citation
“…Interestingly, Myo5a KO resulted in significant reduction in ciliation ( Fig EV3C) suggesting that Myo5a is required for ciliogenesis. This is consistent with a recent study demonstrating that expression of a dominant-negative Myo5a negatively affects ciliation [56]. Immunofluorescent stainings revealed loss of the Myo5a signal around the base of remaining cilia in Myo5a KO cell lines confirming the specificity of the Myo5a staining ( Fig EV3D).…”
Section: Embo Reportssupporting
confidence: 93%
“…C Venn diagram indicating numerical comparison of cmAPEX proteome with Htr6 interactome. This is consistent with a recent study demonstrating that expression of a dominant-negative Myo5a negatively affects ciliation [56]. Middle and right panel: Numerical comparison of proteins significantly enriched and all proteins identified in our study as compared to Nphp3-APEX [46].…”
Section: Affinity Purification Of Htr6 Determines Actin-binding Protesupporting
confidence: 92%
“…However, actin filaments have been directly observed at the poles of mitotic spindles (Stevenson et al , ; Chodagam et al , ) and at the centrosome of several cell types in interphase (Farina et al , ; Obino et al , ; Au et al , ). Centrosomal actin filaments have been shown to anchor the centrosome to the nucleus (Bornens, ; Burakov & Nadezhdina, ; Obino et al , ), support the transport of vesicles during ciliogenesis (Assis et al , ; Wu et al , ), connect basal bodies to the actin cortex in ciliated cells (Pan et al , ; Antoniades et al , ; Walentek et al , ; Mahuzier et al , ) and power centrosome splitting in prophase (Uzbekov et al , ; Wang et al , ; Au et al , ).…”
Section: Discussionmentioning
confidence: 99%
“…As mentioned earlier, RILPL2 binds MyoVa. Most MyoVa is distributed diffusely throughout the cytoplasm (see below) but a small amount of total cellular MyoVa associates with primary cilia (Kohli et al, 2017) and regulates ciliogenesis (Assis et al, 2017). Wu et al (2018) reported that MyoVa mediates transport of preciliary vesicles from the peri-centrosomal region to the distal appendages of the mother centriole.…”
Section: Myova Regulates Centriolar Rilpl2 Localizationmentioning
confidence: 99%