The molecular systematics of blowflies and screwworm flies (Diptera: Calliphoridae) using<i>28S rRNA, COX1</i>and<i>EF-1α</i>: insights into the evolution of dipteran parasitism

McDonagh, Laura; Stevens, Jamie R.

doi:10.1017/s0031182011001089

Cited by 48 publications

(66 citation statements)

References 99 publications

(183 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, we utilized this opportunity to study the relationship between P. regina and P. terraenovae by incorporating the mtgenome of P. terraenovae from Nelson et al [36] study. Our analyses consistently support the earlier observation, P. regina as a sister taxon to P. terraenovae [59], with high statistical support (bootstrap = 99.5; posterior probability = 1.00). In this study the out-group, C. tepperi converged with suborder Brachycera in both the analyses with low statistical support.…”

Section: Phylogenetic Analysessupporting

confidence: 88%

“…McDonach and Stevens [59] have analyzed the phylogenetic relationships between Calliphoridae species using two nuclear and one mitochondrial protein coding genes and revealed a sister relationship between P. regina and P. terraenovae. But the sister relationship between P. regina and P. terraenovae had never been tested with whole mtgenome sequences due to lack of data.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

See 1 more Smart Citation

A 454 sequencing approach to dipteran mitochondrial genome research

et al. 2015

View full text Add to dashboard Cite

The availability of complete mitochondrial genome (mtgenome) data for Diptera, one of the largest metazoan orders, in public databases is limited. The advent of high throughput sequencing technology provides the potential to generate mtgenomes for many species affordably and quickly. However, these technologies need to be validated for dipterans as the members of this clade play important economic and research roles. Illumina and 454 sequencing platforms are widely used in genomic research involving non-model organisms. The Illumina platform has already been utilized for generating mitochondrial genomes without using conventional long range PCR for insects whereas the power of 454 sequencing for generating mitochondrial genome drafts without PCR has not yet been validated for insects. Thus, this study examines the utility of 454 sequencing approach for dipteran mtgenomic research. We generated complete or nearly complete mitochondrial genomes for Cochliomyia hominivorax, Haematobia irritans, Phormia regina and Sarcophaga crassipalpis using a 454 sequencing approach. Comparisons between newly obtained and existing assemblies for C. hominivorax and H. irritans revealed no major discrepancies and verified the utility of 454 sequencing for dipteran mitochondrial genomes. We also report the complete mitochondrial sequences for two forensically important flies, P. regina and S. crassipalpis, which could be used to provide useful information to legal personnel. Comparative analyses revealed that dipterans follow similar codon usage and nucleotide biases that could be due to mutational and selection pressures. This study illustrates the utility of 454 sequencing to obtain complete mitochondrial genomes for dipterans without the aid of conventional molecular techniques such as PCR and cloning and validates this method of mtgenome sequencing in arthropods.

show abstract

Section: Phylogenetic Analysessupporting

confidence: 88%

Section: Phylogenetic Analysesmentioning

confidence: 99%

A 454 sequencing approach to dipteran mitochondrial genome research

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Comparing all genetic regions and methods utilized, the results presented agree with the assumption that a multigene approach is stronger than single gene analyses (Stevens et al, 2002;Wallman et al, 2005;McDonagh and Stevens, 2011;Zaidi et al, 2011;Nelson et al, 2012) leading to more robust and unambiguous conclusions. The distance method, the phylogenetic topologies, and the likelihood-map results are in agreement with previous observations (Zumpt, 1965;Tantawi and Greenberg, 1993) that the isolated proepimeral seta, in fact, is a weak morphological diagnostic character to differentiate C. albiceps from C. rufifacies, since a few C. albiceps individuals may present it in a polymorphic status.…”

Section: Molecular Aspectssupporting

confidence: 70%

Phenotypic polymorphism of Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae) may lead to species misidentification

et al. 2015

View full text Add to dashboard Cite

Species identification is an essential step in the progress and completion of work in several areas of biological knowledge, but it is not a simple process. Due to the close phylogenetic relationship of certain species, morphological characters are not always sufficiently distinguishable. As a result, it is necessary to combine several methods of analysis that contribute to a distinct categorization of taxa. This study aimed to raise diagnostic characters, both morphological and molecular, for the correct identification of species of the genus Chrysomya (Diptera: Calliphoridae) recorded in the New World, which has continuously generated discussion about its taxonomic position over the last century. A clear example of this situation was the first record of Chrysomya rufifacies in Brazilian territory in 2012. However, the morphological polymorphism and genetic variability of Chrysomya albiceps studied here show that both species (C. rufifacies and C. albiceps) share very similar character states, leading to misidentification and subsequent registration error of species present in our territory. This conclusion is demonstrated by the authors, based on a review of the material deposited in major scientific collections in Brazil and subsequent molecular and phylogenetic analysis of these samples. Additionally, we have proposed a new taxonomic key to separate the species of Chrysomya found on the American continent, taking into account a larger number of characters beyond those available in current literature.

show abstract

“…Several phylogenetic studies have placed species of Hemipyrellia within Lucilia (Wells et al 2007, Park et al 2009, Liu et al 2011, McDonagh and Stevens 2011). Evidence of whether Dyscritomyia is related to Lucilia or nested within it has depended on which gene was analysed (Wells et al 2007, McDonagh and Stevens 2011). The definitions and relationships of these genera therefore need attention.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic radiation of the greenbottle flies (Diptera, Calliphoridae, Luciliinae)

Williams¹,

Lamb²,

Villet³

2016

View full text Add to dashboard Cite

The subfamily Luciliinae is diverse and geographically widespread. Its four currently recognised genera (Dyscritomyia Grimshaw, 1901, Hemipyrellia Townsend, 1918, Hypopygiopsis Townsend 1916 and Lucilia Robineau-Desvoidy, 1830) contain species that range from saprophages to obligate parasites, but their pattern of phylogenetic diversification is unclear. The 28S rRNA, COI and Period genes of 14 species of Lucilia and Hemipyrellia were partially sequenced and analysed together with sequences of 11 further species from public databases. The molecular data confirmed molecular paraphyly in three species-pairs in Lucilia that hamper barcode identifications of those six species. Lucilia sericata and Lucilia cuprina were confirmed as mutual sister species. The placements of Dyscritomyia and Hypopygiopsis were ambiguous, since both made Lucilia paraphyletic in some analyses. Recognising Hemipyrellia as a genus consistently left Lucilia s.l. paraphyletic, and the occasionally-recognised (sub)genus Phaenicia was consistently paraphyletic, so these taxa should be synonymised with Lucilia to maintain monophyly. Analysis of a matrix of 14 morphological characters scored for adults of all genera and for most of the species included in the molecular analysis confirmed several of these findings. The different degrees of parasitism were phylogenetically clustered within this genus but did not form a graded series of evolutionary stages, and there was no particular relationship between feeding habits and biogeography. Because of the ubiquity of hybridization, introgression and incomplete lineage sorting in blow flies, we recommend that using a combination of mitochondrial and nuclear markers should be a procedural standard for medico-criminal forensic identifications of insects.

show abstract

The molecular systematics of blowflies and screwworm flies (Diptera: Calliphoridae) using28S rRNA, COX1andEF-1α: insights into the evolution of dipteran parasitism

Cited by 48 publications

References 99 publications

A 454 sequencing approach to dipteran mitochondrial genome research

A 454 sequencing approach to dipteran mitochondrial genome research

Phenotypic polymorphism of Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae) may lead to species misidentification

Phylogenetic radiation of the greenbottle flies (Diptera, Calliphoridae, Luciliinae)

Contact Info

Product

Resources

About